LOCUS       sequence-215917-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for T7 transcription of wt actin pre-mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(WT)
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(582..601)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(583..601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(652..674)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(883..900)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gnngncnnnt ctagagganc cccttcntca ccaacgtagg agtctttttg acccataccg
       61 accatgatac cttggtgtct tggtctaccg acgatagatg ggaagacagc acgaggagcg
      121 tcgtcaccgg caaaaccggc tttacacata ccagaaccgt tatcaataac caaagcagca
      181 acctctaaac atataatata gcaacaaaaa gaatgaagca atcgatgtta gtacatgaga
      241 cacagtagca aataaaagaa gaatatcact atcacttatc acgaaaatat ttcagttttt
      301 ttttttttcc aaatttcaag cccctattta ttccaataat atcgtggtta ttacagatca
      361 gtcaatatag gaggttatgg gagagtgaaa aatagtaaaa aaaggtaaaa gagaaatctc
      421 tcgagcaatt gggaccgtgc aattcttctt acagttaaat gggatggtgc aagcgctaga
      481 acataccaga atccattgtt aattcagtaa attttcgatc ttgggaagaa aaaagcagta
      541 agcgtgaaaa atctaaaagg ggcgagctcg aattccggtc tccctatagt gagtcgtatt
      601 aatttcgata agccagctgg gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
      661 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
      721 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
      781 tagcgcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
      841 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
      901 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggngg taatacggtt
      961 atccacagaa tcnggggata acgc
//
