LOCUS       sequence-216001-       10947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 
            (Strength 33545.5 AU), ColE1 origin, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins
            J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, 
            Traverso G, Chandrakasan AP, Lu TK
  TITLE     An ingestible bacterial-electronic system to monitor 
            gastrointestinal health.
  JOURNAL   Science. 2018 May 25;360(6391):915-918. doi: 
            10.1126/science.aas9315.
  PUBMED    29798884
REFERENCE   2  (bases 1 to 10947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2018-05-25- 25"; volume: "360"; 
            issue: "6391"; pages: "915-918"
COMMENT     Sequence Label: pMM643
FEATURES             Location/Qualifiers
     source          1..10947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      635..721
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        complement(2814..2848)
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     rep_origin      complement(3009..3597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3089..3108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3685..3779)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3810..4604)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3921..3940)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4531..4550
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4783..4801
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             4904..4915
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4922..6364
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             6377..7300
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             7349..8431
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             8446..9429
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             9608..10720
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
ORIGIN
        1 tatactctaa ttaatcacat aataaggacg aatttatgcc aaaatcaacc tattttagtc
       61 tttctgacga aaaacgaaaa cgtgtctatg atgcctgttt actagaattt caaacgcact
      121 ctttccatga agctaaaatc atgcacatcg taaaagcact tgatatccca agaggaagtt
      181 tttatcaata ctttgaagat ttgaaggatt catactatta tatcttgtca caggaaactg
      241 tcgagattca tgatttattt tttaatttac taaaagaata tcctctagaa gttgctctta
      301 ataaatacaa gtatcttctt cttgaaaatt tagtaaattc gccccaatat aatctttata
      361 aatatcgatt tttagattgg acttatgaat tagaaagaga ttggaagcct aaaggcgagg
      421 taactgttcc cgctcgtgaa cttgataatc ctatttccca agtattaaaa tcagtcattc
      481 acaatctagt ttatcgcatg tttagtgaaa attgggatga acaaaagttt attgaaactt
      541 acgataaaga aatcaaattg ctcacagagg gcttgcttaa ttatgttact gaaagcaaaa
      601 aatagggatc ccatggtacg cgtgctagag gcatcaaata aaacgaaagg ctcagtcgaa
      661 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
      721 tccgccgccc tagaggacca aaacgaaaaa aggcccccct ttcgggaggc ctcttttctg
      781 aaatttggta ccgagttacc attgataact cacgaaaatt tttccgttac gaccatcctg
      841 tgggatgcct tgcggcgacc agtactcttt gtcgaaagcg ttacccaaca ccaaagtagt
      901 ggtcatacct ttgagcgcct gttgtccttg ataactgacg tagaaatcat tcacgccata
      961 gcctggttgt ttgctgtaac tgctgctgat atgtgttgag cgatcggcaa acgtaccaac
     1021 ccacccaaca gagaagccac tgtgagcgat cggaatattc agagtgctgg taacagtatc
     1081 cgggttaatg ctggagatgt attcgccggt atcggtgtct ttgccgcggg tacggttata
     1141 ggccacatca aggctaaaca gatcagtggt atatttcgtc atcacatccc agccccagat
     1201 tttggcgttc gggacgttat acgacatagt cgtcgccgcc gcgaaatcga cggtcgtgga
     1261 gatgtaatcc ttcgctttgg tatcaaagta gctggcttta aattccagag catcattgga
     1321 caacatcagg tcatcaaaac gcagcccaaa accgtactcc tgagtttcgt tagtttccgg
     1381 acgtaagttc gggtttggca cccaatagtt ggtatagaag cgaccaatcg agaagtgctt
