LOCUS       sequence-216034-       12699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human ATG2A with GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12699)
  AUTHORS   Tamura N, Nishimura T, Sakamaki Y, Koyama-Honda I, Yamamoto H, 
            Mizushima N
  TITLE     Differential requirement for ATG2A domains for localization to 
            autophagic membranes and lipid droplets.
  JOURNAL   FEBS Lett. 2017 Dec;591(23):3819-3830. doi: 10.1002/1873-3468.12901.
            Epub 2017 Nov 20.
  PUBMED    29113029
REFERENCE   2  (bases 1 to 12699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1873-3468.12901"; journalName: "FEBS Lett"; date: 
            "2017-12"; volume: "591"; issue: "23"; pages: "3819-3830"
COMMENT     Sequence Label: pMRXIP-GFP-hATG2A
FEATURES             Location/Qualifiers
     source          1..12699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    667..1024
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1089..1505
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1435..1457
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1473..1489
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1515..1889
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1827..1843
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(1901..1903,1904..1906,1907..2617)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1901..1903,1904..1906,1907..2617)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1946..1967)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2207..2226)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2554..2575
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8550..9116
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8566..9118
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8710..8727)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8937..8956
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9130..9729
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9130..9149)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9626..9646
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9953..10422
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(10552..10569)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10723..11311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10803..10822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11475..12266,12267..12335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12098..12117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12336..12440)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12508..12526
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(12628..12699,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatgct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
      361 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
      421 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact
      481 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
      541 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
      601 catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg
      661 ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga
      721 ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg
      781 tggaactgac gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg
      841 gggccgtttt tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg
      901 cacccccctt agaggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc
      961 cgcctccgtc tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct
     1021 gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaata
     1081 tgggcccggg ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg
     1141 tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct
     1201 gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag
     1261 acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc
     1321 aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc
     1381 cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg
     1441 aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag
     1501 gcgcccccat atggccatat gagatcttat atggggcacc cccgcccctt gtaaacttcc
     1561 ctgaccctga catgacaaga gttactaaca gcccctctct ccaagctcac ttacaggctc
     1621 tctacttagt ccagcacgaa gtctggagac ctctggcggc agcctaccaa gaacaactgg
