LOCUS       sequence-215903-        2882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human FUS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-64: FUS-mEGFP
FEATURES             Location/Qualifiers
     source          1..2882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1001..1021
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1034..1078
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1118..1141
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(1169..1171,1172..1885)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1211..1232)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1472..1491)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1819..1840
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgcgtgctct ttgatatatt ggtactgagg tatgtgcgtg ttttccaaag aagtaaatgt
       61 caaggccaca ctgttggggt cagatttagc caaaagctta cctaggtaag gttgatgtaa
      121 tgggaaaggt aatggattgg gttcagtaat actgattttt gttcctgact ctgagaagca
      181 agccgttttg tctttctgaa gcttcagttt cctcactgta tctctaaagt caccgtagtt
      241 tcttcctagt tctaggtctt gcctattccc catcgctcca gactgattgt cttcctttct
      301 ccttagcacc tgtgagaata tgaacttctc ttggaggaat gaatgcaacc agtgtaaggc
      361 ccctaaacca gatggcccag gagggggacc aggtggctct cacatgggta agaaaggcag
      421 acctggtgct agggagctgg gaccaaagaa tccttaattt ttcagcgggg aggctcgggg
      481 aacatagggg aatgggaata tgatagatct tgtttctttt gtcctagggg gtaactacgg
      541 ggatgatcgt cgtggtggca gaggaggcta tgatcgaggc ggctaccggg gccgcggcgg
      601 ggaccgtgga ggcttccgag ggggccgggg tggtggggac agaggtggct ttggccctgg
      661 caagatggat tccaggtaag actttaaatc agaataaaaa agtagagcag ttgaacagag
      721 gccataggat aacagggttt tgttgagaaa gtggtttcat tttgagggct aggtggaaag
      781 acctgaggtt gtaaccagta gtggagaggg aaggaaaatt aactcagggg gagtgaatct
      841 gtagacccac ttgagataag atactcgctg ggttaggtag gaggggcaga taggatatct
      901 aggcttggag aggctggtaa ctcaaatata atggatactt aatttttttt tttttttttg
      961 caggggtgag cacagacagg atcgcaggga gcgcccgtat gagaatcttt attttcaggg
     1021 cgccgccaaa ttcaaagaaa ccgctgctgc taaattcgaa cgccagcaca tggacagcgg
     1081 aggtggaggt tcgagcggtc cctcgggttc gtcgagcctg gaagttctgt tccaggggcc
     1141 cctgtcgtcg agcggtccct cgggttcggt gagcaagggc gaggagctgt tcaccggggt
     1201 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1261 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1321 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     1381 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1441 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1501 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1561 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1621 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1681 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1741 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     1801 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1861 cggcatggac gagctgtaca agtaattaga ctggctcccc aggttctgga acagcttttt
     1921 gtcctgtacc cagtgttacc ctcgttattt tgtaaccttc caattcctga tcacccaagg
     1981 gtttttttgt gtcggactat gtaattgtaa ctatacctct ggttcccatt aaaagtgacc
     2041 attttagtta aattttgttc ctcttccccc ttttcacttt cctggaagat cgatgtcccg
     2101 atcaggaagg tagagagttt tcctgttcag attaccctgc ccagcaggaa ctggaataca
     2161 gtgttcgggg agaaggccaa atgatatcct tgagagcaga gattaaactt ttctgtcatg
     2221 gggaaagttg gtgtataaat gagaaatgaa gaacatggga tgtcatgagt gttggcctaa
     2281 atttgcccag ctatggggaa tttttccttt accacattta tttgcatact ggtcttagtt
     2341 tatttgcagc agtttatccc tttttaagaa ctctttgatc ttttggccct tttaatggtg
     2401 aggctcaaac aaactacatt taaatggggc agtattcaga tttgaccatg gtggagagcg
     2461 cttagccact ctgggtcttt cacaggaagg agagtaactg agtgctgcag gagtttgtgg
     2521 agtggagtca ggatctagga ggtgagtgac tcccttccta gctgccctgg tgaacagcgc
     2581 ttgggtagat acctgctata aggagactgg tctggctggg ttactttcac atcctgcctg
     2641 tactcagagg gcttcaggtc attgacatta tgagatttta ggcttgatcc ctttttgatt
     2701 ggagggtgga aggccctcct aagggaatga taagtgataa gagggggaag gggttgcagc
     2761 caatgagtta aaaccttaga gcagtgctcc tcagcctctt accatgtggt tgtaaacttg
     2821 cacgtacctg ccaaccagtt atttagcatg ctttttattt tagttacaca gagcgtaaca
     2881 tt
//
