LOCUS       sequence-215898-        2723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mEGFP-linker sequence for N-terminus tagging of 
            human ATP2A2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-41: ATP2A2-mEGFP
FEATURES             Location/Qualifiers
     source          1..2723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgcagcccgg gcgaccgagg gcgaggaggc gagccaagga catcagcccg agggcgcctc
       61 gagacgcccc gcgtggaccg cgctcccagc tcctcggcct cgccttccaa ccatccgccc
      121 accggcccca gagcagcgtg cccactgtga gcgccccacc ctgcgtctgc aggtgggtgg
      181 gtcagagaac cgcaggcaca gaagagggta cccagcttcc cctccgccag ccccgcgacc
      241 gcggcgcgcg cggcctcgat ccgggttcct aggggcggcg cgcgggaggg ggcggggcct
      301 gcgcggcagc gtgggcgcca ggcgcgcggg aggagggagc cgggaggagg gggcggggcc
      361 gcgccgcccg cgccgcgctg ggcgctctcg gccaatgagc ggcgtccaca tgccgcggcg
      421 gcggcgaaag gggaggcagc ggccgataaa tgctattaga gcagccgccg cggagccgtc
      481 cccgacgcca cctccttttc cttcgccgca gtttcctccg ccgctgtcgg gcgtgcggcg
      541 ctgagggacc cgggcgagcg cgccgcgcac cgccccgccg gctcgcctcc ctcgccgcgt
      601 tccgccctca gtggtctgcc gggcgccccc tcctccggcc cgggcggggc ctctgatcgc
      661 ctcaagagag cggggagggg gctcgggggc cgcggcctgc cctcccggcg ggcggctgag
      721 ggcgagggag gccctccctt ctggcgaggg gagggagggt gggtcaggag cccccaaccc
      781 gccctgcgga gctcggggcc gcgcgagggg cggttgtctg ggggaggggg cgcggggtga
      841 ttcagcgccc ggcgaggcgg aagcggccgc aagaggagga ggggagagcc cgtccgcgcc
      901 tgggctcccg gggtggcacg agcccgcggc cggagtgcga ggcggaggcg aggaggccgc
      961 ggggacggga ggcgaggccg gccgggcccc cgaagccatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagggcagc gctgagaacg cgcacaccaa
     1741 gacggtggag gaggtgctgg gacacttcgg cgtcaacgag agtacggggc tgagcctgga
     1801 acaggtcaag aagcttaagg agagatgggg ctccaacggt aggtgcaggg cgctccgctg
     1861 caggggcccg gcgcggccgg gagagccagg gaagatggct gaccgggctc cacctcgtgg
     1921 gcttcggctc cgcgcccgcc gacagctgcg ggcggagggt cgggccagcg cgccggcccc
     1981 gcgggagaga aaggggctgc ggtcctcgcc tcgccttccc tggacctctt cgcttctcgg
     2041 gccctcgacc ttttcggcgc gcaaggctcg gaggcttctc tccagcagca gccggccccg
     2101 gtggagggag ggacgtggct tctgcagcta ggttgagccc ggcaagacgt tttctcgtcc
     2161 cctgccgatt tatgaggagt cgaggtcgtt ggaaggcctc tgaccgttct tgtcctaccc
     2221 aaagttacac atctggcaga agtgatgaca tcgctgaaac cactcctagg ttcaggagcc
     2281 cgaagtgatt tcacgcttag ggctagacct caggccattg attacaaaga gtgcatgctt
     2341 ccttgagata tcttcgtggt atgccctctg ctaaaaaaat cggatattct tatagctagg
     2401 tctgtgtttt aaagatattg atgcttagaa ttgtagcagt tctttttaga aaaacgaagt
     2461 gctaaaatgt ctctctcttt ttttttttaa cctccctctt gacacattgc ttgacgaatt
     2521 tctacattct acagagttac cggctgaaga aggtaatctt aacatgctgt ttctgttttt
     2581 tttcctctgt tggtgtgctg atggtaagat gacagttaaa acacatgtgt ttgtttctta
     2641 caggaaaaac cttgctggaa cttgtgattg agcagtttga agacttgcta gttaggattt
     2701 tattactggc agcatgtata tct
//
