LOCUS       sequence-215906-        5705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Roberts B, Hendershott MC, Arakaki J, Gerbin KA, Malik H, Nelson A, 
            Gehring J, Hookway C, Ludmann SA, Yang R, Haupt A, Grancharova T, 
            Valencia V, Fuqua MA, Tucker A, Rafelski SM, Gunawardane RN
  TITLE     Fluorescent Gene Tagging of Transcriptionally Silent Genes in 
            hiPSCs.
  JOURNAL   Stem Cell Reports. 2019 May 14;12(5):1145-1158. doi: 
            10.1016/j.stemcr.2019.03.001. Epub 2019 Apr 4.
  PUBMED    30956114
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2019.03.001"; journalName: "Stem Cell Reports"; 
            date: "2019-05-14- 14"; volume: "12"; issue: "5"; pages: "1145-1158"
COMMENT     Sequence Label: AICSDP-46: MYL7-mEGFP
FEATURES             Location/Qualifiers
     source          1..5705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    1085..1206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1122..1141)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1176..1195
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        1221..1600
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1602..1879
                     /label=chicken beta-actin promoter
     intron          1881..2898
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2822..2844
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2906..2925
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3126..3144)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3394..3413
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3699..3923
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             join(3992..3994,3995..4708)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4034..4055)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4295..4314)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4642..4663
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcctgcggg ggacctggga tgctggagac atggatgctc acctggctgc ctcggccttc
       61 cagggacaga ccccgaggaa gccatcctga gtgccttccg catgtttgac cccagcggca
      121 aaggggtggt gaacaaggat gagtaagtat gggcccagcc agatgaggag caccgtggtg
      181 gaagcagaga gcggggtgag gcccctagtg aggggggctg cctgtgcttc ggggccttac
      241 actgctcttt ggggtgcagc caacccttcc ctgcgccatg ggagcctccg tacccacctt
      301 ccctgtgcag tcactccccc gcagtctcct gctcagaccc tcctcacccc ccaggttcaa
      361 gcagcttctc ctgacccagg cagacaagtt ctctccagct gaggtgaggc tgcccagccc
      421 cttcaatact catccccagc accttctctg ggccttcacc catgacccag agcccagtac
      481 cagtgaggca gttgctggaa gggtgagccg agggcccttc tggaggaggt gccatctctg
      541 ttgagaccta gagggtaaag atgtggagtc agaaaagagg gcagggtgcg ccaggcaggg
      601 agactgtgca cagacctggg gggaagtgga tagggagagg tttcgtacac tcggggtggg
      661 cctgtgcctg tggctggagg ggcgtccttt gccccttggc ccacatttgc actgactcct
      721 cactctgccc agagtcagcc aagagaaaaa cattaaccca gagtctgggg tctagggttg
      781 aaaagctaag gcaaaaagca cagatgcagg gggcagacag aaaggccaca ggactcaggt
      841 gaggtctctg ccgggctggg ccaggagcca ggggactgcc actcaccagt gtcccctgca
      901 ggtggagcag atgttcgccc tgacacccat ggatttggcg gggaacatcg actacaagtc
      961 actgtgctac attattacac atggagacga gaaagaggag gggtccgggt cccccggaga
     1021 ggttcccgac ataccaataa cttcgtatag tgtaggatat acgaagttat attagtattc
     1081 cgataacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1141 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     1201 atcttatcat gtctggtctc gacattgatt attgactagt tattaatagt aatcaattac
     1261 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     1321 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     1381 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     1441 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     1501 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     1561 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     1621 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     1681 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc
     1741 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
     1801 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     1861 gcgaagcgcg cggcgggcgg ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc
     1921 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
     1981 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt
     2041 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
     2101 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg
     2161 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg
     2221 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac
     2281 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
     2341 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
     2401 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
     2461 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc
     2521 ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
     2581 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa
     2641 tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc
     2701 aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct
     2761 ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg
     2821 gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc
     2881 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
     2941 ggcaaagaat tccgccaccg gggatccacc ggagcttacc atggtctcca aaggagaaga
     3001 agataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     3061 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3121 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     3181 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     3241 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3301 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     3361 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     3421 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     3481 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     3541 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     3601 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     3661 caccggcgga atggatgaac tctataaatg aggtactcct gtgccttcta gttgccagcc
     3721 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3781 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3841 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3901 tggggatgcg gtgggctcta tggaaataac ttcgtatagt gtaggatata cgaagttata
     3961 taggtatgtc gggaacctct ccgggtccgg ggtgagcaag ggcgaggagc tgttcaccgg
     4021 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     4081 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     4141 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     4201 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4261 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4321 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4381 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4441 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     4501 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     4561 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
     4621 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4681 tctcggcatg gacgagctgt acaagtgagg ggcagggcca ggcccacggg ggggcacctc
     4741 aataaactct gttgcaaaat tggaattgct gtggtgtctt gtctgtgaca gatgcgttgg
     4801 ggaccagcca agggggatcc cagggtctca gtgcgcacat caccatgatc atggccacca
     4861 tctacctcct gggagctggc ccctcgccag ctcaccttga ttcactccca tgatgccaag
     4921 tgaagtgtga actatgatca tgcctagttt acagatgagg acactgaggc ccagaaagtg
     4981 tgagcatctt accaaggcca gccctctaga agaggagatg gtgggattta caccacctcc
     5041 accaagccca ggaatgagcc acaaagtggg cactgcccag ctacttgggg ctgtgcagag
     5101 aagaggctgc ttgctgggca ctcagcaaac tctgcccaac agcccagcgg gtgggcagca
     5161 gccctgggac ccccacaccc aaccacacag cctcccctgg cccactgctc gcaccccatc
     5221 tcaatacact ggcttgggtg cctccctgca tgggcccttt gtgaaaggca gagaggtacc
     5281 catttgaaac acaaccagct tctcattgca aatacaggca aggcactaag acatgaggaa
     5341 catggacacc aaagcagggg ccaggtaaca tgcaaatttc tagaggaaat gcccagaacc
     5401 tggcatcatg cctcctgagc ccctcatgcg ccgtgagggg taagagggtc agacagctgg
     5461 agtgtaggga gacgacttct caggagagaa tagttagtgc tcccgtcacc cttcatctga
     5521 gaacccaaga gctagaggag aaagtgatcc tcatgagtac cagaggagca gcaggggaca
     5581 tccaaagcac cagagagaga aacagagaca gagagacagg cagtgacagc tcaaacctca
     5641 gccagatcca gagcatacaa agtctcctgc ctacaggaca gcccagtaag agctctcagc
     5701 ttgcc
//
