LOCUS       sequence-215828-        2266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminator ECK120033737 in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::ECK120033737
FEATURES             Location/Qualifiers
     source          1..2266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(200..286)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(299..317)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        385..489
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(490..558,559..1350)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(708..727)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1521..2109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2010..2029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtggctcttc ataaggaaac
      121 acagaaaaaa gcccgcacct gacagtgcgg gctttttttt tcgaccaaag gggacgaaga
      181 gcgacctcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
      241 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ccgaaagggc
      301 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      361 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      421 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      481 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      541 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      601 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      661 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      721 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      781 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
      841 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
      901 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
      961 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1021 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1081 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1141 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1201 cgtgggtccc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1261 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1321 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1381 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1441 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1501 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1561 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1621 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1681 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1741 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1801 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1861 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1921 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1981 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2041 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2101 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2161 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2221 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gccttg
//
