LOCUS       sequence-216017-       11659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of N-terminally tagged 
            eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged
            chromatin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling 
            AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato
            S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, 
            Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY,
            Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, 
            Lord CJ, Rottenberg S, Durocher D
  TITLE     The shieldin complex mediates 53BP1-dependent DNA repair.
  JOURNAL   Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. 
            Epub 2018 Jul 18.
  PUBMED    30022168
REFERENCE   2  (bases 1 to 11659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0340-7"; journalName: "Nature"; date: "2018-08";
            volume: "560"; issue: "7716"; pages: "117-121"
COMMENT     Sequence Label: pCW-eGFP-FHA-SHLD2
FEATURES             Location/Qualifiers
     source          1..11659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        214..528
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    258..276
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    293..311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    329..347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    365..383
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    400..418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    436..454
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     501..525
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    549..573
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(591..593,594..596,597..1307)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(591..593,594..596,597..1307)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(636..657)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(897..916)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1244..1265
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4534..4558)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4576..4593
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4603..5113
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5032..5050
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5123..5719
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5123..5142)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5619..5639
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5720..5773
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5774..6391,6392..6397,6398..6433,6434..6436,6437..6472,
                     6473..6475,6476..6511,6512..6520)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6560..6580)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6978..6989)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7194..7427
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7499..7620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7536..7555)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7590..7609
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7660..7795
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7795..7866
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7867..7935,7936..8727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8085..8104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8898..9486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9387..9406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9640..9657
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9774..9795
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9810..9815,9816..9833,9834..9840)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9848..9864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9853..9875
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9872..9888
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9872..9888
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9907..9927
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9909..9927
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9955..10181
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10182..10362
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10409..10534
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11027..11260
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11445..11489
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca ctttggccgc gaatcgatat gtcgagttta ctccctatca gtgatagaga
      241 acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc
      301 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      361 ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac
      421 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
      481 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattggc
      541 tagcatcaac aagtttgtac aaaaaagcag gcttcgaagg agatagaacc atggtgagca
      601 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      661 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      721 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      781 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      841 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      901 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      961 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1021 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1081 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1141 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1201 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1261 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc gcgccagccg
     1321 gagccgggga gcccggcttc ttcgtcacag gagaccgcgc cggtggccgg agctggtgcc
     1381 tgcggcgggt ggggatgagc gccgggtggc tgctgctgga agatgggtgc gaggtgactg
     1441 taggacgagg atttggtgtc acataccaac tggtatcaaa aatctgcccc ctgatgattt
     1501 ctcgaaacca ctgtgttttg aagcagaatc ctgagggcca atggacaatt atggacaaca
     1561 agagtctaaa tggtgtttgg ctgaacagag cgcgtctgga acctttaagg gtctattcca
     1621 ttcatcaggg agactacatc caacttggag tgcctctgga aaataaggag aatgcggagt
     1681 atgaatatga agttactgaa gaagactggg agacaatata tccttgtctt tccccaaaga
     1741 atgaccaaat gatagaaaaa aataaggaat tgagaactaa aaggaaattc agtttggatg
     1801 aagccggagc cggcgcgcca agtggaggat ctcaagtcca cattttttgg ggtgctccaa
     1861 ttgctccact gaaaatcaca gtatcagaag acacagcttc tttaatgtct gttgctgacc
     1921 cctggaaaaa aattcagctt ttatacagtc aacattcttt atatctgaag gatgaaaaac
     1981 agcacaaaaa tcttgaaaac tataaagtcc cagaatctat tggttctcca gatcttagtg
     2041 gtcatttctt agcaaactgt atgaatagac atgttcatgt gaaagatgac tttgtacgtt
     2101 ctgtttctga aacacagaat atagaatccc agaagattca ctcctctaga ctgagtgata
     2161 taactagctc taatatgcaa atatgtggat ttaaaagcac agttccgcat ttcaccgaag
     2221 aagaaaagta tcaaaagctt ctcagtgaaa ataaaattag agatgaacag cctaaacatc
     2281 agccagatat atgtggtaag aactttaaca caaatttgtt tcagttgggc cataaatgtg
     2341 cagctgtgtt ggatttggtt tgtagtactg aaaaaattaa tatagggcct gaagtggtac
     2401 aaagagagtg tgtgccaaca gaatatcatg aaatacaaaa ccagtgtttg ggattatttt
     2461 cctcgaacgc agtagataag tcaaggtctg aagcagcagt taggaaggtc tcagacctta
     2521 aaatatcaac tgatacagaa tttctcagta taattacctc cagccaggtt gcttttttag
     2581 ctcaaaagaa agataaaagg cggagtcctg taaataaagg gaatgtaaac atggagactg
     2641 aaccaaaggc aagttacggg gagataagaa tacctgaaga gaattcgatt cagcttgatg
     2701 gttttacaga agcatatgaa agtggacaaa accaagcata ttcccttgaa ctttttagtc
     2761 ctgtttgtcc taaaacagaa aatagccgca ttcacataaa ctctgataaa ggtcttgaag
     2821 aacatacagg atctcaagaa cttttcagtt ctgaagatga actgccacca aatgagatac
     2881 gtattgagtt gtgtagctca ggaatactgt gttcccaact aaataccttc cacaaaagtg
     2941 ctattaaaag aagctgtacc tctgaagata aagtgggcca gtctgaagct ctatctagag
     3001 tccttcaagt agctaagaaa atgaagttga tttctaatgg aggagattct gctgtagaaa
     3061 tggatcggag aaatgtgtct gaatttaaga gtattaaaaa aacatcatta ataaaaaact
     3121 gtgattctaa aagccagaag tataattgtt tagtcatggt gctatctcca tgccatgtga
     3181 aggaaataaa cataaaattc ggaccaaatt ctggctctaa agtgccttta gcaacagtta
     3241 cagtaattga tcaatcagaa actaagaaga aggtttttct gtggaggact gcagcatttt
     3301 gggcatttac agtgtttctt ggagatataa ttttactcac agatgttgtt attcatgagg
     3361 accaatggat tggcgagaca gtactacaat caacatttag cagtcagtta ttaaatcttg
     3421 ggagttattc atctattcag cctgaagaat attccagtgt agttagtgaa gttgtacttc
     3481 aagacttact ggcatatgtg tcctcaaaac attcctacct cagagatctt cctccgaggc
     3541 agcctcagag ggtgaacagt atagactttg tagaattgga gcaccttcaa cctgatgtat
