LOCUS       sequence-215833-        4069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dxs in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::dxs
FEATURES             Location/Qualifiers
     source          1..4069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(2003..2089)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2102..2120)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2188..2292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2293..2361,2362..3153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2511..2530)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3324..3912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3813..3832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtgctcttcg atgtcttttg
      121 atattgcgaa atatccaacc ctggccctag ttgactcgac tcaggaatta cgcctgctgc
      181 cgaaagagag ccttccaaag ctgtgcgatg agttacgccg ctacctcctg gattctgtta
      241 gtcgtagctc cggccacttc gcatcgggac taggtaccgt cgaactgacg gttgcactgc
      301 attatgtgta taacaccccg ttcgatcaat taatttggga cgtcggtcat caagcatatc
      361 cgcataaaat tctgaccggt cgtcgcgaca agatcggcac aattcgtcaa aaaggtggat
      421 tgcatccttt cccgtggcgc ggcgagtccg aatacgatgt gttgagcgtt ggccattcgt
      481 caacttctat cagcgctggt attggtattg cggtcgccgc ggagaaagaa ggcaaaaatc
      541 ggcgtacggt ttgcgtgatc ggggatggtg caatcaccgc aggcatggca ttcgaagcga
      601 tgaaccatgc gggggacatt cgtccggata tgctagtgat tctgaacgat aacgaaatga
      661 gtatttccga gaacgtgggg gctcttaata accacttagc gcagctgctg agcggtaaac
      721 tttattctag cctgcgcgaa ggcggtaaga aagtgttctc aggcgtccct ccgattaaag
      781 agctccttaa gcgtactgag gaacacatta agggtatggt ggttccaggc accctgttcg
      841 aagaactggg ttttaattac attggtcctg tggacggcca tgacgtgtta ggcttaatta
      901 ccacgttaaa aaacatgcgc gatctgaaag gacctcagtt tctccacatc atgaccaaga
      961 aaggtcgcgg ctatgaaccg gccgagaaag atccgattac cttccacgca gttccgaaat
     1021 tcgacccatc ctccggctgt ctgccgaaaa gcagcggagg cctgccgagc tattccaaaa
     1081 tctttggtga ctggctgtgc gaaactgcgg caaaagataa caaactgatg gccatcaccc
     1141 cggcaatgcg cgaaggttcg ggaatggtgg aattctctcg caagttccca gaccgttatt
     1201 ttgacgttgc tatcgccgag caacatgcag taacctttgc ggctggtctg gcaattggtg
     1261 gttataagcc aattgtggcc atctattcaa cgttcttaca acgtgcatat gaccaggtgc
     1321 tacatgatgt ggccattcag aaactgccgg tcttatttgc catcgatcgt gcggggatcg
     1381 ttggggcaga tggtcagacg catcagggcg ccttcgacct aagttatctg cgctgtatcc
     1441 cagaaatggt gattatgacc cctagcgatg agaatgaatg tcgtcagatg ttatacaccg
     1501 gctatcatta caacgacgga ccatccgctg tgcgctaccc gcgcggaaac gcggtgggcg
     1561 ttgaactgac cccgttagaa aaattaccga tcggaaaagg gattgttaaa cgtcgcggag
     1621 aaaaactcgc tattctgaac ttcggcacac ttatgcccga agcggctaaa gtagcggaaa
     1681 gcctaaacgc aaccttggtg gatatgcgct tcgtaaagcc actggacgaa gccctaatct
     1741 tagagatggc cgcgtcacat gaggcgttag ttacggtaga ggaaaacgct attatgggcg
     1801 gagcgggtag tggtgtgaac gaagttctga tggctcaccg caaaccggtg ccggtactga
     1861 acatcggcct cccggatttt ttcattcccc aaggtactca agaggaaatg cgcgccgagc
     1921 tcggcttaga tgctgcagga atggaagcca aaattaaggc ctggcttgca taatgaagag
     1981 cgtaagacct ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     2041 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcccgaaag
     2101 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2161 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2221 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2281 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2341 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2401 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2461 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2521 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     2581 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2641 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2701 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2761 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2821 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2881 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2941 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3001 gagcgtgggt cccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3061 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3121 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3181 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3241 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3301 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3361 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3421 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3481 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3541 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3601 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3661 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3721 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3781 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3841 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3901 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3961 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4021 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgccttg
//
