LOCUS       sequence-215804-       11778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific expression of the GFP living color under the control
            of the Oct4 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11778)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 11778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RIX-F2Oct4F3-GFP
FEATURES             Location/Qualifiers
     source          1..11778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5133..5250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     5270..5286
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5271..5287
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6618..6627
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6624..6626,6627..6629,6630..7343)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6624..6626,6627..6629,6630..7340)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6669..6690)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6930..6949)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7277..7298
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7359..7947
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7412..7432)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7830..7841)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8327..8360)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8380..8560
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8612..8733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8649..8668)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8703..8722
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8752..8887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8919..8938)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8920..8938)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8948..8965)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8948..8964)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8957..8979)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9109..9564
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9196..9215)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9406..9427
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9593..9697
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9698..9766,9767..10558)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9916..9935)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10729..11317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11218..11237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11471..11488
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11605..11626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11641..11646,11647..11664,11665..11671)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11679..11695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11684..11706
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11703..11719
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11703..11719
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11738..11758
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11740..11758
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttgatccca ttactggcct ggtgcttagt tatctttgca agagaatagc cctcagagtg
     1861 cctgtctgca agggatggtg tttgtctgtc tgctcctaca ccatgctctg aacgaaggcc
     1921 ccagggtgaa tcacacagcc tcaaaaagcc cagagcaccc cacagtgtac atagccagga
     1981 cagagttctg agcctgggcc cagcccgcct gagctgttgg ctagggtcag tggctctagg
     2041 tgccctgggg ggcgggggtg tctcttgatg gtagtcttca tcaggtagag atggaacctt
     2101 tctctataaa acaagctgcc cttgaactcc tgataattct cctgcctcag cttcatcgac
     2161 ttcaccaaat tcaccctgag actttgaaaa aaaaattgcg ttttatttta agggtgtgta
     2221 tgtacctaca cacagctaca catagcacat gtgtggaagt cccctccttc taccacatgg
     2281 gtccaagtca tcccccaggg tggctgaggg gccatctctc tggccctctc catgaatctt
     2341 ccgtttcctc cactctgtca tgctcacctc ccaatttcta tacattcatt gtggaacagt
     2401 gccataggtt agttggaaga cctaaataaa ggcagcgact tggaagccgc caagttcaca
     2461 aagcttcctc aatagcagat taaggaaggg ctaggacgag agggacccct ccccaaccat
     2521 cttctctgcc cccagggcag agctatcatg cacctttgtt atgcatctgc cgtctgccca
     2581 gaactctcaa cctccctggg gggaggggca gggtcgggct acaacctccc cacacccagt
     2641 tcctcccagg gccagcctct aaggcctaga cagcactctc caccacagtt caagtatgcc
     2701 tgcagcccag cagtcctgtc tgtattcaat accaaccttg tcttatggat tgtgatttct
     2761 cttttggtga ctcactggcc aggacaagag acatattctg agtcctttaa ctgcctagtg
     2821 actggctttg caaccaggtt agccctaagc gtgcctagag tataatacag tccttaacag
     2881 caactttgtc tgaagtccca agtcttctta gaactagctt gaaacagatg tgccctagcc
     2941 tggtcccgag ctgtgagcct ggtggccctg gagatgggac agcagacctg ttggctcatc
     3001 tgatccagtt tcttgcctcc tgggtcttag aaaatcatca gactaacttt tgggtttgat
     3061 tcaagggttc cctacatagc cttagatggc ttggaactca ctctgtagac caggctggcc
     3121 ttgaactcac agagatccac cagcctttgc ttcctgagtg ctaggattga aaatatgtgc