     1441 agaatcgtta tacatttcgc ccatcgtcgg ggcgcggaat gcctgggcat atgagccaaa
     1501 taacatcagc cagttagtcg gattgatagt catccccgca cgagatgacc atttgtcggc
     1561 atcaacgtct ttgtaaccgt cactgctacc gcgataactg tcatagcggg ttccgccaag
     1621 cagggtaatc ggcagatcgc gtaaggtgat ctcatcctgt agccagccgg agctaaaatc
     1681 gatttttgct tgcgggaagc ccgtcgtcgc gccgcccgga tgttgttcct gacgataata
     1741 ctcaccgcca tatgtcagta agtgagaagc gaaactgtcg gcaaagagag tggaacggtt
     1801 ctccagcctg gctccttttg ttatctgttc gcgatactcg ccggaactcc ccgtgttttg
     1861 cgcattaata cggacttccg accaataaat ttttgcatct gcatttaacc agtcgttgcc
     1921 ctgcggggcg agtttataag aaagctgcgc atcgcgttga attgttgaac gatcaaccat
     1981 cgggttgctg ctttcagaag ccccaacggt ctgcggattt tttggttcac gcgcgtcgtt
     2041 gttgtagtaa cgcactaaac cgctcagaga ctgggctgaa tcaatttgcc aggtcccttt
     2101 cgccagcatg ttattaatgg actcgtcatt cggcgcggtt tcaccattgc tctggcgtaa
     2161 atcaccccga tcgcgactgg accaggccac aataccatcc agattttcag ttcgcccaaa
     2221 cgcgctcgcg cctaatccca ggctatggtc ccccgtgccg ccagtaccaa agacacgaaa
     2281 accactgctt tgtccttcct gcaataaatc ttttgcatcg accgtatcgt aggagatcac
     2341 tccacccagc gcgccactgc catacagtaa tgctgaaggt ccacgaacaa tctcaacacg
     2401 cttgatcagc gccggatcga gaaaagtgcc attcaggtgt ccggtatccg ttccctgacg
     2461 aacaccatcg acaagaacca gcacgccgcg atgatcatag ccacgcatat ttacatcctg
     2521 accgttggtt cgtccggtac catccagagt aattccagga acatgacgca gcagatcggt
     2581 ggctgaagta gccgtttgat tttcaggagc ggaagtgtcg atgacgctga ccatcatagg
     2641 cgcttcgaag gaactacggg cattccccgt tgccgtaacg gtcatggttt cagtagcaaa
     2701 agcaaacgtt ggcaaggtgg cagaaacagg taaggccaat aaactcaaac gcaacgaggt
     2761 aaattgcgga cgtgacatga tttaaagctc ccttgggtta gtgttggaaa tgtgctagca
     2821 taatacctag ggctgagcta gccgtaaacc taggtagacc taggcgttcg gctgcggcga
     2881 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2941 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3001 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3061 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3181 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3301 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3361 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3421 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3601 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3661 attttggtca tgactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     3721 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     3781 aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     3841 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     3901 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     3961 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     4021 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc
     4081 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     4141 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     4201 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     4261 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     4321 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     4381 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca
     4441 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     4501 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     4561 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     4621 cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     4681 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     4741 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     4801 ccctttcgtc ttcacctcga gatgctataa atgacacagt gtcatttgac aaaatgacac
     4861 agtgtcatga tactgagcac atcagcagga cgcactgacc attaaagagg agaaaggtac
     4921 catgactaaa aaaatttcat tcattattaa cggccaggtt gaaatctttc ccgaaggtga
