LOCUS       sequence-216000-        8839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of 
            plasmid pMM643, ColE1 origin, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins
            J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, 
            Traverso G, Chandrakasan AP, Lu TK
  TITLE     An ingestible bacterial-electronic system to monitor 
            gastrointestinal health.
  JOURNAL   Science. 2018 May 25;360(6391):915-918. doi: 
            10.1126/science.aas9315.
  PUBMED    29798884
REFERENCE   2  (bases 1 to 8839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-25- 25"; volume: "360"; 
            issue: "6391"; pages: "915-918"
COMMENT     Sequence Label: pMM1157
FEATURES             Location/Qualifiers
     source          1..8839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      635..721
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(901..1489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(981..1000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1577..1671)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1702..2496)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1813..1832)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2423..2442
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2675..2693
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             2796..2807
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2814..4256
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             4269..5192
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             5241..6323
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             6338..7321
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             7500..8612
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
ORIGIN
        1 tatactctaa ttaatcacat aataaggacg aatttatgcc aaaatcaacc tattttagtc
       61 tttctgacga aaaacgaaaa cgtgtctatg atgcctgttt actagaattt caaacgcact
      121 ctttccatga agctaaaatc atgcacatcg taaaagcact tgatatccca agaggaagtt
      181 tttatcaata ctttgaagat ttgaaggatt catactatta tatcttgtca caggaaactg
      241 tcgagattca tgatttattt tttaatttac taaaagaata tcctctagaa gttgctctta
      301 ataaatacaa gtatcttctt cttgaaaatt tagtaaattc gccccaatat aatctttata
      361 aatatcgatt tttagattgg acttatgaat tagaaagaga ttggaagcct aaaggcgagg
      421 taactgttcc cgctcgtgaa cttgataatc ctatttccca agtattaaaa tcagtcattc
      481 acaatctagt ttatcgcatg tttagtgaaa attgggatga acaaaagttt attgaaactt
      541 acgataaaga aatcaaattg ctcacagagg gcttgcttaa ttatgttact gaaagcaaaa
      601 aatagggatc ccatggtacg cgtgctagag gcatcaaata aaacgaaagg ctcagtcgaa
      661 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
      721 tccgccgccc tagaggaaaa cctaggtaga cctaggcgtt cggctgcggc gagcggtatc
      781 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      841 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      901 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      961 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1021 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1081 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1141 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1201 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1261 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1321 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     1381 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1441 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1501 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1561 catgactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     1621 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     1681 tctcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     1741 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     1801 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     1861 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     1921 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
