LOCUS       sequence-215829-        2299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminator ECK120019600 in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::ECK120019600
FEATURES             Location/Qualifiers
     source          1..2299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(233..319)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(332..350)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        418..522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(523..591,592..1383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(741..760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1554..2142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2043..2062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtggctcttc ataattcagc
      121 caaaaaactt aagaccgccg gtcttgtcca ctaccttgca gtaatgcggt ggacaggatc
      181 ggcggttttc ttttctcttc tcaaggacga agagcgacct ctagggcggc ggatttgtcc
      241 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact
      301 gagcctttcg ttttatttga tgcccgaaag ggcctcgtga tacgcctatt tttataggtt
      361 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      421 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      481 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      541 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      601 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      661 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      721 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
      781 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      841 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      901 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      961 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1021 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     1081 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1141 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1201 tggtttattg ctgataaatc tggagccggt gagcgtgggt cccgcggtat cattgcagca
     1261 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     1321 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1381 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1441 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1501 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1561 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1621 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1681 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1741 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1801 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1861 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1921 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1981 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2041 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2161 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2221 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2281 cgaacgaccg agcgccttg
//
