LOCUS       sequence-215962-        5438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid containing Ple343 (TUBB3 MiniPromoter) 
            insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2176
FEATURES             Location/Qualifiers
     source          1..5438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3217..3233)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3217..3233)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3230..3252)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3241..3257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3265..3271,3272..3289,3290..3295))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3310..3331
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3448..3465)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3619..4207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3699..3718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4378..5169,5170..5238))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5001..5020
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5239..5343)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5411..5429
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga ttacctcgcg aatgcatcta gatggccggc ccctgcgctg ggagccgagc
      481 ccccggcagc tcgcggggat gcagtaagcc gggcgcagcc cgccgggagg atgagaaaac
      541 ccgaagcccg gaaagccgag cggccgcctg cagtcacccc ggggggggca ggggcgggcc
      601 gggctatgca gaaacaccgg ggcccgcggg acacaggacg ctgccccact gccgcagagc
      661 tgaatctgga cccccgggct ttgctttcct ggggcctgtg ctcctccctc cccggggcgg
      721 ggttcgcctg cagcggcgga ggggggagcc tttgtcctcc gcgcgggctg cggcaccgcc
      781 tcctcgcggc tgcggggcgg ggctgggggc ggggcctggc cgtctcagcc aatgggaggc
      841 ggagtccctg gctcgccccg ccctctcgcc tgaaagacct gcggagccgc gggccgggcg
      901 ggaaccgcat tcgggtcctg gaggggcttg gaggccccag tctcgcagcc ccttcatcgg
      961 ggacccccgc tcccacctgc cttggccact gccctcaaag cccggccccg ctttccccag
     1021 gaccccaagc gctgcgcgag gtcagcacca aggacagcgc ccgggccgag cgcctggcct
     1081 cgaacgccgg gttctgcttt gtgtttccag gtttttgttt ttggttatga gaggaggcct
     1141 ggctctgtcg cccgggctgg cgtccagagg cgcgatctcg gctcactgca gcctcggcct
     1201 cccgagtagc tgggaccgca ggcgctcacc accacgcctg gccacaattt tccttttttc
     1261 ttattttctt ttagatggag tttggctctt gttgcccagg ctgggggacg tcgtgtcccc
     1321 tggtcctggg aggctgtccc tggcccaagg tcacacagca ggtgcgagag agctgggctg
     1381 gcccttggtt ctcatcccct gcaggagcca acctggggct cgggcggggc gggttggggg
     1441 cgctgcttgc caaaggatct gccctccgaa cagtgttggg gaacagggga tcgggggggg
     1501 aggctggctt tgtcaggacc aaggccaggg acccagctca gggctggtgc agcgctgatt
     1561 ctgggaagga gggggtgcag gagacggtcc tggaggggaa ggggccagag aaaggcagcc
     1621 tccggaggct gttgccatgg aaaccaggca ggaacaaaaa gctaattaca acagggccag
     1681 ccacgtggct gacatgtaaa ttgcaacttt ggctccgggt ggaggcgcct ctgagggtgg
     1741 gaggcccagt agtggggaga ctctaatcac ccaaggagac agctgtgcct gccaaggaga
     1801 gcctggggga ggggagacct gggggacagt ggcctcaaat caaaccagga ctcccggagc
     1861 agcctctgac agctgccttg gcctgatggg aggggctgag ggctcattaa tgtggtcact
     1921 ggggccaagc ctcccctgag catctgctcc tgggagggtc aggggtcgct ggggggaggg
     1981 tcgcagctgc tcattttgtg aagtgaatgc tgatttgggg gccgggtggg gttcgtctgt
     2041 acatcgtgca ctgtcagacc cttcctgaag gatttttgtt actgaagtat cagaaggccc
     2101 ttgttctaag gtggtggcac tgtaatatct ggcccaggag gaggtcagtg ccgtgccggc
     2161 gagtcctttg ctcccttcct tctctgacca gtaaacgcat ctccagtcgc tcccccgagg
     2221 cccccctgca cgcagtctcc gacctcagag agtgagtcag atagaagccg ctgcccaccc
     2281 tcccccaccc tggccgggtt cttgcctggc ttgtgcgtcc ttgtgacaat tcctgacacg
     2341 gatgcgcacc gggcagtggc acgtccaggt gctgtgcgcg cccgggccca caaagctcct
     2401 ttggacgctc atggcagccc cttggtggag gaggtcgggt gcccaccccc tccccgccca
     2461 ctgcggaagc cggcgaccca cggagctcgc tctcggccgc cgccacccct ctgtgttcgc
     2521 gcccttccga gctctgatcc gacgctttgt ttcttctcag tgggttcagg gcctgggcca
     2581 gcctttacct acctccccca cccaaaaccg gcaaaagctc agagcacctt gtctgccaaa
     2641 agacagggag ctgggatggt gcgggttggt ctctaaaccg gcgtggggaa aaaagaccct
     2701 ccgtacaaag ccgcagggtg gggctgtcgc aagggcggaa ccgagagggt agctgggggc
     2761 ggggttccca gggccaagag gggccattgt cctccctgga gcccggcgcc cccacagcca
     2821 gctcctctgg gagacagccc ctcctttcga atgcgcgggg ccctcagacc gcgcccggcc
     2881 cagcgctggg ggatccttgg ctgcgggagg ggcgccgcat tgcgcgcggc gggcggggac
     2941 gcgcggtgcg gagcctgcgg gccgggcggg gctctgcggc ggcgcctccc gattggccac
     3001 ccgcggtgac atcagccgat gcgaagggcg gggccgcggc tataagagcg cgcggccgcg
     3061 gtccccgacc ctcagcagcc agcccggccc gcccgcgccc gtccgcagcc gcccgccaga
     3121 cgcgcccagt ggcgcgccat cggatcccgg gcccgtcgaa tcggatcccg ggcccgtcga
     3181 ctgcagaggc ctgcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3241 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3301 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3361 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3421 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3481 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3541 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3601 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3661 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3721 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3781 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3841 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3901 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3961 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4021 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4081 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4141 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4201 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4261 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4321 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4381 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4441 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4501 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4561 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4621 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4681 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4741 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4801 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4861 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4921 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4981 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5041 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5101 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5161 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5221 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5281 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5341 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     5401 aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