     3601 tagtccacgc agtactaaga gttgttgatt tcactatact gacagaggca gtatacagtt
     3661 atagaggaca gaagcagaaa aaagttatgt taacagtgga acaggcccaa gatcaacatt
     3721 atgcgcttgt attatggggt cctggagcag cctggtaccc tcaacttcaa aggaaaaaag
     3781 gttatatttg ggaatttaaa tatctttttg ttcagtgcaa ttacacacta gaaaacctag
     3841 aattgcatac aacgccttgg tcatcctgtg agtgcttgtt tgatgatgat ataagggcaa
     3901 ttacatttaa agcaaaattt caaaaaagtg caccctcctt tgtgaagata tcagacttag
     3961 caacccacct agaggataag tgttcaggag tggttctgat taaagcccag atttcagagc
     4021 tggcatttcc tattacagca tctcagaaga tagcgctaaa tgctcacagt tctctgaaga
     4081 gtattttttc ttctcttccc aacatcgtat atactggttg tgcaaaatgt ggattggaac
     4141 tagaaacaga tgagaacagg atctacaaac aatgttttag ctgcttgcca tttactatga
     4201 agaaaatata ttataggcca gcgttaatga ctgccattga tggaagacat gatgtttgta
     4261 tccgtgtaga atcaaagctg atagagaaga ttcttctcaa catttctgca gactgcctca
     4321 acagagtgat agttccttcc tcagagatca cctatgggat ggtcgtggca gacctgttcc
     4381 actccttgtt ggcagtcagc gcagaacctt gtgtattaaa gattcagagc ctttttgtgt
     4441 tagatgaaaa cagctatcca ttacaacaag atttctccct cctggatttt tatcctgaca
     4501 ttgtaaagca tggagccaat gcccgtctct aggacccagc tttcttgtac aaagtggttt
     4561 agtaatgaac cggtccacca ccaccaccac cactaaggat ccggggttgg ggttgcgcct
     4621 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4681 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4741 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4801 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4861 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4921 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4981 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     5041 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     5101 cctctctccc cagcaattca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     5161 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5221 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5281 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     5341 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     5401 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     5461 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     5521 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5581 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5641 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5701 gacccgcaag cccggtgccg aaggtagagg ttctctcctc acttgtggtg atgttgaaga
     5761 aaaccctggt ccaatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt
     5821 actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     5881 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
     5941 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     6001 agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg
     6061 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     6121 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     6181 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
     6241 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     6301 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     6361 cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga
     6421 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     6481 cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atcgatccaa
     6541 gatatcgtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6601 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6661 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6721 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6781 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6841 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6901 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6961 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7021 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7081 gggccgcctc cccgcctgtt tcgcctcggc gtccggacta gaggtacctt taagaccaat
     7141 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7201 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     7261 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7321 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7381 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7441 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     7501 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7561 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7621 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     7681 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7741 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctttca
     7801 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7861 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7921 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7981 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     8041 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     8101 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8161 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8221 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8281 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8341 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8401 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8461 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8521 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8581 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8641 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8701 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8761 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8821 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8881 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8941 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9001 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     9061 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9121 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9181 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9241 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9301 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9361 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9421 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9481 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9541 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9601 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9661 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9721 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     9781 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     9841 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     9901 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     9961 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
    10021 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
    10081 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
    10141 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
    10201 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    10261 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    10321 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
    10381 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
    10441 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
    10501 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
    10561 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
    10621 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
    10681 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
    10741 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
    10801 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
    10861 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
    10921 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
    10981 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
    11041 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
    11101 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
    11161 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
    11221 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
    11281 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
    11341 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
    11401 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
    11461 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
    11521 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
    11581 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
    11641 acctcccaac cccgagggg
//
