LOCUS       sequence-216024-       10663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Sharon E, Chen SA, Khosla NM, Smith JD, Pritchard JK, Fraser HB
  TITLE     Functional Genetic Variants Revealed by Massively Parallel Precise 
            Genome Editing.
  JOURNAL   Cell. 2018 Sep 18. pii: S0092-8674(18)31118-8. doi: 
            10.1016/j.cell.2018.08.057.
  PUBMED    30245013
REFERENCE   2  (bases 1 to 10663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2018 
            Sep 18. pii: S0092-8674(18)31118-8. doi: 
            10.1016/j.cell.2018.08.057."
COMMENT     Sequence Label: pZS157 CRISPEY RT/Cas9
FEATURES             Location/Qualifiers
     source          1..10663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(620..806)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(953..972)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1072..1094
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1132..1150)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1218..1322
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1323..1391,1392..2183)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1541..1560)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2354..2942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2843..2862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3096..3113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(3266..3271,3272..3289,3290..3296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3304..3320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3309..3331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3328..3344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3328..3344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3363..3383
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3365..3383
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(3422..3611)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     3623..3641
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(3673..3693)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3706..7809)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3706..7809)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3706..7809)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(7842..8283)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(7848..7871)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    8166..8283
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             8505..8525
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9718..9737)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9719..9737)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9747..9764)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9747..9763)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9756..9778)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9908..10363
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9995..10014)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10205..10226
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(10623..10663,1..619))
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 cattgggcga ggtggcttct cttatggcaa ccgcaagagc cttgaacgca ctctcactac
       61 ggtgatgatc attcttgcct cgcagacaat caacgtggag ggtaattctg ctagcctctg
      121 caaagctttc aagaaaatgc gggatcatct cgcaagagag atctcctact ttctcccttt
      181 gcaaaccaag ttcgacaact gcgtacggcc tgttcgaaag atctaccacc gctctggaaa
      241 gtgcctcatc caaaggcgca aatcctgatc caaacctttt tactccacgc gccagtaggg
      301 cctctttaaa agcttgaccg agagcaatcc cgcagtcttc agtggtgtga tggtcgtcta
      361 tgtgtaagtc accaatgcac tcaacgatta gcgaccagcc ggaatgcttg gccagagcat
      421 gtatcatatg gtccagaaac cctatacctg tgtggacgtt aatcacttgc gattgtgtgg
