LOCUS       sequence-215832-        3196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crtE in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::crtE
FEATURES             Location/Qualifiers
     source          1..3196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1130..1216)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1229..1247)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1315..1419
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1420..1488,1489..2280)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1638..1657)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2451..3039
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2940..2959
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtgctcttcg atgcgcccgg
      121 aattactcgc acgcgtgtta agcctgttac cggaaacctc cgcgacgccg gaattggcac
      181 gcttttacgc gctcctgcgc gactatcctc aacgtggtgg caagggcatt cggtcagaat
      241 tactgcttgc ctctgctcgt gcgcacggcc tgtccgagtc agataccggt tgggagtcag
      301 cattatggct ggcggcagcc ttagaactgt ttcagaactg ggtgctggtg cacgatgata
      361 ttgaagatga ttcggaagaa cgccgtggtc gtccggccct gcaccacttg tgtggtatgc
      421 cggtcgctct taacgtgggg gacgcgctgc acgcttacat gtgggctgct gttgggaaag
      481 ccaatgttcc gggagcgttt gaagagtttc tgcagatggt gtaccgcacg gcggaaggcc
      541 agcatctgga tctggcatgg gtggagggtc gtgaatgggg cctgcgtccc gcggattatc
      601 tccagatggt tggcctgaaa accgcacact acacggttat cgtgccgtta cgtctggggg
      661 ccctggcggc aggcatggca ccgcaggacg cgttcacccc agcgggtctg gcgctgggta
      721 ccgcgtttca gattcgtgac gatgtcctca atctggcagg tgatccggtg aagtatggta
      781 aagaaattgg tggcgatctg ttggaaggta aacgtactct gattgtcctg gactggttga
      841 ctacggcgcc ggatgatcgc aaagccatct tcctggacca gatgcgtcgg caccgcgcag
      901 ataaagaccc tgcggtgatc gatgaaattc accgctggct gcttgaaagc ggctctgtgg
      961 aagcggcgca ggactacgcg caggcacaag ccgcggaagg tctggacttg cttgaaaaag
     1021 cattggcaga cgcgccggat gcccaggccg ccgctgcctt actcgcttct gttcgggaac
     1081 tggccacccg cgaaaaataa tgaagagcgt aagacctcta gggcggcgga tttgtcctac
     1141 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     1201 cctttcgttt tatttgatgc ccgaaagggc ctcgtgatac gcctattttt ataggttaat
     1261 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     1321 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1381 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1441 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1501 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1561 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1621 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1681 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1741 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1801 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1861 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1921 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1981 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2041 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2101 tttattgctg ataaatctgg agccggtgag cgtgggtccc gcggtatcat tgcagcactg
     2161 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2221 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2281 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2341 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2401 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2461 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2521 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2581 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2641 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2701 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2761 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2821 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2881 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2941 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3001 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3061 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3121 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3181 acgaccgagc gccttg
//
