LOCUS       sequence-215896-        2758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mTagRFPT-linker sequence for N-terminus tagging of
            human LMNB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-29: LMNB1-mTagRFP-T
FEATURES             Location/Qualifiers
     source          1..2758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1000..1731
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 taaaggctgg tacttggaac ctgcaagccg tgcatttgga acctcggact caagtgccta
       61 ttacgtaatt ccacagcgtc ccggcctcca ggccgtttcc cgagccctcc agcggagcgg
      121 gggataaggt taccacgccc gcggtggccg gggacactct gagtttcgcg tgtggctttt
      181 agggacgttt atatttgaat ttccctgaac cgccgagtgt gggcggtggc gcagatccgt
      241 cccggaaacc tccgggctcc ttcccgcctt tctcaggccc ggcccctcca aggggtcccc
      301 gcggggcggc gggagggccc tgggcccaga gccgcgcggg tgggcagtcc caggcgtcct
      361 tccttacagc cctgagcctg gtccgggaac cgcccagccg ggagggccga gctgacggtt
      421 gcccaagggc cagattttaa atttacaggc ccggcccccg aaccgccgaa gcgcgctgcc
      481 tgctccccat tggcccatgg tagtcacgtg gaggcgccgg ggcgtgccgg ccatgttggg
      541 gagtgcggcg ccgcggcccg cgccacctcc gccccccgcg gcttgcctcc agcccgcccc
      601 tcccggccct cctccccccg cccgccgctc cgtgcagcct gagaggaaac aaagtgctgc
      661 gagcaggaga cggcggcggc gcgaaccctg ctgggcctcc agtcaccctc gtcttgcatt
      721 ttcccgcgtg cgtgtgtgag tgggtgtgtg tgttttctta caaagggtat ttcgcgatcg
      781 atcgattgat tcgtagttcc cccccgcgcg cctttgccct ttgtgctgta atcgagctcc
      841 cgccatccca ggtgcttctc cgttcctcta aacgccagcg tctggacgtg agcgcaggtc
      901 gccggtttgt gccttcggtc cccgcttcgc cccctgccgt cccctcctta tcacggtccc
      961 gctcgcggcc tcgccgcccc gctgtctccg ccgcccgcca tggtgtctaa gggcgaagag
     1021 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
     1081 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     1141 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     1201 tacggcagca gaaccttcat caaccacacc cagggcatcc ccgatttctt taagcagtcc
     1261 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     1321 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     1381 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac
     1441 accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaaccgacat ggccctgaag
     1501 ctcgtgggcg ggggccacct gatctgcaac ttcaagacca catacagatc caagaaaccc
     1561 gctaagaacc tcaagatgcc cggcgtctac tatgtggacc acagactgga aagaatcaag
     1621 gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     1681 ctccctagca aactggggca caaacttaat ggcatggacg agctgtacaa gagcggcctg
     1741 agaagcagag cccaggccag cgcgactgcg acccccgtgc cgccgcggat gggcagccgc
     1801 gctggcggcc ccaccacgcc gctgagcccc acgcgcctgt cgcggctcca ggagaaggag
     1861 gagctgcgcg agctcaatga ccggctggcg gtgtacatcg acaaggtgcg cagcctggag
     1921 acggagaaca gcgcgctgca gctgcaggtg acggagcgcg aggaggtgcg cggccgtgag
     1981 ctcaccggcc tcaaggcgct ctacgagacc gagctggccg acgcgcgacg cgcgctcgac
     2041 gacacggccc gcgagcgcgc caagctgcag atcgagctgg gcaagtgcaa ggcggaacac
     2101 gaccagctgc tcctcaagtg agtgctagct ggcggccgcg ttagcgccaa ggaggggcgg
     2161 gggcgcaacc gcggcgacca gctcaccggg ttctgccgtg gggagggagc agaggccagg
     2221 atgcacgcgt ccttctgaag gaacagggtc tcggtctccg gaaaggagaa agaatctaga
     2281 gttcatagcg gagcaggggt cgcggagggg gctcgagctg tagcgctggg gggccgtgat
     2341 gcccatttct agattttgga tacccgctgg gacgtggtaa gtgcgcgcct gggactgccg
     2401 agaaggagct cccgctttcg cactcgaatc cggggagccg gcgcggagag gcggcccctc
     2461 aggccccagg tgcggggagc tggagcgcga gcgcgcgctc gcgtgcgcgc cccagtttcc
     2521 ggccggcgcg agacaaagcg tctagcggat ttgcagtgcc gggatgggcg gccggggagg
     2581 actggcagcc cgcctctaga atgaatgagc ttcgcgcggg cagagagagg aaggggaggg
     2641 accttcccgc agcatccgcg tctcctgggg gtgggtcccg ctttggcgcg ctcagtcttg
     2701 gccctgtgac gttttgcgaa gattctacgc ctgctttagg cgggagagag aggcggag
//