     1681 accgaccggt ggtacctcac ccttaccgag tcggcgacac agtgtgggtc cgccgacacc
     1741 agactaagaa cctagaacct cgctggaaag gaccttacac agtcctgctg accaccccca
     1801 ccgccctcaa agtagacggc atcgcagctt ggatacacgc cgcccacgtg aaggctgccg
     1861 accccggggg tggaccatcc tctagactgc cggatctgcc atggtgagca agggcgagga
     1921 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1981 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2041 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2101 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2161 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2221 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2281 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2341 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2401 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2461 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2521 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2581 cgccgggatc actctcggca tggacgagct gtacaagggt ggcggtggct cgggcggtgg
     2641 tgggtcgggt ggcggcggat ctatcgatga attggaattc atgtcacgat ggctgtggcc
     2701 atggtcaaac tgtgtgaaag agcgggtctg ccgctacttg ctgcaccact acttaggtca
     2761 cttcttccaa gagcacctca gcctggacca gctcagcctc gatctgtaca agggcagcgt
     2821 tgccctgcga gacatccacc tggaaatctg gtctgtgaac gaggtgctgg agtcaatgga
     2881 gtcaccgctg gagctggtgg aaggcttcgt gggctccatc gaggtggccg tgccctgggc
     2941 tgctctgctc accgaccact gcacagtgcg cgtgtccggc ctccagctca ccttgcagcc
     3001 ccgccggggt ccagcgccag gggctgccga ctcacagagc tgggcctcat gcatgaccac
     3061 aagcctgcag ctggcccagg agtgtctgcg ggatgggcta ccggagccct ctgagccacc
     3121 acagcccctg gaggggctgg agatgtttgc ccagaccatt gagactgtgc ttcggaggat
     3181 caaagtgacc ttcctggaca ctgtcgtgag ggtggagcac tctccgggtg atggggaacg
     3241 tggtgtggcc gtcgaggtcc gtgtgcagag actggagtac tgtgatgagg cagtgcggga
     3301 cccaagccag gcgccgccgg tggacgtgca tcagccgcct gccttcctgc acaagctgct
     3361 gcagctggca ggggtccgcc tgcactacga ggagctcccg gcacaggaag agcctccaga
     3421 gccccccttg cagatcggca gctgctcagg gtacatggag ctgatggtga agttgaagca
     3481 aaatgaggcc ttccctggcc ccaagttgga ggtggcggga cagctgggct ccctgcacct
     3541 gctcctgacc ccgaggcagc tccagcaact tcaggaactg ctcagcgccg tgagccttac
     3601 agaccacgag ggcctggctg acaagctgaa caagagccgc ccgctaggtg ccgaagacct
     3661 gtggctgatt gagcaggacc tgaaccagca gctgcaggca ggggcagtgg ctgagcccct
     3721 cagcccagac ccccttacca acccccttct caacctggat aacactgacc tcttcttctc
     3781 catggctggc ctcacaagca gtgtggcctc agccctctct gagctctccc tctccgatgt
     3841 agacctggcc tcctctgtgc gcagcgacat ggcctcccgc cggctctctg cccaggccca
     3901 cccagctggc aagatggccc ccaaccccct cctggacacc atgcgccctg actcgctgct
     3961 gaagatgacc ttggggggtg tgaccctgac cttgcttcag acgtctgccc catcttccgg
     4021 accacctgac ctcgccacgc actttttcac cgagtttgat gccaccaagg atgggccctt
     4081 cggttcccga gacttccatc accttcgacc acgcttccag agggcctgtc cctgtagcca
     4141 tgttcggcta acgggcacag ccgtgcagct gtcctgggag ctgcggacgg gcagtcgggg
     4201 ccggcggaca accagcatgg aagtgcactt cgggcagctg gaggtgctgg agtgtctgtg
     4261 gccccggggc acctctgagc ctgagtacac ggagatcctg acctttcctg gtacgctggg
     4321 ctcccaggcc tcagctcggc cctgcgccca tctgcgccac acacagatcc tgcgccgtgt
     4381 gcctaagagc cgaccccggc gctcagttgc ctgccattgc cactcagaac tggccctgga
     4441 cctggccaac ttccaggcgg acgtggagct gggggccctg gaccggctgg ccgccctact
     4501 gcgcctggcc accgtacctg ctgagcctcc agccggcctg ctgacagagc ccctgccggc
     4561 gatggagcag cagacggtat ttcggctctc tgcaccccgg gccacgctgc ggctgcgctt
     4621 ccccattgcc gacctgcggc ctgagccgga cccctgggcg ggccaggccg tgcgggctga
     4681 gcagcttcgg ctggagctga gtgagcccca gttccggtca gagcttagca gtgggcctgg
     4741 tcccccagtc cccacccacc tggaactcac ctgctccgac ctacatggta tctatgaaga
     4801 tggagggaag ccacctgtcc cttgcctgcg tgtctccaaa gccctggacc ccaagagcac
     4861 tgggcgcaag tacttcctgc cccaggtagt ggtgactgtg aacccccagt ccagcagcac
     4921 acagtgggag gtggccccgg agaagggaga ggaactggag ctgtcagtgg agagtccctg
     4981 tgagctgcgg gaacctgagc cctcgccctt ctcctctaag aggaccatgt atgagacaga
     5041 ggagatggtg atccctggag accctgagga gatgaggacg ttccagagcc ggaccctggc
     5101 actgtcccgc tgcagcctgg aagtgatcct gcccagtgtc cacatctttc tgcccagcaa
     5161 ggaggtctac gagagcatct acaacaggat caacaacgac ctgctcatgt gggagcctgc