     4981 tgatttagtg caatccatta attttggtga taatagtgtt tacctgccaa tattgaatga
     5041 ctctcatgta aaaaacatta ttgattgtaa tggaaataac gaattacggt tgcataacat
     5101 tgtcaatttt ctctatacgg tagggcaaag atggaaaaat gaagaatact caagacgcag
     5161 gacatacatt cgtgacttaa aaaaatatat gggatattca gaagaaatgg ctaagctaga
     5221 ggccaattgg atatctatga ttttatgttc taaaggcggc ctttatgatg ttgtagaaaa
     5281 tgaacttggt tctcgccata tcatggatga atggctacct caggatgaaa gttatgttcg
     5341 ggcttttccg aaaggtaaat ctgtacatct gttggcaggt aatgttccat tatctgggat
     5401 catgtctata ttacgcgcaa ttttaactaa gaatcagtgt attataaaaa catcgtcaac
     5461 cgatcctttt accgctaatg cattagcgtt aagttttatt gatgtagacc ctaatcatcc
     5521 gataacgcgc tctttatctg ttatatattg gccccaccaa ggtgatacat cactcgcaaa
     5581 agaaattatg caacatgcgg atgttattgt cgcttgggga gggccagatg cgattaattg
     5641 ggcggtagag catgcgccat cttatgctga tgtgattaaa tttggttcta aaaagagtct
     5701 ttgcattatc gataatcctg ttgatttgac gtccgcagcg acaggtgcgg ctcatgatgt
     5761 ttgtttttac gatcagcgag cttgtttttc tgcccaaaac atatattaca tgggaaatca
     5821 ttatgaggaa tttaagttag cgttgataga aaaacttaat ctatatgcgc atatattacc
     5881 gaatgccaaa aaagattttg atgaaaaggc ggcctattct ttagttcaaa aagaaagctt
     5941 gtttgctgga ttaaaagtag aggtggatat tcatcaacgt tggatgatta ttgagtcaaa
     6001 tgcaggtgtg gaatttaatc aaccacttgg cagatgtgtg taccttcatc acgtcgataa
     6061 tattgagcaa atattgcctt atgttcaaaa aaataagacg caaaccatat ctatttttcc
     6121 ttgggagtca tcatttaaat atcgagatgc gttagcatta aaaggtgcgg aaaggattgt
     6181 agaagcagga atgaataaca tatttcgagt tggtggatct catgacggaa tgcgaccgtt
     6241 gcaacgatta gtgacatata tttctcatga aaggccatct aactatacgg ctaaggatgt
     6301 tgcggttgaa atagaacaga ctcgattcct ggaagaagat aagttccttg tatttgtccc
     6361 ataataggta aaaagtatgg aaaatgaatc aaaatataaa accatcgacc acgttatttg
     6421 tgttgaagga aataaaaaaa ttcatgtttg ggaaacgctg ccagaagaaa acagcccaaa
     6481 gagaaagaat gccattatta ttgcgtctgg ttttgcccgc aggatggatc attttgctgg
     6541 tctggcggaa tatttatcgc ggaatggatt tcatgtgatc cgctatgatt cgcttcacca
     6601 cgttggattg agttcaggga caattgatga atttacaatg tctataggaa agcagagctt
     6661 gttagcagtg gttgattggt taactacacg aaaaataaat aacttcggta tgttggcttc
     6721 aagcttatct gcgcggatag cttatgcaag cctatctgaa atcaatgctt cgtttttaat
     6781 caccgcagtc ggtgttgtta acttaagata ttctcttgaa agagctttag ggtttgatta
     6841 tctcagtcta cccattaatg aattgccgaa taatctagat tttgaaggcc ataaattggg
     6901 tgctgaagtc tttgcgagag attgtcttga ttttggttgg gaagatttag cttctacaat
     6961 taataacatg atgtatcttg atataccgtt tattgctttt actgcaaata acgataattg
     7021 ggtcaagcaa gatgaagtta tcacattgtt atcaaatatt cgtagtaatc gatgcaagat
     7081 atattctttg ttaggaagtt cgcatgactt gagtgaaaat ttagtggtcc tgcgcaattt
     7141 ttatcaatcg gttacgaaag ccgctatcgc gatggataat gatcatctgg atattgatgt
     7201 tgatattact gaaccgtcat ttgaacattt aactattgcg acagtcaatg aacgccgaat
     7261 gagaattgag attgaaaatc aagcaatttc tctgtcttaa aatctattga gatattctat
     7321 cactcaaata gcaatataag gactctctat gaaatttgga aactttttgc ttacatacca
     7381 acctccccaa ttttctcaaa cagaggtaat gaaacgtttg gttaaattag gtcgcatctc
     7441 tgaggagtgt ggttttgata ccgtatggtt actggagcat catttcacgg agtttggttt
     7501 gcttggtaac ccttatgtcg ctgctgcata tttacttggc gcgactaaaa aattgaatgt
     7561 aggaactgcc gctattgttc ttcccacagc ccatccagta cgccaacttg aagatgtgaa
     7621 tttattggat caaatgtcaa aaggacgatt tcggtttggt atttgccgag ggctttacaa
     7681 caaggacttt cgcgtattcg gcacagatat gaataacagt cgcgccttag cggaatgctg
     7741 gtacgggctg ataaagaatg gcatgacaga gggatatatg gaagctgata atgaacatat
     7801 caagttccat aaggtaaaag taaaccccgc ggcgtatagc agaggtggcg caccggttta
     7861 tgtggtggct gaatcagctt cgacgactga gtgggctgct caatttggcc taccgatgat
     7921 attaagttgg attataaata ctaacgaaaa gaaagcacaa cttgagcttt ataatgaagt
