LOCUS       sequence-215813-       12346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mifepristone-inducible lentiviral backbone for Gateway cloning of 
            Gene insert together with constitutive mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 12346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry
FEATURES             Location/Qualifiers
     source          1..12346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             385..642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    689..814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1306..1423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1783..2016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2201..2245
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2602..2626
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2879..3003
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3028..3033,3034..3051,3052..3058)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3112..3771
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3178..3197)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4113..4418
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4297..4316
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4459..4583)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6668..7039
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     promoter        7070..7580
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7499..7517
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7598..8305
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7740..7758)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8008..8027
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8329..8345)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8330..8346)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    8369..8957
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8422..8442)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8840..8851)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(9099..9132)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9152..9332
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9370..9389)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9435..9490
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9489..9508)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9849..9865)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9849..9865)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9862..9884)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9873..9889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9897..9903,9904..9921,9922..9927))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9942..9963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10022..10217
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10068..10203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10223..10357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10260..10279)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10314..10333
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10425..10442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10596..11184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10676..10695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11355..12146,12147..12215))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11978..11997
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12216..12320)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2401 cccaaccccg aggggacccc ttacgcgggt cgaagcggag tactgtcctc cgagtggagt
     2461 actgtcctcc gagcggagta ctgtcctccg agtcgagggt cgaagcggag tactgtcctc
     2521 cgagtggagt actgtcctcc gagcggagta ctgtcctccg agtcgactct agagggtata
     2581 taatggatct cgagatatcg gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     2641 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga
     2701 acggtgcatt ggaacgcgca ttccccgtgt taattaacag gtaagtgtct tcctcctgtt
     2761 tccttcccct gctattctgc tcaaccttcc tatcagaaac tgcagtatct gtatttttgc
     2821 tagcagtaat actaacggtt ctttttttct cttcacaggc caccactaga gtcgaatcac
     2881 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     2941 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     3001 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
     3061 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
     3121 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     3181 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     3241 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     3301 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     3361 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     3421 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     3481 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     3541 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     3601 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     3661 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     3721 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga
     3781 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     3841 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
     3901 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     3961 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     4021 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     4081 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
     4141 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     4201 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
     4261 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     4321 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     4381 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     4441 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     4501 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     4561 tcagctttct tgtacaaagt ggtgattcga cctgcaggtc gaagcggagt actgtcctcc
     4621 gagtttaaaa gcggagtact gtcctccgag gatatcagcg gagtactgtc ctccgagtcg
     4681 cgaagcggag tactgtcctc cgagatcgat gtcgaccccg cccagcgtct tgtcattggc
     4741 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
     4801 ggtgacgcgt gtggcctcga atcgcctgga gacgccatcc acgctgtttt gacctccata
     4861 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc
     4921 cccgtgttaa ttaacaggta agtgtcttcc tcctgtttcc ttcccctgct attctgctca
     4981 accttcctat cagaaactgc agtatctgta tttttgctag cagtaatact aacggttctt
     5041 tttttctctt cacaggccac caagctaccg gtccaccatg gactcccagc agccagatct
     5101 gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa agctcaagtg
     5161 ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt gtcgctactc
     5221 tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg aatcaaggct
     5281 agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg acatgatttt
     5341 gaaaatggat tctttacagg atataaaagc attgttagaa ttcccgggtg tcgaccagaa
     5401 aaagttcaat aaagtcagag ttgtgagagc actggatgct gttgctctcc cacagccagt
     5461 gggcgttcca aatgaaagcc aagccctaag ccagagattc actttttcac caggtcaaga
     5521 catacagttg attccaccac tgatcaacct gttaatgagc attgaaccag atgtgatcta
     5581 tgcaggacat gacaacacaa aacctgacac ctccagttct ttgctgacaa gtcttaatca
     5641 actaggcgag aggcaacttc tttcagtagt caagtggtct aaatcattgc caggttttcg
