LOCUS       sequence-215899-        2741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human SMC1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-57: SMC1A-mEGFP
FEATURES             Location/Qualifiers
     source          1..2741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1028..1030,1031..1744)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1070..1091)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1331..1350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1678..1699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctactggttc cctcccagct acaagccagc ccccttcttc gtcctggatg agattgatgc
       61 tgccttggat aacaccaaca ttggcaaggt gggtgcaaag aaagtggggc ctgggaggga
      121 tgcccccagg tcggggttgg gtttcaggga gcaactcctg agcgtgccct agccgttcct
      181 gctgcctgct ctctgtgcct gggaggctgg gcccagagtc atccatgctg tggtagtcag
      241 tagtctcctc tcaacctctc ttttccccac cttcctctcc ctaccctacc tcttctgcct
      301 tctggttgtg gctggaaaat actggggact gttaaggttt gagccttgct aagtgcctgg
      361 cggcctttcc cacaggtggc aaattacatc aaggagcagt cgacttgcaa cttccaggcc
      421 atcgtcatct ctctcaagga ggagttctac accaaggccg agagcctcat tggagtctat
      481 cctgaggtag ggcgggcctg gctcaggagc cagtcctact ccctgcctga tttcccaggg
      541 caggaaagga aggctgcagt ggaggggaaa ggaggtgggg gagcataggg aatcaggtcc
      601 tgaatggcca tttgggcttg ttggagccct gcctctggag gcttagccag cccagccagg
      661 cagggccatt gcatttgggg acaggtggaa aggcaggcag aggcagccag acagtggaga
      721 aaggaggaga gtacagaagt aggcaaggag cgaaggcaca tgggactgag catatcctct
      781 cacacccaga gagaaccatt gactgggctt tgggcaggta tgtagggagg agggtttgag
      841 gcccctggtc ctggggcact agacccctct taattctctc cttacaattt tatttttctt
      901 tttctccctc ctccttcagc aaggggactg tgtgatcagc aaagtcctga ccttcgacct
      961 caccaagtac ccagatgcca accccaaccc caatgagcag ctgagctcca gcggcccctc
     1021 aggcagcgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1081 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1141 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1201 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     1261 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1321 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1381 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1441 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1501 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1561 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1621 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     1681 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1741 gtagcagtat ttttgtcctc cggccctgtc tggatcccta agctgtccct ctcccaatct
     1801 ctggatattt gactcccaac cttcccccta cctcctggcc ctttttggtg tagtcatggg
     1861 atttaggcac tgctaatcaa gcatgaagag gaacagaggt gatgttaggt ctggagcaaa
     1921 aattcctgaa cgacagggag tattctggcc tctgaaagga ggtgctgagc tgaacagggc
     1981 catctgttca tcacacacac ccccttcctc cccctcatca cccataatcg tgggcccctt
     2041 gggcctcttg cccactgtgt gtgtgggtat gtatgtgtgt atgtatgtat ccgcatgtgt
     2101 gcatgtgagt atgtttgcaa aataataaag gatattggag acctgtttta gaaggagcct
     2161 aggctgaatt tgattccaag agagcttagg atgacagcac ccctgagctg ggcaaaggta
     2221 ctcaggacct cataggagtc ttaggcagtt acctgaaact gccttcattc actcatttgt
     2281 gtattcattc atttatgtat tcatcagaca cataccgaac accctctatt tgtcaggctc
     2341 tgtgcttgga atacagagtt gaatcagaca tgatctctac cctcctagta aggagataca
     2401 gtgggttcat gaatgactat agttagctga atgtcatatg tactttgaat ttgagaagtg
     2461 ggtgatcccc tctaggcttc ctggaggtca catttaagct agaccttgac aaattggtag
     2521 gatttggtca ggcactagga gtggagcatg agctctgggg acagacagtt atgggttctg
     2581 gtcccacttt ttatcactta ctagttgttt gaccttgggc aagtcatttg accttctgtg
     2641 cctcagtttc ctcatctgta aaatggggct aacaatatta cctacctcat aggatttaat
     2701 gatgtcaagc tcctcactgg aggccttatc ccttcgtgga g
//
