LOCUS       sequence-215837-        3124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  idi in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::idi
FEATURES             Location/Qualifiers
     source          1..3124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1058..1144)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1157..1175)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1243..1347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1348..1416,1417..2208)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1566..1585)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2379..2967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2868..2887
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtgctcttcg atgctgcgct
      121 cgcttctgcg cggtttaact cacatcccgc gcgtcaactc ggcccaacag ccttcatgcg
      181 cacacgcgcg cttacaattt aagctgcgtt cgatgcagat gactctgatg caaccgagta
      241 tctcagctaa cctgtcgcgc gccgaagatc gtactgacca catgcgcggt gcaagtacgt
      301 gggctggtgg tcaatcccaa gatgaactga tgttaaagga cgaatgcatt ttggttgatg
      361 ttgaagataa cattactgga catgcatcaa agttggaatg tcataaattt ctgccgcacc
      421 aacccgcagg tttgttgcac cgcgccttca gtgtatttct gtttgatgac caaggtcgtc
      481 tgttgcttca acaacgggca cgctcgaaaa ttacctttcc gagtgtatgg accaatactt
      541 gttgcagtca tccattgcac ggtcagacgc cagatgaagt ggatcaactg agccaggtcg
      601 cggacgggac cgtcccgggg gctaaggcgg ccgccatccg gaaactggaa catgaactgg
      661 gtatcccggc ccatcaactg ccagcttcag cgtttcgttt cctcacccgt ttgcattact
      721 gtgcggctga cgtgcagccg gcggctacgc agtcagcact ttggggggaa catgaaatgg
      781 attatatcct gttcattcgt gcgaatgtaa cactcgcccc gaatcctgac gaagtggacg
      841 aggtccgtta tgtaacgcag gaagaactgc gccagatgat gcaaccggat aatggccttc
      901 agtggagtcc atggttccgt attattgccg cccggtttct tgaacgttgg tgggccgatc
      961 tggacgcagc actgaacacg gataaacatg aagattgggg taccgtgcat catattaatg
     1021 aggcataatg aagagcgtaa gacctctagg gcggcggatt tgtcctactc aggagagcgt
     1081 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
     1141 tttgatgccc gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     1201 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1261 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1321 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1381 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1441 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1501 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1561 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1621 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1681 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1741 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1801 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1861 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1921 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1981 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2041 aaatctggag ccggtgagcg tgggtcccgc ggtatcattg cagcactggg gccagatggt
     2101 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2161 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2221 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2281 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2521 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2761 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3001 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3061 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3121 cttg
//