     7981 ggctcaagaa tatgggcacg atattcataa tatcgaccat tgcttatcat atataacatc
     8041 tgtagatcat gactcaatta aagcgaaaga gatttgccgg aaatttctgg ggcattggta
     8101 tgattcttat gtgaatgcta cgactatttt tgatgattca gaccaaacaa gaggttatga
     8161 tttcaataaa gggcagtggc gtgactttgt attaaaagga cataaagata ctaatcgccg
     8221 tattgattac agttacgaaa tcaatcccgt gggaacgccg caggaatgta ttgacataat
     8281 tcaaaaagac attgatgcta caggaatatc aaatatttgt tgtggatttg aagctaatgg
     8341 aacagtagac gaaattattg cttccatgaa gctcttccag tctgatgtca tgccatttct
     8401 taaagaaaaa caacgttcgc tattatatta gctaaggaga aagaaatgaa atttggattg
     8461 ttcttcctta acttcatcaa ttcaacaact gttcaagaac aaagtatagt tcgcatgcag
     8521 gaaataacgg agtatgttga taagttgaat tttgaacaga ttttagtgta tgaaaatcat
     8581 ttttcagata atggtgttgt cggcgctcct ctgactgttt ctggttttct gctcggttta
     8641 acagagaaaa ttaaaattgg ttcattaaat cacatcatta caactcatca tcctgtccgc
     8701 atagcggagg aagcttgctt attggatcag ttaagtgaag ggagatttat tttagggttt
     8761 agtgattgcg aaaaaaaaga tgaaatgcat ttttttaatc gcccggttga atatcaacag
     8821 caactatttg aagagtgtta tgaaatcatt aacgatgctt taacaacagg ctattgtaat
     8881 ccagataacg atttttatag cttccctaaa atatctgtaa atccccatgc ttatacgcca
     8941 ggcggacctc ggaaatatgt aacagcaacc agtcatcata ttgttgagtg ggcggccaaa
     9001 aaaggtattc ctctcatctt taagtgggat gattctaatg atgttagata tgaatatgct
     9061 gaaagatata aagccgttgc ggataaatat gacgttgacc tatcagagat agaccatcag
     9121 ttaatgatat tagttaacta taacgaagat agtaataaag ctaaacaaga gacgcgtgca
     9181 tttattagtg attatgttct tgaaatgcac cctaatgaaa atttcgaaaa taaacttgaa
     9241 gaaataattg cagaaaacgc tgtcggaaat tatacggagt gtataactgc ggctaagttg
     9301 gcaattgaaa agtgtggtgc gaaaagtgta ttgctgtcct ttgaaccaat gaatgatttg
     9361 atgagccaaa aaaatgtaat caatattgtt gatgataata ttaagaagta ccacatggaa
     9421 tatacctaat agatttcgag ttgcagcgag gcggcaagtg aacgaatccc caggagcata
     9481 gataactatg tgactggggt gagtgaaagc agccaacaaa gcagcagctt gaaagatgaa
     9541 gggtataaaa gagtatgaca gcagtgctgc catactttct aatattatct tgaggagtaa
     9601 aacaggtatg acttcatatg ttgataaaca agaaattaca gcaagctcag aaattgatga
     9661 tttgattttt tcgagcgatc cattagtgtg gtcttacgac gagcaggaaa aaatcagaaa
     9721 gaaacttgtg cttgatgcat ttcgtaatca ttataaacat tgtcgagaat atcgtcacta
     9781 ctgtcaggca cacaaagtag atgacaatat tacggaaatt gatgacatac ctgtattccc
     9841 aacatcggtt tttaagttta ctcgcttatt aacttctcag gaaaacgaga ttgaaagttg
     9901 gtttaccagt agcggcacga atggtttaaa aagtcaggtg gcgcgtgaca gattaagtat
     9961 tgagagactc ttaggctctg tgagttatgg catgaaatat gttggtagtt ggtttgatca
    10021 tcaaatagaa ttagtcaatt tgggaccaga tagatttaat gctcataata tttggtttaa
    10081 atatgttatg agtttggtgg aattgttata tcctacgaca tttaccgtaa cagaagaacg
    10141 aatagatttt gttaaaacat tgaatagtct tgaacgaata aaaaatcaag ggaaagatct
    10201 ttgtcttatt ggttcgccat actttattta tttactctgc cattatatga aagataaaaa
    10261 aatctcattt tctggagata aaagccttta tatcataacc ggaggcggct ggaaaagtta
    10321 cgaaaaagaa tctctgaaac gtgatgattt caatcatctt ttatttgata ctttcaatct
    10381 cagtgatatt agtcagatcc gagatatatt taatcaagtt gaactcaaca cttgtttctt
    10441 tgaggatgaa atgcagcgta aacatgttcc gccgtgggta tatgcgcgag cgcttgatcc
    10501 tgaaacgttg aaacctgtac ctgatggaac gccggggttg atgagttata tggatgcgtc
    10561 agcaaccagt tatccagcat ttattgttac cgatgatgtc gggataatta gcagagaata
    10621 tggtaagtat cccggcgtgc tcgttgaaat tttacgtcgc gtcaatacga ggacgcagaa
    10681 agggtgtgct ttaagcttaa ccgaagcgtt tgatagttga aagcttgata tcgaattcct
    10741 gcccaattat tgaaggggag cgggaaaccg ctcccctttt tttgtttctg gtctccccac
    10801 agctaacacc acgtcgtccc tatctgctgc cctaggtcta tgagtggttg ctggataact
    10861 ttacgggcat gcataaggct cgtataatat attcagggag accacaacgg tttccctcta
    10921 caaataattt tgtttaactt tgaattc
//
