LOCUS       sequence-215873-        9475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This lentiviral plasmid expresses MCV tumor T antigens (truncated LT
            and small T) using the viral NCCR (non-coding control region). The 
            sequence was amplifeied from MCV.R17a and truncated LT=428 aa..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Arora R, Rekhi B, Chandrani P, Krishna S, Dutt A
  TITLE     Merkel cell polyomavirus is implicated in a subset of Merkel cell 
            carcinomas, in the Indian subcontinent.
  JOURNAL   Microb Pathog. 2019 Dec;137:103778. doi: 
            10.1016/j.micpath.2019.103778. Epub 2019 Oct 7.
  PUBMED    31600537
REFERENCE   2  (bases 1 to 9475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Pathog."; date: "2019-12"; volume: "137:103778. doi"; pages: " 
            10.1016/j.micpath.2019.103778. Epub 2019 Oct 7"
COMMENT     Sequence Label: pLenti.puro.MCV.ER.RAZ.2
FEATURES             Location/Qualifiers
     source          1..9475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4725..5313
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4778..4798)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5196..5207)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5327..5826
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5362..5385)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5759..5778
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5847..6446
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5847..5866)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6343..6363
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6584..6817
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6889..7010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6926..6945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6980..6999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7050..7185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7205..7224)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7206..7224)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7234..7251)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7234..7250)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7243..7265)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7392..7847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7479..7498)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7689..7710
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7873..7977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7978..8046,8047..8838)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8196..8215)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9009..9475,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgatcctgaa
     2521 aaataaataa ggatacttac tcttttaatg tcctcctccc tttgtaagag aaaaaaaagc
     2581 ctccgggcct cccttgttga aaaaaagtta agagtcttcc gtctccctcc caaacagaaa
     2641 gaaaaaaagt tttgtttatc agtcaaactc cgcctctcca ggaaatgagt caatgccaga
     2701 aaccctgcag caataaaagt tcaatcatgt aaccacaact tggctgccta ggtgactttt
     2761 ttttttcaag ttggcagagg cttggggctc ctagcctccg aggcctctgg aaaaaaagag
     2821 agaggcctct gaggcttaag aggcttaatt agcaaaaaag gcagtatcta agggcagatc
     2881 ccaagggcgg gaaactgcag tataaaaacc actccttagt gaggtagctc atttgctcct
     2941 ctgctctttc tgcaaactcc ttctgcatat agacaagatg gatttagtcc taaataggaa
     3001 agaaagagag gctctctgca agcttttaga gattgctcct aattgttatg gcaacatccc
     3061 tctgatgaaa gctgctttca aaagaagctg cttaaagcat caccctgata aagggggaaa
     3121 tcctgttata atgatggaat tgaacaccct ttggagcaaa ttccagcaaa atatccacaa
     3181 gctcagaagt gacttctcta tgtttgatga ggtcagtaca aaatttcctt gggaagaata
     3241 tggaacttta aaggattata tgcaaagtgg atataatgct agattttgca gaggtcctgg
     3301 gtgcatgctt aagcaactta gagattctaa gtgcgcttgt attagctgta agttgtctcg
     3361 ccagcattgt agtctaaaaa ctttaaagca aaaaaactgt ctgacgtggg gagagtgttt
     3421 ttgctatcag tgctttattc tttggtttgg atttcctcct acttgggaaa gttttgactg
     3481 gtggcaaaaa actttagaag aaactgacta ctgcttactg catctgcacc ttttctagac
     3541 tcctacttcc ttcctctgta agtattagat atggaaaagt ctataaggca aaatatcaaa
     3601 gaaaggttat ttatgacaga ttttctgtac tttcccatct aggttgacga ggcccctata
     3661 tatgggacca ctaaattcaa agaatggtgg agatcaggag gattcagctt cgggaaggca
     3721 tacgaatatg ggcccaatcc acacgggacc aactcaagat ccagaaagcc ttcctccaat
     3781 gcatccaggg gagcccccag tggaagctca ccaccccaca gccagagctc ttcctctggg
     3841 tatgggtcct tctcagcgtc ccaggcttca gactcccagt ccagaggacc cgatatacct
     3901 cccgaacacc atgaggaacc cacctcatcc tctggatcca gtagcagaga ggagaccacc
     3961 aattcaggaa gagaatccag cacacccaat ggaaccagtg tacctagaaa ttcttccaga
     4021 acggatggca cctgggagga tctcttctgc gatgaatcac tttcctcccc tgagcctccc
     4081 tcgtcctctg aggagcctga ggagcccccc tcctcaagaa gctcgccccg gcagcccccg
     4141 tcttcctctg ccgaggaggc ctcgtcatct cagtttacag atgaggaata cagatcctcc
     4201 tccttcacca ccccgaagac ccctcctcca ttctcaagaa agcgaaaatt tggggggtcc
     4261 cgaagctctg caagctctgc tagttcagca agttttacaa gcactccacc aaagccaaaa
     4321 aagaacagag aaactcctgt tcctactgat tttcctattg atctttctga ttatcttagc
     4381 catgctgtat atagtaataa aacagtaagt tgttttgcca tttatactac ttctgataaa
     4441 gctatagagt tatatgataa gattgagaaa tttaaagttg attttaaaag caggcatgcc
     4501 tgtgaattag gatgtatttt attgtttata actttatcaa agcatagagt atctgctatt
     4561 aagaattttt gctctacctt ctgcactata agctttttaa tttgtaaagg agtgaataag
     4621 atgcctgaaa tgtataataa tttatgcaag cccccttaca aattactgca agagaataag
     4681 ccactgctca attaggatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt
     4741 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4801 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4861 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4921 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4981 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5041 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5101 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     5161 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5221 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5281 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctaccgggt aggggaggcg
     5341 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     5401 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     5461 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     5521 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     5581 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     5641 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     5701 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     5761 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     5821 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     5881 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     5941 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     6001 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     6061 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6121 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     6181 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     6241 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     6301 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     6361 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     6421 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     6481 ggagcgcacg accccatgca tctcgagggc ccggtacctt taagaccaat gacttacaag
     6541 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac
     6601 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6661 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6721 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6781 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6841 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6901 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6961 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7021 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7081 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7141 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     7201 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     7261 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     7321 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     7381 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7441 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7501 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7561 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7621 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7681 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7741 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7801 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     7861 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7921 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7981 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8041 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8101 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8161 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8221 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8281 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8341 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8401 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8461 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8521 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8581 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8641 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8701 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8761 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8821 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9121 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggag
//