     5701 aaacttacat attgatgacc agataactct cattcagtat tcttggatga gcttaatggt
     5761 gtttggtcta ggatggagat cctacaaaca cgtcagtggg cagatgctgt attttgcacc
     5821 tgatctaata ctaaatgaac agcggatgaa agaatcatca ttctattcat tatgccttac
     5881 catgtggcag atcccacagg agtttgtcaa gcttcaagtt agccaagaag agttcctctg
     5941 tatgaaagta ttgttacttc ttaatacaat tcctttggaa gggctacgaa gtcaaaccca
     6001 gtttgaggag atgaggtcaa gctacattag agagctcatc aaggcaattg gtttgaggca
     6061 aaaaggagtt gtgtcgagct cacagcgttt ctatcaactt acaaaacttc ttgataactt
     6121 gcatgatctt gtcaaacaac ttcatctgta ctgcttgaat acatttatcc agtcccgggc
     6181 actgagtgtt gaatttccag aaatgatgtc tgaagttatt gctgggtcga cgcccatgga
     6241 attccagtac ctgccagata cagacgatcg tcaccggatt gaggagaaac gtaaaaggac
     6301 atatgagacc ttcaagagca tcatgaagaa gagtcctttc agcggaccca ccgacccccg
     6361 gcctccacct cgacgcattg ctgtgccttc ccgcagctca gcttctgtcc ccaagccagc
     6421 accccagccc tatcccttta cgtcatccct gagcaccatc aactatgatg agtttcccac
     6481 catggtgttt ccttctgggc agatcagcca ggcctcggcc ttggccccgg cccctcccca
     6541 agtcctgccc caggctccag cccctgcccc tgctccagcc atggtatcag ctctggccca
     6601 ggccccagcc cctgtcccag tcctagcccc aggccctcct caggctgtgg ccccacctgc
     6661 ccccaagccc acccaggctg gggaaggaac gctgtcagag gccctgctgc agctgcagtt
     6721 tgatgatgaa gacctggggg ccttgcttgg caacagcaca gacccagctg tgttcacaga
     6781 cctggcatcc gtcgacaact ccgagtttca gcagctgctg aaccagggca tacctgtggc
     6841 cccccacaca actgagccca tgctgatgga gtaccctgag gctataactc gcctagtgac
     6901 aggggcccag aggccccccg acccagctcc tgctccactg ggggccccgg ggctccccaa
     6961 tggcctcctt tcaggagatg aagacttctc ctccattgcg gacatggact tctcagccct
     7021 gctgagtcag atcagctcct aaggatcccc cgggctgcag gaattccacg gggttggggt
     7081 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     7141 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     7201 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     7261 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     7321 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     7381 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     7441 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     7501 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     7561 tcaccgacct ctctccccag ggggatggac cggtgccgtg agcaagggcg aggaggataa
     7621 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     7681 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     7741 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     7801 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     7861 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     7921 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     7981 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     8041 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     8101 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     8161 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     8221 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     8281 cggcatggac gagctgtaca agtaatagga attcgatatc aagcttatcg ataccgtcga
     8341 cctcgagggg gggcccggta cggtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     8401 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     8461 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     8521 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     8581 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8641 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8701 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8761 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8821 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8881 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     8941 ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag
     9001 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     9061 tcccaacgaa gacaagatgg gatcaattca ccatgggaat aacttcgtat agcatacatt
     9121 atacgaagtt atgctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     9181 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9241 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9301 acccttttag tcagtgtgga aaatctctag cagcatctag ttgaatcgcc agtgtgtcga
     9361 gctagcagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     9421 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     9481 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     9541 tttggtttag agtttggcaa catatgccat atgctggctg ccatgaacaa aggtggctat
     9601 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     9661 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     9721 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     9781 cagtcatagc tgtccctctt ctcttatgaa gatccctcga cctgcagccc aagcttggcg
     9841 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9901 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9961 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
    10021 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    10081 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10141 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10201 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
    10261 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10321 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
    10381 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    10441 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    10501 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    10561 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    10621 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10681 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10741 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
    10801 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10861 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10921 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10981 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    11041 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    11101 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    11161 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    11221 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    11281 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    11341 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    11401 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    11461 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11521 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11581 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11641 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11701 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11761 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11821 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11881 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    11941 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    12001 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    12061 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    12121 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    12181 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    12241 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    12301 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
