LOCUS       sequence-215929-        9354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression and purification of KPNB1_HEAT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Teyra J, Kelil A, Jain S, Helmy M, Jajodia R, Hooda Y, Gu J, D'Cruz 
            AA, Nicholson SE, Min J, Sudol M, Kim PM, Bader GD, Sidhu SS
  TITLE     Large-scale survey and database of high affinity ligands for peptide
            recognition modules.
  JOURNAL   Mol Syst Biol. 2020 Dec;16(12):e9310. doi: 10.15252/msb.20199310.
  PUBMED    33438817
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2020-12"; volume: "16(12):e9310. doi"; pages: " 
            10.15252/msb.20199310"
COMMENT     Sequence Label: pHH0103_KPNB1_HEAT
FEATURES             Location/Qualifiers
     source          1..9354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..22,23..29)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     42..64
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             138..155
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             171..824
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     782..804
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             831..854
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             867..884
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     terminator      3549..3596
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      complement(5189..5644)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5326..5347)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5538..5557
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(5686..5708)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5794..5934
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5849..5868
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5949..5966)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6120..6707)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6878..7669,7670..7738))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7501..7520
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7739..7843)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7911..7929
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7952..8029
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8030..9112
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8049..8068)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca
       61 caggaaacag ccagtccgtt taggtgtttt cacgagcact tcaccaacaa ggaccataga
      121 ttatgaaaat cgaagaacac catcaccatc accattccag cggtaagctt atgtccccta
      181 tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt ttggaatatc
      241 ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa tggcgaaaca
      301 aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat ggtgatgtta
      361 aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac atgttgggtg
      421 gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg gatattagat
      481 acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt gattttctta
      541 gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa
      601 atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat
      661 acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg
      721 aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc
      781 agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat ctggaagttc
      841 tgttccaggg gcccctgtcc agcggtctgg ttccgcgtgg ttccggtacc gcggcccagc
      901 cggccatgga actgattacc atcctggaaa aaacggttag cccggatcgt ctggaactgg
      961 aagcggccca gaaatttctg gaacgcgcag ctgtggaaaa cctgccgacc ttcctggtcg
     1021 aactgtcccg tgtgctggcg aacccgggca attcacaggt ggcccgcgtt gcggcaggtc
     1081 tgcaaattaa aaattcgctg accagcaaag acccggatat caaagcacag tatcagcaac
     1141 gttggctggc tattgatgcg aacgcccgtc gcgaagttaa aaattatgtc ctgcagaccc
     1201 tgggcaccga aacgtaccgc ccgagctctg catctcagtg cgttgctggt attgcatgtg
     1261 ctgaaatccc ggtcaaccag tggccggaac tgattccgca actggtcgcg aatgtgacca
     1321 acccgaattc gacggaacat atgaaagaaa gcaccctgga agccattggc tatatctgtc
     1381 aggacatcga tccggaacag ctgcaagata aatctaatga aattctgacg gcgattatcc
     1441 agggtatgcg taaagaagaa ccgagtaaca atgtgaaact ggcagctacc aacgcgctgc
     1501 tgaattcgct ggaatttacg aaagccaact tcgataaaga aagcgaacgc cactttatca
     1561 tgcaggtggt ttgcgaagcc acccaatgtc cggatacgcg tgtccgtgtg gcagcactgc
     1621 agaatctggt gaaaatcatg agcctgtact accaatacat ggaaacctac atgggcccgg
     1681 cactgtttgc tattacgatc gaagcgatga aatccgacat tgatgaagtc gccctgcagg
     1741 gtatcgaatt ctggtcaaac gtgtgcgacg aagaaatgga tctggcgatc gaagcctctg
     1801 aagcagctga acagggccgt ccgccggaac ataccagtaa attctatgca aaaggtgctc
     1861 tgcagtacct ggttccgatt ctgacccaga cgctgaccaa acaagatgaa aacgatgacg
     1921 atgacgattg gaatccgtgc aaagcagccg gtgtgtgtct gatgctgctg gcaacctgct
     1981 gtgaagacga tatcgttccg catgtcctgc cgtttattaa agaacacatc aaaaatccgg
     2041 actggcgtta tcgcgatgca gctgttatgg cgttcggctg tattctggaa ggtccggaac
     2101 cgtcccagct gaaaccgctg gtgattcaag ccatgccgac cctgatcgaa ctgatgaaag
     2161 acccgtcagt cgtggttcgt gataccgcag catggacggt tggtcgtatt tgcgaactgc
     2221 tgccggaagc agctatcaac gatgtctacc tggcgccgct gctgcagtgt ctgatcgaag
     2281 gtctgtctgc ggaaccgcgc gtggccagta atgtttgctg ggcatttagt tccctggctg
     2341 aagcggccta tgaagcagct gatgttgcag acgatcagga agaaccggct acctactgtc
     2401 tgtcatcgag cttcgaactg attgtgcaga aactgctgga aaccacggac cgtccggatg
     2461 gccatcaaaa caatctgcgc tctagtgcgt atgaatctct gatggaaatc gttaaaaaca
     2521 gtgcgaaaga ttgttacccg gccgttcaga aaaccacgct ggtcattatg gaacgtctgc
     2581 agcaagtgct gcagatggaa tcgcatattc aatccacctc agaccgcatc cagtttaacg
     2641 atctgcaaag cctgctgtgc gcgacgctgc agaatgtcct gcgtaaagtg cagcaccaag
     2701 acgcactgca aatttccgat gtcgtgatgg cttcactgct gcgcatgttt cagtctaccg
     2761 ccggtagtgg cggtgtgcaa gaagatgcgc tgatggccgt ttccacgctg gttgaagtcc
     2821 tgggcggtga attcctgaaa tatatggaag cctttaaacc gttcctgggc attggtctga
     2881 aaaattatgc agaataccag gtgtgcctgg cagcagtggg cctggttggt gacctgtgtc
     2941 gtgcgctgca gagcaacatt atcccgtttt gcgatgaagt tatgcaactg ctgctggaaa
     3001 atctgggcaa cgaaaatgtt caccgctccg tcaaaccgca gattctgtca gtgtttggtg
     3061 atattgcgct ggccatcggc ggtgaattca aaaaatatct ggaagttgtc ctgaacaccc
     3121 tgcagcaagc atcgcaggct caagtcgaca aaagcgacta tgatatggtg gattacctga
     3181 atgaactgcg tgaatcgtgc ctggaagcgt acaccggtat cgtgcagggc ctgaaaggtg
     3241 accaagaaaa cgttcatccg gatgtcatgc tggtgcagcc gcgcgtggaa tttattctga
     3301 gtttcattga ccatatcgca ggcgacgaag atcacaccga tggtgtggtt gcttgcgcag