      481 cctgttctgc tactgcttct gcctcttttt ctgggaagat cgagtgctct atcgctaggg
      541 gaccaccctt taaagagatc gcaatctgaa tcttggtttc atttgtaata cgctttacta
      601 gggctttctg ctctgtcatc tttgccttcg tttatcttgc ctgctcattt tttagtatat
      661 tcttcgaaga aatcacatta ctttatataa tgtataattc attatgtgat aatgccaatc
      721 gctaagaaaa aaaaagagtc atccgctagg ggaaaaaaaa aaatgaaaat cattaccgag
      781 gcataaaaaa atatagagtg tactagagga ggccaagagt aatagaaaaa gaaaattgcg
      841 ggaaaggact gtgttatgac ttccctgact aatgccgtgt tcaaacgata cctggcagtg
      901 actcctagcg ctcaccaagc tcttaaaacg ggaatttatg gtgcactctc agtacaatct
      961 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     1021 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     1081 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     1141 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1441 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1501 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1561 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1621 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1681 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1741 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1801 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1861 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1921 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1981 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2041 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2101 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2161 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2221 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2461 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3001 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3061 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3121 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     3241 acctcactca ttaggcaccc caggctttac actttatgct tccggctcct atgttgtgtg
     3301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     3361 gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt gaaattaaag
     3421 ccttcgagcg tcccaaaacc ttctcaagca aggttttcag tataatgtta catgcgtaca
     3481 cgcgtctgta cagaaaaaaa agaaaaattt gaaatataaa taacgttctt aatactaaca
     3541 taactataaa aaaataaata gggacctaga cttcaggttg tctaactcct tccttttcgg
     3601 ttagagcgga tgtgggggga gggcgtgaat gtaagcgtga cataactaat tacatgactc
     3661 gagaagagat cacaccttcc tcttcttctt ggggtcagcc ctgctgtctc caccgagctg
     3721 agagaggtcg attcttgttt catagagccc cgtaattgac tgatgaatca gtgtggcgtc
     3781 caggacctcc tttgtagagg tgtaccgctt tctgtctatg gtggtgtcga agtacttgaa
     3841 ggctgcaggc gcgcccaagt tggtcagagt aaacaagtgg ataatgtttt ctgcctgctc
     3901 cctgatgggc ttatccctgt gcttattgta agcagaaagc accttatcga ggttagcgtc
     3961 ggcgaggatc actcttttgg agaattcgct tatttgctcg atgatctcat caaggtagtg
     4021 tttgtgttgt tccacgaaca gctgcttctg ctcattatct tcgggagacc ctttgagctt
     4081 ttcatagtgg ctggccagat acaagaaatt aacgtattta gagggcagtg ccagctcgtt
     4141 acctttctgc agctcgcccg cactagcgag cattcgtttc cggccgtttt caagctcaaa
     4201 gagagagtac ttgggaagct taatgatgag gtcttttttg acctctttat atcctttcgc
     4261 ctcgagaaag tcgatggggt ttttttcgaa gcttgatcgc tccatgattg tgatgcccag
     4321 cagttccttg acgcttttga gttttttaga cttccctttc tccactttgg ccacaaccag
     4381 tacactgtaa gcgactgtag gagaatcgaa tccgccgtat ttcttggggt cccaatcttt
     4441 tttgcgtgcg atcagcttgt cgctgttcct tttcgggagg atactttcct tggagaagcc
     4501 tccggtctgt acttcggtct ttttaacgat gttcacctgc ggcatggaca ggaccttccg
     4561 gactgtcgcg aaatccctac ccttgtccca cacgatttct cctgtttctc cgtttgtttc
     4621 gataagtggt cgcttccgaa tctctccatt ggccagtgta atctcggtct tgaaaaaatt