     1981 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     2041 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     2101 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     2161 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     2221 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     2281 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     2341 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     2401 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     2461 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     2521 ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     2581 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccgacgtcta
     2641 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     2701 tcttcacctc gagatgctat aaatgacaca gtgtcatttg acaaaatgac acagtgtcat
     2761 gatactgagc acatcagcag gacgcactga ccattaaaga ggagaaaggt accatgacta
     2821 aaaaaatttc attcattatt aacggccagg ttgaaatctt tcccgaaggt gatgatttag
     2881 tgcaatccat taattttggt gataatagtg tttacctgcc aatattgaat gactctcatg
     2941 taaaaaacat tattgattgt aatggaaata acgaattacg gttgcataac attgtcaatt
     3001 ttctctatac ggtagggcaa agatggaaaa atgaagaata ctcaagacgc aggacataca
     3061 ttcgtgactt aaaaaaatat atgggatatt cagaagaaat ggctaagcta gaggccaatt
     3121 ggatatctat gattttatgt tctaaaggcg gcctttatga tgttgtagaa aatgaacttg
     3181 gttctcgcca tatcatggat gaatggctac ctcaggatga aagttatgtt cgggcttttc
     3241 cgaaaggtaa atctgtacat ctgttggcag gtaatgttcc attatctggg atcatgtcta
     3301 tattacgcgc aattttaact aagaatcagt gtattataaa aacatcgtca accgatcctt
     3361 ttaccgctaa tgcattagcg ttaagtttta ttgatgtaga ccctaatcat ccgataacgc
     3421 gctctttatc tgttatatat tggccccacc aaggtgatac atcactcgca aaagaaatta
     3481 tgcaacatgc ggatgttatt gtcgcttggg gagggccaga tgcgattaat tgggcggtag
     3541 agcatgcgcc atcttatgct gatgtgatta aatttggttc taaaaagagt ctttgcatta
     3601 tcgataatcc tgttgatttg acgtccgcag cgacaggtgc ggctcatgat gtttgttttt
     3661 acgatcagcg agcttgtttt tctgcccaaa acatatatta catgggaaat cattatgagg
     3721 aatttaagtt agcgttgata gaaaaactta atctatatgc gcatatatta ccgaatgcca
     3781 aaaaagattt tgatgaaaag gcggcctatt ctttagttca aaaagaaagc ttgtttgctg
     3841 gattaaaagt agaggtggat attcatcaac gttggatgat tattgagtca aatgcaggtg
     3901 tggaatttaa tcaaccactt ggcagatgtg tgtaccttca tcacgtcgat aatattgagc
     3961 aaatattgcc ttatgttcaa aaaaataaga cgcaaaccat atctattttt ccttgggagt
     4021 catcatttaa atatcgagat gcgttagcat taaaaggtgc ggaaaggatt gtagaagcag
     4081 gaatgaataa catatttcga gttggtggat ctcatgacgg aatgcgaccg ttgcaacgat
     4141 tagtgacata tatttctcat gaaaggccat ctaactatac ggctaaggat gttgcggttg
     4201 aaatagaaca gactcgattc ctggaagaag ataagttcct tgtatttgtc ccataatagg
     4261 taaaaagtat ggaaaatgaa tcaaaatata aaaccatcga ccacgttatt tgtgttgaag
     4321 gaaataaaaa aattcatgtt tgggaaacgc tgccagaaga aaacagccca aagagaaaga
     4381 atgccattat tattgcgtct ggttttgccc gcaggatgga tcattttgct ggtctggcgg
     4441 aatatttatc gcggaatgga tttcatgtga tccgctatga ttcgcttcac cacgttggat
     4501 tgagttcagg gacaattgat gaatttacaa tgtctatagg aaagcagagc ttgttagcag
     4561 tggttgattg gttaactaca cgaaaaataa ataacttcgg tatgttggct tcaagcttat
     4621 ctgcgcggat agcttatgca agcctatctg aaatcaatgc ttcgttttta atcaccgcag
     4681 tcggtgttgt taacttaaga tattctcttg aaagagcttt agggtttgat tatctcagtc
     4741 tacccattaa tgaattgccg aataatctag attttgaagg ccataaattg ggtgctgaag
     4801 tctttgcgag agattgtctt gattttggtt gggaagattt agcttctaca attaataaca
     4861 tgatgtatct tgatataccg tttattgctt ttactgcaaa taacgataat tgggtcaagc
     4921 aagatgaagt tatcacattg ttatcaaata ttcgtagtaa tcgatgcaag atatattctt
     4981 tgttaggaag ttcgcatgac ttgagtgaaa atttagtggt cctgcgcaat ttttatcaat
     5041 cggttacgaa agccgctatc gcgatggata atgatcatct ggatattgat gttgatatta
     5101 ctgaaccgtc atttgaacat ttaactattg cgacagtcaa tgaacgccga atgagaattg
     5161 agattgaaaa tcaagcaatt tctctgtctt aaaatctatt gagatattct atcactcaaa
     5221 tagcaatata aggactctct atgaaatttg gaaacttttt gcttacatac caacctcccc
     5281 aattttctca aacagaggta atgaaacgtt tggttaaatt aggtcgcatc tctgaggagt
     5341 gtggttttga taccgtatgg ttactggagc atcatttcac ggagtttggt ttgcttggta
     5401 acccttatgt cgctgctgca tatttacttg gcgcgactaa aaaattgaat gtaggaactg
     5461 ccgctattgt tcttcccaca gcccatccag tacgccaact tgaagatgtg aatttattgg
