LOCUS       sequence-215808-       10463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the mBmi-1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Salmon P
  TITLE     Generation of human cell lines using lentiviral-mediated genetic 
            engineering.
  JOURNAL   Methods Mol Biol. 2013;945:417-48. doi: 
            10.1007/978-1-62703-125-7_25.
  PUBMED    23097121
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2013"; volume: "945:417-48. doi"; pages: " 
            10.1007/978-1-62703-125-7_25"
COMMENT     Sequence Label: pCLXEBR-pTF-mBmi1
FEATURES             Location/Qualifiers
     source          1..10463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2536..2554
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2577..2595
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2741..2759
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2844..2868
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4066..4090)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     4127..4143
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4128..4144
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4159..4276
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4332..4543
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4562..4957
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4967..5026
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6089..6460
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6486..7074
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6539..6559)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6957..6968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7216..7249)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7269..7449
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7487..7506)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7552..7607
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7606..7625)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7966..7982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7966..7982)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7979..8001)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7990..8006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8014..8020,8021..8038,8039..8044))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8059..8080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8139..8334
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8185..8320
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8340..8474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8377..8396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8431..8450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8542..8559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8713..9301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8793..8812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9472..10263,10264..10332))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10095..10114
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10333..10437)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttcg gtacaactcc
     2521 cactagacat atcctactct atcattgata gagtgaccct tccatagaca tatcctactc
     2581 tatcattgat agagtgaccc ttccactagg ttcacttttc tctatcattg atagggagtg
     2641 gtaaacaatt gatggacaaa atctatcaat gatagagagt gcatgttact agtattgttc
     2701 ttctctatca ttgatagtga ctttgcctaa tcacttagca actctatcat tgatagagtt
     2761 ctagtattag caaggccttt cctctatata aggaagttca tttcatttgg agaagatccc
     2821 cggggatcct ctagagtcga atcacaagtt tgtacaaaaa agcaggctac tagtgaggaa
     2881 ccaattcagt cgactggatc cggtaccgaa ttaattccgg ctcggagtct tttatcaagc
     2941 agaaatgcat cgaacaacca gaatcaagat cactgagcta aatccccact taatgtgtgt
     3001 cctgtgtgga gggtacttca ttgatgccac taccataata gaatgtctac attccttctg
     3061 taaaacatgt attgtacgtt acttggagac cagcaagtat tgtcctattt gtgatgtcca
     3121 ggttcacaaa accagaccac tcctgaacat aaggtcagat aaaactcttc aagatattgt
     3181 atacaaatta gtcccagggc ttttcaaaaa tgagatgaag agaagaagag atttttatgc
     3241 agctcacccg tcagctgatg ctgccaatgg ctccaatgaa gaccgaggag aagttgcaga
     3301 tgaggagaag aggattataa ctgatgatga gataataagc ttgtctattg agttctttga
     3361 tcagagcaga ttggatcgga aagtaaataa agagaagcct aaggaagagg tgaatgataa
     3421 aaggtactta cgatgcccag cagcaatgac tgtgatgcac ttgagaaagt ttctcaggag
     3481 taaaatggac atacccaata ctttccagat tgacgtcatg tatgaagagg aacctttaaa
     3541 ggattactac acgctaatgg acattgccta catttatacc tggagaagaa atggcccact
     3601 acctttgaaa tacagagttc ggccaacttg caaaagaatg aagatgagtc accagaggga
     3661 tggactgacg aatgctggag agctggaaag tgactctggg agtgacaagg ccaacagccc
     3721 agccggaggt gttccctcca cctcttcctg tttgcccagt cccagcactc cagtgcagtc
     3781 tcctcaccca cagttccctc acatttccag taccatgaat ggaaccagca acagccccag
     3841 tgctaaccac caatcttcct ttgccagtag acctcgaaaa tcatcactaa atgggtcatc
     3901 agcaacttca tctggttagg actgttaagg aaaagatttt tcaaccccct gatttagtta
     3961 ccttcattca ttacagcttt atagatgctt aatacatgtg actgtcgtcc agtttgcttc
     4021 cttttgtagt gactttaaat ttggctgctc gagggaatta tctagaccca gctttcttgt
     4081 acaaagtggt gattcgacct gcaggcatgc aagctcgggc cccccctcga ggtcgacggt
     4141 atcgataagc ttgataactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     4201 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     4261 aaaaattcaa aattttccga tcacgagact agcctcgaga agcttgatcg atggctccgg
     4321 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     4381 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     4441 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     4501 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtgtcgt gacgcggatc
     4561 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     4621 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     4681 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     4741 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     4801 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     4861 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     4921 tcgtgaattg ctgccctctg gttatgtgtg ggagggcgga tcaggtcaat gtactaacta
     4981 cgctttgttg aagcttgccg gcgatgttga aagtaacccc ggtcctatgt ccagactgga
     5041 caagagcaaa gtcataaacg gcgctctgga attactcaat ggagtcggta tcgaaggcct
     5101 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
     5161 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
     5221 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
     5281 attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
     5341 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     5401 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     5461 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     5521 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
     5581 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     5641 cggcgggccg gcctcgagcg aattccagta cctgccagat acagacgatc gtcaccggat
     5701 tgaggagaaa cgtaaaagga catatgagac cttcaagagc atcatgaaga agagtccttt
     5761 cagcggaccc accgaccccc ggcctccacc tcgacgcatt gctgtgcctt cccgcagctc
     5821 agcttctgtc cccaagccag caccccagcc ctatcccttt acgtcatccc tgagcaccat
     5881 caactatgat gagtttccca ccatggtgtt tccttctggg cagatcagcc aggcctcggc
     5941 cttggccccg gcccctcccc aagtcctgcc ccaggctcca gcccctgccc ctgctccagc
     6001 catggtatca gctctggccc aggccccagc ccctgtccca gtcctagccc caggccctcc
     6061 tcaggctgtg gccccacctg cccccaagcc cacccaggct ggggaaggaa cgctgtcaga
     6121 ggccctgctg cagctgcagt ttgatgatga agacctgggg gccttgcttg gcaacagcac
     6181 agacccagct gtgttcacag acctggcatc cgtcgacaac tccgagtttc agcagctgct
     6241 gaaccagggc atacctgtgg ccccccacac aactgagccc atgctgatgg agtaccctga
     6301 ggctataact cgcctagtga caggggccca gaggcccccc gacccagctc ctgctccact
     6361 gggggccccg gggctcccca atggcctcct ttcaggagat gaagacttct cctccattgc
     6421 ggacatggac ttctcagccc tgctgagtca gatcagctcc taaagatccg cgtcgtacgg
     6481 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6541 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6601 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6661 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6721 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6781 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6841 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6901 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6961 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7021 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     7081 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7141 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatgggat
     7201 caattcacca tgggaataac ttcgtatagc atacattata cgaagttatg ctgctttttg
     7261 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7321 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7381 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7441 tctctagcag catctagttg aatcgccagt gtgtcgagct agcagatctt tttccctctg
     7501 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     7561 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     7621 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     7681 atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa
     7741 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     7801 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     7861 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     7921 ttatgaagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     7981 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8041 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8101 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
     8161 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8221 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8281 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     8341 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8401 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8461 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8521 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8581 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8641 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8701 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8761 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8821 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8881 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     8941 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9001 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9061 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9121 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9181 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9241 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9301 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9361 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9421 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9481 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9541 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9601 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9661 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9721 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9781 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9841 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9901 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9961 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10021 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10081 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10141 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10201 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10261 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10321 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10381 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10441 atttccccga aaagtgccac ctg
//