     4681 cataatattg ctgtaaaaga agtacttagc ggtggccttg cctatttcct gctcagactt
     4741 tgcgatcatt ttcctaacat cgtacacttt atagtctccg taaacaaatt cagattcaag
     4801 cttgggatat tttttgataa gtgcagtgcc taccactgca ttcaggtagg catcatgcgc
     4861 atggtggtaa ttgttgatct ctctcacctt ataaaactga aagtcctttc tgaaatctga
     4921 gaccagctta gacttcagag taataacttt cacctctcga atcagtttgt cattttcatc
     4981 gtacttggtg ttcatgcgtg aatcgagaat ttgggccacg tgcttggtga tctggcgtgt
     5041 ctcaacaagc tgccttttga tgaagccggc tttatccaac tcagacaggc cacctcgttc
     5101 agccttagtc agattatcga acttccgttg tgtgatcagt ttggcgttca gcagctgccg
     5161 ccaataattt ttcattttct tgacaacttc ttctgagggg acgttatcac tcttccctct
     5221 atttttatcg gatcttgtca acactttatt atcaatagaa tcatctttga gaaaagactg
     5281 gggcacgata tgatccacgt cgtagtcgga gagccgattg atgtccagtt cctgatccac
     5341 gtacatgtcc ctgccgttct gcaggtagta caggtagagc ttctcattct gaagctgggt
     5401 gttttcaact gggtgttcct taaggatttg ggaccccagt tcttttatac cctcttcaat
     5461 cctcttcatc ctttccctac tgttcttctg tcccttctgg gtagtttggt tctctcgggc
     5521 catctcgata acgatattct cgggcttatg ccttcccatt actttgacga gttcatccac
     5581 gaccttaacg gtctgcagta ttcccttttt gatagctggg ctacctgcaa gattagcgat
     5641 gtgctcgtga agactgtccc cctggccaga aacttgtgct ttctggatgt cctccttaaa
     5701 ggtgagagag tcatcatgga tcaactgcat gaagttccgg ttggcaaatc catcggactt
     5761 aagaaaatcc aggattgtct ttccactctg cttgtctcgg atcccattga tcagttttct
     5821 tgacagccgc ccccatcctg tatatcggcg cctcttgagc tgtttcatga ctttgtcgtc
     5881 gaagagatga gcgtaagttt tcaagcgttc ttcaatcatc tccctatctt caaacaacgt
     5941 aagggtgagg acaatgtcct caagaatgtc ctcgttctcc tcattgtcca ggaagtcctt
     6001 gtctttaatg attttcagga gatcgtgata cgttcccagg gatgcgttga agcgatcctc
     6061 cactccgctg atttcaacag agtcgaaaca ttcaatcttt ttgaaatagt cttctttgag
     6121 ctgtttcacg gtaactttcc ggttcgtctt gaagaggagg tccacgatag ctttcttctg
     6181 ctctccagac aggaatgctg gctttctcat cccttctgtg acgtatttga ccttggtgag
     6241 ctcgttataa actgtgaagt actcgtacag cagagagtgt ttaggaagca ccttttcgtt
     6301 aggcagattt ttatcaaagt tagtcatcct ttcgatgaag gactgggcag aggccccctt
     6361 atccacgact tcctcgaagt tccagggagt gatggtctct tctgatttgc gagtcatcca
     6421 cgcgaatctg gaatttcccc gggcgagggg gcctacatag tagggtatcc gaaatgtgag
     6481 gattttctca atcttttccc tgttatcttt caaaaagggg tagaaatcct cttgccgcct
     6541 gaggatagcg tgcagttcgc ccaggtgaat ctggtggggg atgcttccat tgtcgaaagt
     6601 gcgctgtttg cgcaacagat cttctctgtt aagctttacc agcagctcct cggtgccgtc
     6661 cattttttcc aagatgggct taataaattt gtaaaattcc tcctggcttg ctccgccgtc
     6721 aatgtatccg gcgtagccat ttttagactg atcgaagaaa atttccttgt acttctcagg
     6781 cagttgctgt ctgacaaggg ccttcagcaa agtcaagtct tggtggtgct catcatagcg
     6841 cttgatcata ctagcgctca gcggagcttt ggtgatctcc gtgttcactc gcagaatatc
     6901 actcagcaga atggcgtctg acaggttctt tgccgccaaa aaaaggtctg cgtactggtc
     6961 gccgatctgg gccagcagat tgtcgagatc atcatcgtag gtgtctttgc tcagttgaag
     7021 cttggcatct tcggccaggt cgaagttaga tttaaagttg ggggtcagcc cgagtgacag
     7081 ggcgataaga ttaccaaaca ggccgttctt cttctcccca gggagctgtg cgatgaggtt
     7141 ttcgagccgc cgggatttgg acagcctagc gctcaggatt gctttggcgt caactccgga
     7201 tgcgttgatc gggttctctt cgaaaagctg attgtaagtc tgaaccagtt ggataaagag
     7261 tttgtcgaca tcgctgttgt ctgggttcag gtccccctcg atgaggaagt gtccccgaaa
     7321 tttgatcata tgcgccagcg cgagatagat caaccgcaag tcagccttat cagtactgtc
     7381 tacaagcttc ttcctcagat gatatatggt tgggtacttt tcatggtacg ccacctcgtc
     7441 cacgatattg ccaaagattg ggtggcgctc gtgcttttta tcctcctcca ccaaaaagga
     7501 ctcctccagc ctatggaaga aagagtcatc caccttagcc atctcattac taaagatctc
     7561 ctgcaggtag cagatccgat tctttctgcg ggtatatctg cgccgtgctg ttcttttgag