     3181 cactctgccc cctttaagag tcactttggg ccgggtgcgg tcccaactca tggctttaat
     3241 cccagcactt gggaggcaga ggcaggtgga tttctgagtt ccaggccagc ctggtctaca
     3301 gagtgagttc caggacagcc agggcttcac agagaaatcc tgtctcgaaa aaaaaaaaaa
     3361 atctgtcacc ctcttctggt gtctgaagta cttacgtaca ataataaata aatcttttta
     3421 aaaaaaaaaa agaaagaaag aaaaacaaac aaaaaagagt caccatggta tcttaaatac
     3481 cccaaatttg agctaaaatt cgttaacttg ccacaaacca cctgtatttt agaaccaagt
     3541 caagatctgt ctcggagttc tgtctggaga accaaagaca gcagagaagc ccacacgtcc
     3601 ccagccagag atggtccacc agcatgaaaa ggtatcctgg gaagccagat gcggaatgcc
     3661 acacctataa tgcctgccct tgggcgttgg aagtgataag aacaaaggtc caaggctagg
     3721 ggctgggaaa gaactcagca tttgagtgta cattgctctt tcagaggacc cttttaaaat
     3781 tctgttccta gcacccatat tgggtggttt acagctagga gaacagaaat ctcttggctg
     3841 ctgtggtcat ctgcagttac atggacacac atatatacac atgattttct tttaaagaga
     3901 atttaaggcc agccttaaac acaagagtcc tccaggccag cttgagccag cctcagcagg
     3961 agaccttgtc taaaacaaaa caaacaaaca ataggcgttg acccccacag ctttcttact
     4021 gtgagttcag ccacctcctc acagactggg tgatcagtta aatggactca cggaggacac
     4081 tagactagag cagggaaatc actcgtgtta gcatccaggc tgggtctccc tatgaagcca
     4141 tagggagaaa tgaggagttt cagcgacatg agctcagtca ggcctgcccc agccaggaac
     4201 agcttccttg ctggttagca tccccctccc ccaaccccaa cccccagccg ccctgggacc
     4261 agcaacgtct cttcccaaat ccagatgttc tggaagggag gaggtagttt ctctggctgg
     4321 catagattcc ttagctaact gcatctccct ttccttgtct tggttttctt gcctaatcaa
     4381 aaagacttgc agggggcagg aggggggggg ggggttggag ggggagactt gaggggaggc
     4441 ctgagaggaa cctgaggaca gctcagcaag taaccacact tgctgagcct aacgaccatg
     4501 gatttgatcc ctggggtcca tttcaaagaa gcatttgatc tccagaggtt cgatattgat
     4561 attgcactca gatacacaca cacacacaat acaataaaaa ttaaaagaaa aaacaaagat
     4621 ctagaagctg aaggtaggaa taggtcacca gcagaacaga aggcttgcgg aatgtgcctg
     4681 ctccctttga aataaatgag ttctatttct cccttttttt ttttttcttt ctgagacagg
     4741 gtttctctgt gtagccctgg ctgtcctgga actcactctg tagaccaggc tggccttgag
     4801 ctcagaaatc agcctgccct ctgcttccca agtgctggga ttataggcat tcgccactac
     4861 tgcccgtttc cttttttctt ctgagacaaa tttacatgta gcagaggctg cccctaaact
     4921 ctacgtagcc aagaaggccc ttgaacttct gatcctcttg ccttcccagt tctgatgagc
     4981 agtgtccaaa tgccttttag acttgcagaa atggcttcac cactacaccc aaggctgccc
     5041 ctgataacca gaaccccacg acatcactca ttcaagataa ccagccacct tgatctgcct
     5101 tgtaggtgga gagtctcccc gctaggatca acttttaaaa gaaaaggggg gattgggggg
     5161 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     5221 caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgaggtcgac
     5281 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatcctcaga ctgggcccag
     5341 aaaaccactc tagggaagtt cagggtaggc tctctgcacc ccctcctcct aatcccgtct
     5401 ccttagtgtc tttccgccag cacaggaatg ggggaggggt gggtgacgag gatgaacacc
     5461 ggagtccctg gaggaaggga agcagggtat ctccatctga ggctctgtct ttgaggagag
     5521 gtggagagct ggggaagtct tgtgtgaggg gattggggct caggaggggg ttggggagca
     5581 ggaagttgtc cccaggggag ccatcctggc ccattcaagg gttgagtact tgtttagggt
     5641 tagagctgcc ccctctgggg accaggattg tccagccaag gccattgtcc tgcccccttc
     5701 ccccagtccc tcccaggccc ctttgaacct gaagtcagat atttcttctc tctacccacc
     5761 tcccacccgt tgggtttctc cacccaggaa ctaggctgga agcctgggat gaggaggtgg
     5821 ggggagggag aactgagaat cttgaggaaa gaggccccgg ccttaactgt gaggggatgg
     5881 agcctgggtg caggtcttat gggggttggg gggtggttag tgtctaatct accaacctgg
     5941 acaacacaag atggaatact gtgctctgaa aacgcagagc cagcacttct ctggggtctc
     6001 tggggacata tctggttggg gctcggggtc ccatggtgta gagcctctaa actctggagg
     6061 actggaggtg caatggctgt cttgtcctgg ccttggacat gggctgaaat actgggttca
     6121 cccatatcta ggactctaga cgggtgggta agcaagaact gaggagtggc cccagaaata
     6181 attggcacac gaacattcaa tggatgtttt aggctctcca gaggatggct gagtgggctg
     6241 taaggacagg ccgagagggt gcagtgccaa caggctttgt ggtgcgatgg ggcatccgag
     6301 caactggttt gtgaggtgtc cggtgaccca aggcaggggt gagaggacct tgaaggttga
     6361 aaatgaaggc ctcctggggt cccgtcctaa gggttgtcct gtccagacgt ccccaacctc
     6421 cgtctggaag acacaggcag atagcgctcg cctcagtttc tcccaccccc acagctctgc
     6481 tcctccaccc acccaggggg cggggccaga ggtcaaggct agagggtggg attggggagg
     6541 gagaggtgaa accgtcccta ggtgagccgt ctttccacca ggcccccggc tcggggtgcc
     6601 caccttcccc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     6661 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     6721 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     6781 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     6841 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     6901 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     6961 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     7021 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     7081 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     7141 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     7201 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     7261 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     7321 gcatggacga gctgtacaag taaagcggcc gcgtcgacaa tcaacctctg gattacaaaa
