LOCUS       sequence-215835-        2734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crtZ in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::crtZ
FEATURES             Location/Qualifiers
     source          1..2734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(668..754)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(767..785)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        853..957
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(958..1026,1027..1818)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1176..1195)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1989..2577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2478..2497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtgctcttcg atgctgtgga
      121 tttggaatgc tctgatcgtg tttgttaccg tcattggcat ggaagttatc gcggctctgg
      181 cccacaaata tattatgcat ggttggggat ggggttggca tttgtcgcac catgagccgc
      241 gcaagggagc gtttgaagtg aacgatctgt atgcagtagt ttttgcggcc cttagcattc
      301 ttctgattta tctgggctcg accggcatgt ggcccttgca atggatcggt gcgggcatga
      361 cggcttacgg cttgctttat tttatggttc acgatggatt agttcaccag cgctggccat
      421 tccgttatat cccacgcaaa ggctatttaa aacgtcttta catggcacat cgtatgcatc
      481 atgcggttcg ggggaaagaa ggctgcgtga gctttgggtt tctctatgcg ccgcctcttt
      541 ctaaactgca agctaccctg cgtgaacggc atggcgcgcg cgcaggcgcg gctcgtgatg
      601 cgcagggtgg cgaagatgaa cccgcatctg gcaaataatg aagagcgtaa gacctctagg
      661 gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
      721 cccagtcttt cgactgagcc tttcgtttta tttgatgccc gaaagggcct cgtgatacgc
      781 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      841 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      901 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      961 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1021 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1081 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1141 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1201 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1261 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1321 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1381 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1441 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1501 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1561 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1621 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcccgc
     1681 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1741 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1801 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1861 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1921 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1981 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2041 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2101 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2161 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2221 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2281 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2341 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2401 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2461 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2521 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2581 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2641 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2701 accgctcgcc gcagccgaac gaccgagcgc cttg
//