     5221 agatctgctt cccacccccg accccgccgc ccagccctcg ggcttccccg gcccctcagg
     5281 cttctggcac gacagcttta agatgtgcaa gtcagccttc aagctggcca actgctttga
     5341 tctcacccca gactcggact cggatgacga ggatgcccac ttcttctcag tgggggcatc
     5401 aggtggccca caggccgctg cccctgaggc cccaagtctt cacttgcaga gcaccttctc
     5461 tacactggtg acagtgctga aggggcggat cacagccctc tgtgagacca aggatgaggg
     5521 tgggaagcgg ctggaggctg tgcacgggga gctggtgctg gacatggagc acggtaccct
     5581 cttcagcgtc tcccagtact gtggccagcc aggacttggc tacttctgtc tggaagctga
     5641 aaaggcaaca ctctaccacc gagcggccgt ggatgactac ccgctgccca gtcacctgga
     5701 ccttcccagt ttcgctcccc cggctcagct ggccccaacc atctacccat cggaggaagg
     5761 ggtgaccgag cggggagcct cgggccgcaa gggccagggc cggggacccc acatgttgtc
     5821 cactgctgtg cgcatccacc tggaccccca caagaatgtg aaggagttcc tggtgacact
     5881 gcggttgcac aaagccacct tgcgccacta catggccctg cccgagcaga gctggcattc
     5941 ccagttgttg gagttcctag acgtgctgga tgaccctgtg ctgggctacc tgcccccgac
     6001 ggtcatcacc atcctgcaca cacacctgtt ctcctgctct gtggactata ggccactcta
     6061 cctcccagtg cgtgtcctca tcaccgcgga gaccttcact ctctccagca acatcatcat
     6121 ggacacctcc accttcctgc tcaggttcat cctcgatgac tccgccttgt acctgtccga
     6181 caagtgtgag gtggagaccc tggacctgcg gcgagattat gtctgtgttt tggatgttga
     6241 cctcttggaa cttgtgatta aaacctggaa agggagcacc gagggcaaac tgagccagcc
     6301 actattcgag ctgcgctgct ccaacaatgt ggtacacgtg cacagctgtg ccgactcctg
     6361 tgccctgctg gtcaacctgc tccagtacgt aatgagcaca ggcgatctgc accccccacc
     6421 ccggcccccc agccccacgg agatcgccgg ccagaagctc tcggagagtc ctgcctctct
     6481 gccctcgtgc cccccagtgg agacggccct catcaaccag cgtgacctgg ccgacgccct
     6541 cctggacacc gagcgcagcc tacgggagct ggcccagcct tcaggtggcc acctccctca
     6601 ggcgtcgccc atctccgtct acctattccc aggtgaacgg agtggggccc cacccccttc
     6661 accacctgtc gggggccctg ctggcagctt agggtcatgc tcagaggaga aggaagatga
     6721 aagggaagag gagggcgatg gagacaccct ggacagtgat gagttctgca tccttgatgc
     6781 tcccggcctg ggcatcccgc cccgagatgg ggagcctgtg gtgacacagc tgcatcccgg
     6841 ccccatcgtt gtgagggacg gttacttctc acggccgatc ggcagcacgg acttgctgcg
     6901 ggcacctgcc catttcccag tgcccagcac tcgggtggtg ctacgtgagg tctccctcgt
     6961 ctggcacctc tatgggggcc gagactttgg cccccacccc ggccacaggg caagaactgg
     7021 cctctcaggt cccaggagct ccccttcccg ctgctctggc cccaaccggc cccagaactc
     7081 atggcgcacg caggggggca gcgggcggca gcaccatgtc ctcatggaga tccagctgag
     7141 caaggtaagc ttccagcacg aggtgtaccc agcggagcca gccacaggcc ctgcggcccc
     7201 cagccaggag ctggaggagc gaccgctgtc ccgtcaggtg ttcatcgtgc aggagctgga
     7261 ggtccgagac cggctcgcct cctcccagat caacaagttc ctgtacctac acacgagtga
     7321 gcggatgccg cgacgtgccc actctaacat gctcaccatc aaagcgctgc atgtggcccc
     7381 cactaccaac ctgggtgggc ctgagtgctg tctccgcgtc tcgctgatgc ccctgcggct
     7441 caatgtggac caggatgccc tcttcttcct caaggacttc ttcactagtc tggtggccgg
     7501 catcaacccc gtggtcccag gggagacctc cgctgaggct cgccccgaga ctcgagccca
     7561 gcccagcagc cccctggaag ggcaggccga aggcgtagag accactggtt cgcaggaggc
     7621 cccaggaggt ggacacagcc cctcccctcc tgaccagcag cccatctact tcagagagtt
     7681 ccgcttcacg tctgaggtcc ccatctggct ggattaccat ggcaagcacg tcacgatgga
     7741 ccaggtgggc acttttgctg gcctcctcat cggcctggcc caactcaact gctccgagct
     7801 gaagctaaag cggctctgtt gcaggcacgg gctcctgggt gtggacaagg tgctgggcta
     7861 tgccctcaac gagtggctgc aggacatccg caagaaccag ctgcccggcc tgctgggagg
     7921 cgtgggcccc atgcactcgg ttgtccagct cttccaaggg ttccgggacc tgctgtggct
     7981 gcccattgag cagtacagga aggatggccg cctcatgcgg gggctgcagc gaggggctgc
     8041 ctcctttggc tcatccacag cctctgccgc cctggaactc agcaaccggt tggtacaggc
     8101 tatccaggcc acagctgaga ccgtgtatga catcctgtcc ccggcagccc ccgtctcccg
     8161 ctccctgcag gataagcgct ctgcgcggag gctgcgcagg ggccagcagc ctgccgacct
     8221 gcgggagggt gtggccaagg cctacgacac agtgcgagag ggcatcttgg atacagctca
     8281 gaccatctgt gacgtggcat cgcggggcca tgagcagaag gggctgacgg gcgccgtggg
     8341 gggcgtgatc cgccagctgc ccccgactgt ggtgaagccg ctcatcctgg ccacggaggc
     8401 cacgtccagc ctgctcgggg gcatgcgcaa ccagattgtc cccgacgccc acaaggacca
     8461 cgccctcaag tggcgctcgg acagtgccca agactgagaa ttcacgcgtt cgcgagttaa
     8521 cggatccctc gagcggccgc tacgtaaatt ccgccccccc ccctaacgtt actggccgaa
     8581 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     8641 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     8701 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     8761 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     8821 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     8881 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     8941 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     9001 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     9061 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     9121 gccacaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     9181 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     9241 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     9301 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     9361 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     9421 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     9481 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     9541 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     9601 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     9661 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     9721 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     9781 ggctcgacga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     9841 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     9901 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     9961 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
    10021 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
    10081 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
    10141 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
    10201 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
    10261 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
    10321 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
    10381 tctcctctga gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa
    10441 attaatttgg ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat
    10501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10561 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10621 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10681 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10741 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10801 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10861 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10921 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10981 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11041 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11101 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    11161 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11221 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11281 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11341 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    11401 gatcttcacc tagatccttt tgcggccggc cgcaaatcaa tctaaagtat atatgagtaa
    11461 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    11521 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    11581 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11641 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11701 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11761 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11821 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11881 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11941 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    12001 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    12061 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
    12121 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    12181 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    12241 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    12301 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    12361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    12421 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    12481 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac
    12541 tcgaggagct tggcccattg catacgttgt atccatatca taatatgtac atttatattg
    12601 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
    12661 caattacggg gtcattagtt catagcccat atatggagt
//
