LOCUS       sequence-215842-       10088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector (control) that targets expression to GABAergic 
            inhibitory neurons in rodent cortex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Keaveney MK, Tseng HA, Ta TL, Gritton HJ, Man HY, Han X
  TITLE     A MicroRNA-Based Gene-Targeting Tool for Virally Labeling 
            Interneurons in the Rodent Cortex.
  JOURNAL   Cell Rep. 2018 Jul 10;24(2):294-303. doi: 
            10.1016/j.celrep.2018.06.049.
  PUBMED    29996091
REFERENCE   2  (bases 1 to 10088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.06"; journalName: "Cell Rep"; date: 
            "2018-07-10- 10"; volume: "24"; issue: "2"; pages: "294-303"
COMMENT     Sequence Label: pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)
FEATURES             Location/Qualifiers
     source          1..10088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2049..2166
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2254..2701
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2717..2726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2723..2725,2726..2728,2729..3442)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2723..2725,2726..2728,2729..3439)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2768..2789)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3029..3048)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3376..3397
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3952..4540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4005..4025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4423..4434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4543..4559)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4544..4560)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4650..4883
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4908..4926)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4909..4926)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5216..5235)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5449..5468)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5568..5590
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5628..5646)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5714..5818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5819..5887,5888..6679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6037..6056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6850..7438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7339..7358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7592..7609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7679..7699)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7684..8013
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7864..7999
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9147..9212
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9342..9362
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9787..9921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9824..9843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9878..9897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtgaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     2041 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2101 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2161 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     2221 gaattctcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat
     2281 gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac
     2341 ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg
     2401 aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg
     2461 cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca
     2521 aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca
     2581 ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga
     2641 ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca
     2701 gtcgagagga tccgccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2761 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2821 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatttgca ccaccggcaa
     2881 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     2941 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3001 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3061 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3121 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3181 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3241 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3301 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3361 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3421 catggacgag ctgtacaagt aaactagtgc ggccttaaag agaccggttc actgtgacag
     3481 taaaagagac cggttcactg tgagaatgaa agagaccggt tcactgtgat cggaaaagag
     3541 accggttcac tgtgagcggc cttaaagaga ccggttcact gtgacagtaa aagagaccgg
     3601 ttcactgtga gaatgaaaga gaccggttca ctgtgatcgg aaaagagacc ggttcactgt
     3661 gagcggcctt gaaacccagc agacaatgta gctcagtaga aacccagcag acaatgtagc
     3721 tgaatggaaa cccagcagac aatgtagctt cggagaaacc cagcagacaa tgtagctgcg
     3781 gccttgaaac ccagcagaca atgtagctca gtagaaaccc agcagacaat gtagctgaat
     3841 ggaaacccag cagacaatgt agcttcggag aaacccagca gacaatgtag ctgcggccgc
     3901 gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga taatcaacct
     3961 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4021 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4081 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4141 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4201 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4261 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4321 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4381 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4441 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4501 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     4561 gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     4621 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     4681 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4741 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4801 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     4861 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     4921 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     4981 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     5041 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     5101 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     5161 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     5221 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     5281 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     5341 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     5401 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
     5461 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5521 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5581 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5641 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5701 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5761 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5821 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5881 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5941 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6001 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6061 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6121 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6181 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6241 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6301 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6361 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6421 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6481 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6541 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6601 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6661 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6721 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6781 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6841 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6901 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6961 aactggcttc agcagagcgc agataccaaa tactgtcttt ctagtgtagc cgtagttagg
     7021 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7081 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7141 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7201 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7261 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7321 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7381 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7441 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7501 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7561 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7621 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
     7681 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7741 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7801 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7861 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7921 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7981 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
     8041 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     8101 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
     8161 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     8221 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     8281 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
     8341 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     8401 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
     8461 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
     8521 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     8581 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
     8641 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     8701 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     8761 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     8821 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
     8881 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     8941 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
     9001 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
     9061 gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     9121 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     9181 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     9241 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     9301 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     9361 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     9421 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     9481 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     9541 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     9601 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     9661 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     9721 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     9781 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9841 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9901 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     9961 cccaccccat accctattac cactgccaat tacctagtgg tttcatttac tctaaacctg
    10021 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10081 cttagtag
//
