LOCUS       sequence-215845-        9645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector (control) that targets expression of EGFP to 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Keaveney MK, Tseng HA, Ta TL, Gritton HJ, Man HY, Han X
  TITLE     A MicroRNA-Based Gene-Targeting Tool for Virally Labeling 
            Interneurons in the Rodent Cortex.
  JOURNAL   Cell Rep. 2018 Jul 10;24(2):294-303. doi: 
            10.1016/j.celrep.2018.06.049.
  PUBMED    29996091
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.06"; journalName: "Cell Rep"; date: 
            "2018-07-10- 10"; volume: "24"; issue: "2"; pages: "294-303"
COMMENT     Sequence Label: pHR-hSyn-EGFP
FEATURES             Location/Qualifiers
     source          1..9645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2049..2166
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2254..2701
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2717..2726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2723..2725,2726..2728,2729..3442)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2723..2725,2726..2728,2729..3439)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2768..2789)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3029..3048)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3376..3397
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3508..4096
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3561..3581)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3979..3990)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4099..4115)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4100..4116)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4206..4439
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4464..4482)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4465..4482)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4772..4791)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5005..5024)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5124..5146
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5184..5202)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5270..5374
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5375..5443,5444..6235)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5593..5612)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6406..6994
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6895..6914
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7148..7165
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7235..7255)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7240..7569
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7420..7555
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8703..8768
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8898..8918
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9343..9477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9380..9399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9434..9453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtgaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     2041 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2101 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2161 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     2221 gaattctcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat
     2281 gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac
     2341 ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg
     2401 aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg
     2461 cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca
     2521 aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca
     2581 ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga
     2641 ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca
     2701 gtcgagagga tccgccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2761 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2821 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatttgca ccaccggcaa
     2881 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     2941 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3001 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3061 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3121 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3181 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3241 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3301 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3361 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3421 catggacgag ctgtacaagt aaactagtgc ggccgcgact ctagagtcga cctgcaggca
     3481 tgcaagcttg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     3541 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3601 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3661 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3721 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3781 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3841 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3901 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3961 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4021 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4081 tgggccgcct ccccgcatcg ataccgtcga cctcgaggga attaattcga gctcggtacc
     4141 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     4201 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     4261 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4321 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4381 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4441 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     4501 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     4561 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     4621 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     4681 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     4741 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     4801 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     4861 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     4921 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     4981 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5041 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5101 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5161 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5221 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5281 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5341 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5401 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5461 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5521 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5581 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5641 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5701 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5761 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5821 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5881 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5941 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6001 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6061 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6121 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6181 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6241 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6301 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6361 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6421 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6481 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6541 accaaatact gtctttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6601 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6661 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6721 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6781 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6841 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6901 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6961 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7021 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7081 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7141 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7201 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     7261 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7321 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7381 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7441 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7501 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7561 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     7621 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     7681 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     7741 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     7801 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     7861 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     7921 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     7981 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     8041 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     8101 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     8161 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     8221 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
     8281 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
     8341 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
     8401 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
     8461 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
     8521 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
     8581 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
     8641 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     8701 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     8761 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     8821 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     8881 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     8941 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     9001 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     9061 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     9121 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     9181 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     9241 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     9301 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     9361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     9481 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     9541 gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     9601 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
