LOCUS       sequence-215980-        5407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High Copy number plasmid containing Ple346 (NEFM MiniPromoter) 
            insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2276
FEATURES             Location/Qualifiers
     source          1..5407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3186..3202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3186..3202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3199..3221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3210..3226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3234..3240,3241..3258,3259..3264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3279..3300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3417..3434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3588..4176)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3668..3687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4347..5138,5139..5207))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4970..4989
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5208..5312)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5380..5398
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cggccggccc ttggtatgtt
      421 cttttggact gaaaactgat ggagttggaa gtgacttttg tagtgaaaat gaggctggag
      481 taattaatat tgttccagtg ttctggttat gcgcaggagt gaatcatgat acccaaatta
      541 caatgactca tctcttctga gagaataaat tgtgattcta tgaaagtttt gcttcactag
      601 gaattttttg aagggttctg tttgttctag tttagaaacc tacacaggca atatgggctt
      661 tgcaaaggta taaaattttc ttaaataaaa ctaaacaaag atctattttg attttcagcg
      721 ttggattttt tttttttctt gcctttcctt tgcttctggg agaaagtatc ttcagacagg
      781 aagctttgag ctactcataa attctcccta acactgcaga aacatatttc actatgaaat
      841 ggtggagatg acagtaagag cctcttgttt gaggggcaaa gctgagacat cctgaatgct
      901 atggaagttt aatcttccat gctgacgcaa tcacgggaat attgtgctcc tggggttttc
      961 tttattgcca gctactgtct aatgccagga caaaaggaat aatttgatta cagccgagga
     1021 aagcacagtt tgttcctgtt acttttaaga tgtgcacgca tattaaggtt aacaagattt
     1081 gtggttgtga agtataattc taaagcctca caagcatgaa gggtgtgtgg acgttttcct
     1141 gagtcatcct agggaacaaa agggagtggg cagagctagt taccagagtt ggaaagggat
     1201 gcatcagtca ttacattcag gggctgttag ttggttcctt ttgcaaatgg gctgtgaact
     1261 gcagatgtcc ctgggagaga aaatagaaaa ttactagcaa attcctaaga gaacctggcc
     1321 tctttaaaga agagaagaca cattggggct ggggatgaca tcccaaaact cccagcgccg
     1381 gggagctcag gctcgggcgg tgcctggctc ccctccccgg ttcccgccgc aggaggctgc
     1441 tgagtcactc ccgcctgagc cacggccgcc cccagccagc cacgcgattg gcgggttagc
     1501 ccgggcgcag cgccctcttc ccagctcccg cacccccagg gcttcccgca ggcagagggt
     1561 gggagccccg gcgagcgacc agttggggct tggtaggaac cccctaaaaa tgcgccccac
     1621 ttgtgcattc gtgcatgggc gcattttggt tcagaattaa ttgagagttg ttcactctac
     1681 tatttattaa ctagatgatc cgatagttgc tgcaaagtaa cttgtggtta atgcaggtca
     1741 gtagagagct gatctcactg caggccggag gcaacgccca attcccacgt cttcccctcc
     1801 cccacccaac aagggctggg cagctttaat gcggaacgcc ggtgcagcag cacggagcac
     1861 actcccagac tacagtggcg cccgcactca cgcccttcaa gtgccccacc cctcccacac
     1921 agtggccaag gagccaagcc ctatccctct gcgatctgtc cattgtgcca accctgcatt
     1981 gcagggaagt cttagcctgt gattccagct tcgctcctgc aaagatagtc actacaggca
     2041 ggtttaaatt gtatttactc ggagagaaga gggggaaggg aaccccctgc cccctgccaa
     2101 cctgacaggc caggctgggt gacaagcccc ccgggtggct gcacctccca ctcgccgacc
     2161 tgggcgctgt ccttggtgct gccggcgcca tagcgagcac aagcgcctta ccttctgccc
     2221 ctgcggaagc tgcggacgtc ccccaaacgt taaaaacgcc atatcccact ttttatacaa
     2281 ataactaccc cggcctgttc tctttgtccc cttcccgaag ccttcaggca gaccgttccg
     2341 caaagtcttt ttttttgttt tgttttgtct tctagaccca gctcctcggc gctcatctcc
     2401 agctgcttta agacaagggg tgggggaagg ggagggaggc aagaaaagat gagggtgggg
     2461 gaggggaaaa gagggaatgc aaggggaagg agggaggaga cggggagaag gaaagattgg
     2521 aagaaaagga tctccgagga aggggctgag agaagggcag ggtgaactgg actaaaggcc
     2581 agagtaggaa ggagaagagg ggccaaaaaa gaaggggatg aaattaagca cagaagatgg
     2641 gtaaagaaaa aagtatcagg gaaagggcaa aataagagaa agccttgagg ataagagggt
     2701 agaaggctaa agaacaaggg gaccactggg tcggggaagc gctgcctgaa cggcgggaca
     2761 gtgacaaaga aagggcgctg gcgatattcg caccaagggt gcgaaacgca atcgggaggt
     2821 gagaaatgga aagaaggcga atgcccggct acaagtagcc tgggactgaa aggggacctg
     2881 ggggaggggc tgggcccagg gcagaaaagt ccaggttccc atgcggcctg ggcccacgtg
     2941 gagcgggcgc tgaatcaccg ttcagccgcc cccctcccct cctccccgac cggtgcccgc
     3001 agtccccgcc tcctcggccg ccgcctccac ggggcggggc cctggcccgg gaccagcgcc
     3061 gcggctataa atgggctgcg gcgaggccgg cagaacgctg tgacagccac acgccccaag
     3121 ggcgcgccat cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa
     3181 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3241 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3301 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3361 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3421 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3481 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3541 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3601 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3661 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3721 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3781 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3841 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3901 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3961 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4021 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4081 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4141 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4201 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4261 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4321 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4381 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4441 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4501 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4561 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4621 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4681 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4741 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4801 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4861 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4921 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4981 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5041 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5101 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5161 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5221 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5281 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5341 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     5401 tttcgtc
//
