LOCUS       sequence-215971-        6470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa256 of Cre recombinase fused to GID1 CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2211_pCAG-PV1-iCre-N256-L1-GID1-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2522..2542
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2552..2776
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3016..3035)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3081..3136
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3135..3154)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3497..3513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3497..3513)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3510..3532)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3521..3537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3545..3551,3552..3569,3570..3575))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3590..3611
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3696..3830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3850..3984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3887..3906)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3941..3960
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4052..4069)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4223..4811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4303..4322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4982..5773,5774..5842))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5605..5624
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5843..5947)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5978..6357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgt ccaacctgct gactgtgcac caaaacctgc ctgccctccc
      721 tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca
      781 ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg
      841 gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga gggactacct
      901 cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc tgggccagct
      961 caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg ctgtgtccct
     1021 ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca agcaggccct
     1081 ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact ctgacagatg
     1141 ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc tgcgcattgc
     1201 cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga gaatgctgat
     1261 ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg ccctgtccct
     1321 gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg atgaccccaa
     1381 caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctg ccacctccca
     1441 actgtccgga gggtctggct ccggatcaag tggtggcagc ggtaccatgg ccgccagcga
     1501 cgaagtgaac ctgatcgaga gcagaaccgt ggtgcccctg aacacctggg tgctgatctc
     1561 caacttcaag gtggcctaca acatcctgcg gaggcccgac ggcaccttca acagacacct
     1621 ggccgagtac ctggaccgga aagtgaccgc caacgccaac cctgtggacg gcgtgttcag
     1681 cttcgacgtg ctgatcgacc ggcggatcaa cctgctgagc cgggtgtaca gacccgccta
     1741 cgccgatcag gaacagcccc cctctatcct ggatctggaa aagcccgtgg atggcgacat
     1801 cgtgcccgtg atcctgttct tccacggcgg cagctttgcc cacagcagcg ccaatagcgc
     1861 catctacgac accctgtgca gacggctcgt gggcctgtgc aaatgcgtgg tggtgtccgt
     1921 gaactaccgc agagcccccg agaaccctta cccctgcgcc tacgatgatg gctggatcgc
     1981 cctgaactgg gtcaacagca gaagctggct gaagtccaag aaagacagca aggtgcacat
     2041 ctttctggcc ggcgatagca gcggcggcaa tatcgcccat aacgtggccc tgagagccgg
     2101 cgagtctggc atcgatgtgc tgggcaatat cctgctgaac cccatgttcg gcggcaacga
     2161 gcggaccgag agcgagaagt ctctggacgg caagtacttc gtgaccgtgc gggaccggga
     2221 ctggtactgg aaggcctttc tgcccgaggg cgaggacaga gagcaccccg cctgcaatcc
     2281 cttcagcccc agaggcaaaa gcctggaagg cgtgtccttc ccaaagtccc tggtggtggt
     2341 ggccggcctg gacctgatca gagattggca gctggcctat gccgagggcc tgaagaaagc
     2401 cggccaggaa gtgaagctga tgcacctgga aaaggccacc gtgggcttct acctgctgcc
     2461 caacaacaac cacttccaca acgtgatgga cgagatcagc gccttcgtga acgccgagtg
     2521 ccccaagaaa aagcggaagg tgtgagaatt cctgtgcctt ctagttgcca gccatctgtt
     2581 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     2641 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2701 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     2761 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     2821 cacgcgccct gtagcgtgta ccgctgggag ttcgtagacg gaaacaaacg cagaatccaa
     2881 gcgcggccgc ccaggataca tagattacca caactccgag cccttccacc gctagcgcac
     2941 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     3001 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     3061 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     3121 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     3181 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     3241 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     3301 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     3361 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     3421 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     3481 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3541 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3601 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     3661 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccagttc
     3721 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3781 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3841 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3901 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3961 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag
     4021 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4081 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4141 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4201 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4261 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4321 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4381 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4441 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4501 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4561 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4621 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4681 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4741 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4801 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4861 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4921 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4981 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5041 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5101 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5161 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5221 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5281 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5341 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5401 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5461 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5521 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5581 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5641 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5701 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5761 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5821 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5881 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5941 ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat
     6001 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     6061 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     6121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     6181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     6241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     6301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     6361 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     6421 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
//