     3361 ctggcctgat cggtgacctg tgtacggcat ttggcaaaga tgtgctgaaa ctggttgaag
     3421 cgcgtccgat gattcacgaa ctgctgaccg aaggtcgtcg cagcaaaacg aacaaagcaa
     3481 aaacgctggc gacctgggcc acgaaagaac tgcgcaaact gaaaaatcag gccgcggccg
     3541 cataataaac cgatacaatt aaaggctcct tttggagcct tttttttgga gattttcaac
     3601 gtgaaaaaat tattattcgc aattccttta gttgttcctt tctattctca ctccgctgaa
     3661 actgttgaaa gttgtttagc aaaaccccat acagaaaatt catttactaa cgtctggaaa
     3721 gacgacaaaa ctttagatcg ttacgctaac tatgagggct gtctgtggaa tgctacaggc
     3781 gttgtagttt gtactggtga cgaaactcag tgtctagcta gagtggcggt ggctctggtt
     3841 ccggtgattt tgattatgaa aagatggcaa acgctaataa gggggctatg accgaaaatg
     3901 ccgatgaaaa cgcgctacag tctgacgcta aaggcaaact tgattctgtc gctactgatt
     3961 acggtgctgc tatcgatggt ttcattggtg acgtttccgg ccttgctaat ggtaatggtg
     4021 ctactggtga ttttgctggc tctaattccc aaatggctca agtcggtgac ggtgataatt
     4081 cacctttaat gaataatttc cgtcaatatt taccttccct ccctcaatcg gttgaatgtc
     4141 gcccttttgt ctttggcgct ggtaaaccat atgaattttc tattgattgt gacaaaataa
     4201 acttattccg tggtgtcttt gcgtttcttt tatatgttgc cacctttatg tatgtatttt
     4261 ctacgtttgc taacatactg cgtaataagg agtcttaatc atgccagttc ttttggctag
     4321 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac
     4381 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga
     4441 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc
     4501 atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg
     4561 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
     4621 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
     4681 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
     4741 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
     4801 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga
     4861 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc
     4921 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc
     4981 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag
     5041 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag
     5101 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac
     5161 gctgatgagc tttaccgcag gatccgcgaa attgtaagcg ttaatatttt gttaaaattc
     5221 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     5281 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     5341 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     5401 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     5461 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     5521 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     5581 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     5641 gcgtcgcgga tcctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     5701 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     5761 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     5821 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     5881 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     5941 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6001 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6061 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6121 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6181 cgaaacccga caggactata aagataccag ggtttccccc tggaagctcc ctcgtgcgct
     6241 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6301 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6361 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6421 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6481 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6541 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6601 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6661 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6721 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6781 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6841 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6901 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6961 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7021 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7081 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7141 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     7201 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7261 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7321 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7381 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7441 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     7501 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7561 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7621 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7681 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7741 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7801 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7861 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     7921 tcacgaggcc ctttcgtctt caagaattcc cgacaccatc gaatggtgca aaacctttcg
     7981 cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt
     8041 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt
     8101 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga
     8161 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat
     8221 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa
     8281 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt
     8341 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat
     8401 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc
     8461 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga
     8521 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct
     8581 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata
     8641 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc
     8701 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt
     8761 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt
     8821 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc
     8881 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     8941 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     9001 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9061 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9121 caattaatgt aagttagctc actcattagg cacaattctc atgtttgaca gcttatcatc
     9181 gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct
     9241 gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata
     9301 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctg
//