     7381 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7441 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7501 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7561 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7621 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7681 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7741 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     7801 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7861 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7921 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat
     7981 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8041 gctaattcac tcccaacgaa gacaagatgt gatcccccta gagggacagc ccccccccaa
     8101 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     8161 gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc
     8221 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     8281 gcctgcagac acctggggga tacggggaaa aagcttttct agtggaataa cttcgtatag
     8341 catacattat acgaagttat gctgcttttt gcttgtactg ggtctctctg gttagaccag
     8401 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8461 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8521 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagag cggccaaact
     8581 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8641 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8701 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8761 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8821 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8881 ggaggcctag gcttttgcgt cgagacgtac ccaattcgcc ctatagtgag tcgtattacg
     8941 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     9001 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     9061 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta
     9121 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     9181 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     9241 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     9301 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     9361 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     9421 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     9481 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     9541 acaaaatatt aacgtttaca atttcccagg tggcactttt cggggaaatg tgcgcggaac
     9601 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     9661 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     9721 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     9781 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     9841 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     9901 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     9961 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10021 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    10081 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    10141 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    10201 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    10261 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    10321 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    10381 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    10441 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    10501 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    10561 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10621 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10681 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    10741 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    10801 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    10861 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    10921 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    10981 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11041 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    11101 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    11161 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    11221 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    11281 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    11341 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    11401 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    11461 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    11521 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    11581 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    11641 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    11701 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
    11761 acaaaagctg gagctgca
//
