LOCUS       sequence-215918-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for T7 transcription of wt actin pre-mRNA (del6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(WTdel6)
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(601..617)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(649..667)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(688..704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(688..704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(701..723)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    712..728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(736..742,743..760,761..766))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    782..803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(920..937)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 annnnnnagg gcgattggag ctcgcccctt ttagattttt cacgcttact gcttttttct
       61 tcccaagatc gaaaatttac tgaattaaca atggattctg gtatgttcta gcgcttgcac
      121 catcccattt aactgtaaga agaattgcac ggtcccaatt gctcgagaga tttctctttt
      181 accttttttt actatttttc actctcccat aacctcctat attgactgat ctgtaataac
      241 cacgatatta ttggaataaa taggggcttg aaatttggaa aaaaaaaaaa actgaaatat
      301 tttcgtgata agtgatagtg atattcttct tttatttgct actgtgtctc atgtactaac
      361 atcgattgct tcattctttt tgttgctata ttatatgttt agaggttgct gctttggtta
      421 ttgataacgg ttctggtatg tgtaaagccg gttttgccgg tgacgacgct cctcgtgctg
      481 tcttcccatc tatcgtcggt agaccaagac accaaggtat catggtcggt atgggtcaaa
      541 aagactccta cgttggtgat gaaggggatc ctctagagtc gacctgcagc ccaagcttat
      601 cgataccgtc gacctcgtcg agggggggcc cggtaccagc ttttgttccc tttagtgagg
      661 gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      721 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct gggggtgcct
      781 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      841 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc gggganaggc ggtttgcgta
      901 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      961 gagcggtatc agctcactca a
//