     5521 atcaaatgtc aaaaggacga tttcggtttg gtatttgccg agggctttac aacaaggact
     5581 ttcgcgtatt cggcacagat atgaataaca gtcgcgcctt agcggaatgc tggtacgggc
     5641 tgataaagaa tggcatgaca gagggatata tggaagctga taatgaacat atcaagttcc
     5701 ataaggtaaa agtaaacccc gcggcgtata gcagaggtgg cgcaccggtt tatgtggtgg
     5761 ctgaatcagc ttcgacgact gagtgggctg ctcaatttgg cctaccgatg atattaagtt
     5821 ggattataaa tactaacgaa aagaaagcac aacttgagct ttataatgaa gtggctcaag
     5881 aatatgggca cgatattcat aatatcgacc attgcttatc atatataaca tctgtagatc
     5941 atgactcaat taaagcgaaa gagatttgcc ggaaatttct ggggcattgg tatgattctt
     6001 atgtgaatgc tacgactatt tttgatgatt cagaccaaac aagaggttat gatttcaata
     6061 aagggcagtg gcgtgacttt gtattaaaag gacataaaga tactaatcgc cgtattgatt
     6121 acagttacga aatcaatccc gtgggaacgc cgcaggaatg tattgacata attcaaaaag
     6181 acattgatgc tacaggaata tcaaatattt gttgtggatt tgaagctaat ggaacagtag
     6241 acgaaattat tgcttccatg aagctcttcc agtctgatgt catgccattt cttaaagaaa
     6301 aacaacgttc gctattatat tagctaagga gaaagaaatg aaatttggat tgttcttcct
     6361 taacttcatc aattcaacaa ctgttcaaga acaaagtata gttcgcatgc aggaaataac
     6421 ggagtatgtt gataagttga attttgaaca gattttagtg tatgaaaatc atttttcaga
     6481 taatggtgtt gtcggcgctc ctctgactgt ttctggtttt ctgctcggtt taacagagaa
     6541 aattaaaatt ggttcattaa atcacatcat tacaactcat catcctgtcc gcatagcgga
     6601 ggaagcttgc ttattggatc agttaagtga agggagattt attttagggt ttagtgattg
     6661 cgaaaaaaaa gatgaaatgc atttttttaa tcgcccggtt gaatatcaac agcaactatt
     6721 tgaagagtgt tatgaaatca ttaacgatgc tttaacaaca ggctattgta atccagataa
     6781 cgatttttat agcttcccta aaatatctgt aaatccccat gcttatacgc caggcggacc
     6841 tcggaaatat gtaacagcaa ccagtcatca tattgttgag tgggcggcca aaaaaggtat
     6901 tcctctcatc tttaagtggg atgattctaa tgatgttaga tatgaatatg ctgaaagata
     6961 taaagccgtt gcggataaat atgacgttga cctatcagag atagaccatc agttaatgat
     7021 attagttaac tataacgaag atagtaataa agctaaacaa gagacgcgtg catttattag
     7081 tgattatgtt cttgaaatgc accctaatga aaatttcgaa aataaacttg aagaaataat
     7141 tgcagaaaac gctgtcggaa attatacgga gtgtataact gcggctaagt tggcaattga
     7201 aaagtgtggt gcgaaaagtg tattgctgtc ctttgaacca atgaatgatt tgatgagcca
     7261 aaaaaatgta atcaatattg ttgatgataa tattaagaag taccacatgg aatataccta
     7321 atagatttcg agttgcagcg aggcggcaag tgaacgaatc cccaggagca tagataacta
     7381 tgtgactggg gtgagtgaaa gcagccaaca aagcagcagc ttgaaagatg aagggtataa
     7441 aagagtatga cagcagtgct gccatacttt ctaatattat cttgaggagt aaaacaggta
     7501 tgacttcata tgttgataaa caagaaatta cagcaagctc agaaattgat gatttgattt
     7561 tttcgagcga tccattagtg tggtcttacg acgagcagga aaaaatcaga aagaaacttg
     7621 tgcttgatgc atttcgtaat cattataaac attgtcgaga atatcgtcac tactgtcagg
     7681 cacacaaagt agatgacaat attacggaaa ttgatgacat acctgtattc ccaacatcgg
     7741 tttttaagtt tactcgctta ttaacttctc aggaaaacga gattgaaagt tggtttacca
     7801 gtagcggcac gaatggttta aaaagtcagg tggcgcgtga cagattaagt attgagagac
     7861 tcttaggctc tgtgagttat ggcatgaaat atgttggtag ttggtttgat catcaaatag
     7921 aattagtcaa tttgggacca gatagattta atgctcataa tatttggttt aaatatgtta
     7981 tgagtttggt ggaattgtta tatcctacga catttaccgt aacagaagaa cgaatagatt
     8041 ttgttaaaac attgaatagt cttgaacgaa taaaaaatca agggaaagat ctttgtctta
     8101 ttggttcgcc atactttatt tatttactct gccattatat gaaagataaa aaaatctcat
     8161 tttctggaga taaaagcctt tatatcataa ccggaggcgg ctggaaaagt tacgaaaaag
     8221 aatctctgaa acgtgatgat ttcaatcatc ttttatttga tactttcaat ctcagtgata
     8281 ttagtcagat ccgagatata tttaatcaag ttgaactcaa cacttgtttc tttgaggatg
     8341 aaatgcagcg taaacatgtt ccgccgtggg tatatgcgcg agcgcttgat cctgaaacgt
     8401 tgaaacctgt acctgatgga acgccggggt tgatgagtta tatggatgcg tcagcaacca
     8461 gttatccagc atttattgtt accgatgatg tcgggataat tagcagagaa tatggtaagt
     8521 atcccggcgt gctcgttgaa attttacgtc gcgtcaatac gaggacgcag aaagggtgtg
     8581 ctttaagctt aaccgaagcg tttgatagtt gaaagcttga tatcgaattc ctgcccaatt
     8641 attgaagggg agcgggaaac cgctcccctt tttttgtttc tggtctcccc acagctaaca
     8701 ccacgtcgtc cctatctgct gccctaggtc tatgagtggt tgctggataa ctttacgggc
     8761 atgcataagg ctcgtataat atattcaggg agaccacaac ggtttccctc tacaaataat
     8821 tttgtttaac tttgaattc
//
