LOCUS       sequence-215902-        2726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and mEGFP-linker sequence for N-terminus tagging of 
            human CBX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-59: CBX1-mEGFP
FEATURES             Location/Qualifiers
     source          1..2726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1006,1007..1717)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MLVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI
                     KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYK"
     primer_bind     complement(1046..1067)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1307..1326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1654..1675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttgtattttt agtagggaca gggtctcgcc atgttggcca ggatggtctt gaacccctga
       61 cctcaggtga tctgcccgcc tcggcctccc aaagtgctag aattataggt gtgagccact
      121 gcacccagcc tttcaattta atgcaatatt ttagctaccg tgctctttta caacggtttt
      181 tttgtgaaga tggagtcttg ctgtgttgcc taggctggtc acacactact gggcttcagg
      241 aattctccca cttgggcctt ccaaaatgct gggattgcag atgtgagcct gcacgcctgg
      301 ccctagctac catgctttat ggtaaaagga gtattgggag ccttttaaaa ttaccaagaa
      361 tgggtataaa agttttaaat ggatacacaa gaataaagaa gtttgctgtg aaggccaggc
      421 gtggtggctc atgcctgtaa ttccaacact ttgggaggcc gaggcaggtg gatcacctga
      481 ggtcaggagt tcgagaccaa cctggccaac acatagtgaa atcccgtctc tactaaaaaa
      541 aaaaaaaaaa caaaaacaaa aaaaaaaaca acaaaaatta gctgggcttg gtggcacacg
      601 cctgtagtcc cagctgcttg ggaagctgag gcaggagaat cgcttgaacc caggaggcgg
      661 aggttgcagt aagccgagat tgcgccactt tgcactccag cctgggcaac agagcaagac
      721 tccgtctctc aaaaaaaaaa aaaaagtttg ctgtaagatt aattggggct gttttatgag
      781 ctttctggct ggacatatca gaaaactatt ttgtatcgag tgtgagaaga agcctaacac
      841 ttactataag cagcatacaa tattatcacc tgtcatgtcc ctggttttct taccatccag
      901 atattatcta accaataaga gtgctcccta atgccctttt tatttcattt atcattttag
      961 cagcgtcagc ctttacacca gaaagctggc gggcactatg ctggtgagca agggcgagga
     1021 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1081 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1141 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1201 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1261 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1321 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1381 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1441 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1501 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1561 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     1621 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1681 cgccgggatc actctcggca tggacgagct gtacaagaag ggcagcggga aaaaacaaaa
     1741 caagaagaaa gtggaggagg tgctagaaga ggaggaagag gaatatgtgg tggaaaaagt
     1801 tctcgaccgt cgagtggtaa agggcaaagt ggagtacctc ctaaagtgga agggattctc
     1861 agagtaagtt tcactgacac aggtaaatgt agccaccacg tgtttatcag gagtctagag
     1921 atgtatgaga cctccagtgc tatgatggat acagtgtgac tcagtcccca ccttcatggc
     1981 ttattgttta attaggaata tggcctactg cttataatac aaagcagaat gacttctttt
     2041 taaaaattat ttcttttgag acgaggtctc gctctgtcac ccaggctgga gtacagtggt
     2101 gcagtcatag ctcgctgcag cctcaacctc ccaggctcaa ggtatcctcc cacctcagcc
     2161 ttctaagtag ctggtaccac aggcatgcac caccatgtct cgcttagttg cccaggtttg
     2221 tctcttaact cctggcctca agcagtcctc ccacctgggc ctcccaaagt gttgggacta
     2281 caagcatgag ccactgtacc cagcccatag tgattgctga gaaacagttt tgtgtgtggc
     2341 tatagaccaa tttaaattga caaataaaag tcatttgcct ttgaaggaag ccagtttctt
     2401 agctttctat ttatactaaa aagcttcccc attccccaag aatttcagct tttccataga
     2461 catctgaggt cttgtcctct tacgattatc cttagtatga gtatacttat ttgagtgaaa
     2521 gtgtgacttg ctgtgccctc tggtttcagt gaggacaaca catgggagcc agaagagaac
     2581 ctggattgcc ccgacctcat tgctgagttt ctgcagtcac agaaaacagc acatgagaca
     2641 gataaatcag agggaggcaa gcgcaaagct gattctgatt ctgaagataa gggagaggag
     2701 agcaaaccaa agaagaagaa agaaga
//
