LOCUS       sequence-215834-        2983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crtW in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::crtW
FEATURES             Location/Qualifiers
     source          1..2983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(917..1003)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1016..1034)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1102..1206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1207..1275,1276..2067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1425..1444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2238..2826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2727..2746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtgctcttcg atggtccagt
      121 gccagccgtc gtcgcttcat tctgaaaaac tcgttcttct ctcgagcact attcgcgatg
      181 ataaaaacat caacaagggc attttcatcg cctgctttat tttgtttctc tgggccattt
      241 ctctgattct tctgctgtcc attgatactt caattattca taaatctctg ctgggtatcg
      301 caatgctttg gcagacattt ttatataccg gcctcttcat tacagcgcac gatgctatgc
      361 atggcgttgt ttatccgaaa aacccgcgca ttaataattt cattggaaaa ctgacgctga
      421 ttctgtatgg tctgttaccg tataaagatc tgttaaaaaa gcattggctt catcatggtc
      481 atccgggtac cgatctggat ccggattact acaatggcca tccccagaat tttttcttat
      541 ggtacttgca ctttatgaaa agctattggc ggtggaccca aattttcggc cttgtgatga
      601 ttttccacgg actgaaaaat ctggtacata ttccggagaa taatttgatc atcttttgga
      661 tgattccgtc cattttgtcc agtgtgcaac tcttttactt tgggacattc ttaccgcata
      721 aaaaactgga aggcggctat accaatcccc actgcgctcg ctccattccg ctcccgctgt
      781 tctggagctt tgtgacatgt tatcacttcg gctatcataa agaacatcac gaatatcctc
      841 aactcccgtg gtggaaactg cctgaagcac acaaaatctc gctgtaatga agagcgtaag
      901 acctctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
      961 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcccg aaagggcctc
     1021 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     1081 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     1141 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1201 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1261 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1321 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     1381 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1441 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1501 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1561 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1621 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1681 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1741 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1801 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1861 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1921 gggtcccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1981 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2041 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     2101 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2161 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2221 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2281 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2341 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2401 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2461 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2521 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2581 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2641 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2701 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2761 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2821 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2881 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2941 tgagctgata ccgctcgccg cagccgaacg accgagcgcc ttg
//
