LOCUS       sequence-215818-        2248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoter J23116 in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::J23116
FEATURES             Location/Qualifiers
     source          1..2248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        115..149
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     terminator      complement(182..268)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(281..299)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        367..471
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(472..540,541..1332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(690..709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1503..2091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1992..2011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtggctcttc gcagttgaca
      121 gctagctcag tcctagggac tatgctagcc catgaagagc gtaagacctc tagggcggcg
      181 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      241 ctttcgactg agcctttcgt tttatttgat gcccgaaagg gcctcgtgat acgcctattt
      301 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      421 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      481 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      541 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      601 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      661 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      721 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      781 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      841 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      901 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      961 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1021 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1081 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1141 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc ccgcggtatc
     1201 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1261 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1321 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1381 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1441 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1501 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1561 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1621 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     1681 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1741 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1801 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1861 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1921 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1981 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2041 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2101 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2161 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2221 cgccgcagcc gaacgaccga gcgccttg
//
