LOCUS       sequence-215900-        2729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mEGFP-linker sequence for N-terminus tagging of 
            human NUP153.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-58: NUP153-mEGFP
FEATURES             Location/Qualifiers
     source          1..2729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aacctttata tgtttaaaga aacattaatg aagccgtata tattttctaa ttctaaggat
       61 catggacttt atttttcaac cacagatttt tttttcctcc ttcttaaatc attagctctt
      121 gtctttgatt ttactgtctg cttacttcct gcctacttaa ccaaccgaca gggagaagga
      181 gaatgggaag aggttactgt ttttatgtta agtgtctttt caaagtctaa tttacagcca
      241 agaacagcaa aagtacccct tggctttgtg cctctgcggc gccccggaga tctccttccc
      301 cgtccctctt cctttccgag aagccgcgtt ccgttgttga actacgtttc ccagcgtgcc
      361 gcgcggccga gagcgtggac tcgaccagcc cttcctcttt gcctatcagc tcgtcgcagc
      421 atgatgatgc aaatgcgtcg ggcgccgatg agtgacgaca ttctggctcg cgaccgcggc
      481 agccgcctta gcaggggtaa taggcgcaac ggcggcggag gctgcaggga cgacgacgac
      541 gacggcggcg gggcgggcgc tgtgcgcaca ggggaggggt aggcgggcgt cgccagcggc
      601 ttcaactgcg gcagctacgg ctagggcgac ggaagaactc ccaccagtcg gcggtcgagt
      661 taggcctcag caccgcgggg aactgttcgt gctgtcctct gcgggagatc tccatagaga
      721 ccgtgacaca cacagaggcg ggggtccgca gaggcggccg gaccggagcc tcctcggcct
      781 ctgcggccgc cacccccttc cccgctgcca gcactcaccc tctctccgct cctgctcgca
      841 acctcgcggt tcctggggtg cttgtgccca ctgtgtggac agcgcgggcg gacttttggg
      901 ccggggccgg gcggcggggg aggctctcta aggcctccgc ctctgcctct cccgccccct
      961 tacccgcccc ggagcgggaa gcggcggagg ctccgccatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagtacagc gacctggagg cctcaggggc
     1741 aggaggagtc ggagggggcg gtggcggcaa gatccggacg cggcgttgcc accaggggcc
     1801 aattaagcct taccagcagg ggcgacaaca gcatcaggta tgggaccccg acgccgcggt
     1861 ggcctggcgg gtggggaaac tcggctggag gggacgggcg gttcgaggcc ccggcgcccc
     1921 acgtggagca gacaggaggg cctgaggaaa ggcccgagtg aggctccggg ggagctcagg
     1981 ccgttggggg tggcgacggg aagtgggctg agacaggccc agccttgcgg cgcacacgtg
     2041 tggtttgggc cgcctttccc ccaactctct gggtcagggg ctttcccagg caggctctgg
     2101 gcgggaaaag aaattgtagg cccatgatgc gcgcgtttct ttattttttg gcggggattt
     2161 ggaatttgag ggtattactc atgttctctc gagggagaaa ggaaaagatg tggttgatgg
     2221 cccttatact tgctgttttc tggtgtccct tctttagtgt gtttgaggag ggggggctgc
     2281 cccttggaat cttaactctg gggcagacgg ggagttgtgg ggggcgctgt tacatggaaa
     2341 gtggaataac ttagtctcag cctatgagtt aattctcggt ttcccctgcc tgtggccctc
     2401 ctcgtcttga gagcattggc ttagatttta ttaaccaggt tatagttaac cgtggcactg
     2461 gggcattttg aaagtccctc tccaccctta cgtcttttct ctgctgcgcg actcccctcc
     2521 ccccccccaa caccgccacc cccgccttta ttttggaggc tggaattggc cattctccac
     2581 agttgaccgg gactccagct ctgatctcat acacaaaata ttttaatatt atgaggcgta
     2641 ttctagaagt atagttactt ttgacactcc aggaatgttg gtggtcttat agtttagcca
     2701 ctgtatgaac tcctggatgt ttaaaatta
//