     7621 ccgcgtggct tcggccgtct ccccggagtc gaacaggagg gcgccaatga ggttcttctt
     7681 tatgctgtgg cgatcggtat tgcccagaac tttgaatttt ttgctcggca ccttgtactc
     7741 gtccgtaatg acggcccagc cgacgctgtt tgtgccgata tcgagcccaa tggagtactt
     7801 cttgtccatt tttttcccgg gggatccact agttgttttt tctccttgac gttaaagtat
     7861 agaggtatat taacaatttt ttgttgatac ttttatgaca tttgaataag aagtaataca
     7921 aactgaaaat gttgaaagta ttagttaaag tggttatgca gcttttccat ttatatatct
     7981 gttaatagat caaaaatcat cgcttcgctg attaattacc ccagaaataa ggctaaaaaa
     8041 ctaatcgcat tatcatccta tggttgttaa tttgattcgt taatttgaag gtttgtgggg
     8101 ccaggttact gccaattttt cctcttcata accataaaag ctagtattgt agaatcttta
     8161 ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac
     8221 gaggacgcac ggaggagagt cttccgtcgg agggctgtcg cccgctcggc ggcttctaat
     8281 ccgtacttca atatagcaat gagcagttaa gcgtattact gaaagttcca aagagaaggt
     8341 ttttttaggc taagataatg gggctcttta catttccaca acatataagt aagattagat
     8401 atggatatgt atatggtggt aatgccatgt aatatgatta ttaaacttct ttgcgtccat
     8461 ccaaaaaaaa agtaagaatt tttgaaaatt caatataaat gccaccaaag aagaaaagaa
     8521 aggtcaagtc cgctgaatac ttgaacacct tcagattgag aaatttgggt ttgccagtca
     8581 tgaacaactt gcacgatatg tctaaggcta ccagaatttc tgttgaaacc ttgagattat
     8641 tgatctacac cgccgatttc agatacagaa tctacactgt tgaaaagaag ggtccagaaa
     8701 agagaatgag aactatctac caaccatcca gagaattgaa ggctttacaa ggttgggttt
     8761 tgagaaacat cttggacaag ttatcctcct ccccattttc tatcggtttt gaaaagcacc
     8821 aatccatctt gaacaatgcc actccacata ttggtgccaa cttcattttg aacatcgact
     8881 tggaagattt cttcccatct ttgactgcta acaaggtttt cggtgttttc cattctttgg
     8941 gttacaacag attgatctcc tctgttttga ccaagatttg ctgctacaag aacttgttgc
     9001 cacaaggtgc tccatcttct ccaaaattgg ctaacttgat ttgctccaag ttggactaca
     9061 gaattcaagg ttatgctggt tctagaggtt tgatatacac tagatacgct gatgacttga
     9121 ccttgtctgc tcaatctatg aagaaagttg ttaaggccag agacttcttg ttctccatta
     9181 ttccatcaga aggtttggtc atcaactcca aaaagacctg tatttctggt ccaagatccc
     9241 aaagaaaagt taccggtttg gttatctccc aagaaaaggt tggtattggt agagaaaagt
     9301 acaaagaaat cagagccaag atccaccata ttttctgcgg taaatcctcc gaaatcgaac
     9361 atgttagagg ttggttgtct ttcatcttgt ccgttgattc taagtcccac agaagattga
     9421 ttacctacat ctctaagttg gaaaagaagt acggtaagaa cccattgaac aaggctaaga
     9481 cttgatttgc cagcttacta tccttcttga aaatatgcac tctatatctt ttagttctta
     9541 attgcaacac atagatttgc tgtataacga attttatgct attttttaaa tttggagttc
     9601 agtgataaaa gtgtcacagc gaatttcctc acatgtaggg accgaattgt ttacaagttc
     9661 tctgtaccac catggagaca tcaaaaattg aaaatctatg gaaagatatg caattcgccc
     9721 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     9781 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     9841 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     9901 tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     9961 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    10021 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    10081 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    10141 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    10201 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    10261 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    10321 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcctgatg cggtattttc
    10381 tccttacgca tctgtgcggt atttcacacc gcatagatcc gtcgagttca agagaaaaaa
    10441 aaagaaaaag caaaaagaaa aaaggaaagc gcgcctcgtt cagaatgaca cgtatagaat
    10501 gatgcattac cttgtcatct tcagtatcat actgttcgta tacatactta ctgacattca
    10561 taggtataca tatatacaca tgtatatata tcgtatgctg cagctttaaa taatcggtgt
    10621 cactacataa gaacaccttt ggtggaggga acatcgttgg tac
//
