LOCUS       sequence-216046-       15572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intermediate vector containing HTT gene, except for polyQ region. 
            Used to clone various polyQ lengths into HTT sequence with C-term 
            FLAG tag. Baculovirus transfer vector for insect and mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15572)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C entry vector
FEATURES             Location/Qualifiers
     source          1..15572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      13625..13634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14320..14343
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14365..14394
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14395..14418
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14508..14528)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14926..14937)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15162..15296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15199..15218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15253..15272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15325..15490
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa
     5101 gcgctgacct tagtcgctaa cctgcaggag ccgctgcacc gaccaaagaa agaactttca
     5161 gctaccaaga aagaccgtgt gaatcattgt ctgacaatat gtgaaaacat agtggcacag
     5221 tctgtcagaa attctccaga atttcagaaa cttctgggca tcgctatgga actttttctg
     5281 ctgtgcagtg atgacgcaga gtcagatgtc aggatggtgg ctgacgaatg cctcaacaaa
     5341 gttatcaaag ctttgatgga ttctaatctt ccaaggttac agctcgagct ctataaggaa
     5401 attaaaaaga atggtgcccc tcggagtttg cgtgctgccc tgtggaggtt tgctgagctg
     5461 gctcacctgg ttcggcctca gaaatgcagg ccttacctgg tgaaccttct gccgtgcctg
     5521 actcgaacaa gcaagagacc cgaagaatca gtccaggaga ccttggctgc agctgttccc
     5581 aaaattatgg cttcttttgg caattttgca aatgacaatg aaattaaggt tttgttaaag
     5641 gccttcatag cgaacctgaa gtcaagctcc cccaccattc ggcggacagc ggctggatca
     5701 gcagtgagca tctgccagca ctcaagaagg acacaatatt tctatagttg gctactaaat
     5761 gtgctcttag gcttactcgt tcctgtcgag gatgaacact ccactctgct gattcttggc
     5821 gtgctgctca ccctgaggta tttggtgccc ttgctgcagc agcaggtcaa ggacacaagc
     5881 ctgaaaggca gcttcggagt gacaaggaaa gaaatggaag tctctccttc tgcagagcag
     5941 cttgtccagg tttatgaact gacgttacat catacacagc accaagacca caatgttgtg
     6001 accggagccc tggagctgtt gcagcagctc ttcagaacgc ctccacccga gcttctgcaa
     6061 accctgaccg cagtcggggg cattgggcag ctcaccgctg ctaaggagga gtctggtggc
     6121 cgaagccgta gtgggagtat tgtggaactt atagctggag ggggttcctc atgcagccct
     6181 gtcctttcaa gaaaacaaaa aggcaaagtg ctcttaggag aagaagaagc cttggaggat
     6241 gactctgaat cgagatcgga tgtcagcagc tctgccttaa cagcctcagt gaaggatgag
     6301 atcagtggag agctggctgc ttcttcaggg gtttccactc cagggtcagc aggtcatgac
     6361 atcatcacag aacagccacg gtcacagcac acactgcagg cggactcagt ggatctggcc
     6421 agctgtgact tgacaagctc tgccactgat ggggatgagg aggatatctt gagccacagc
     6481 tccagccagg tcagcgccgt cccatctgac cctgccatgg acctgaatga tgggacccag
     6541 gcctcgtcgc ccatcagcga cagctcccag accaccaccg aagggcctga ttcagctgtt
     6601 accccttcag acagttctga aattgtgtta gacggtaccg acaaccagta tttgggcctg
     6661 cagattggac agccccagga tgaagatgag gaagccacag gtattcttcc tgatgaagcc
     6721 tcggaggcct tcaggaactc ttccatggcc cttcaacagg cacatttatt gaaaaacatg
     6781 agtcactgca ggcagccttc tgacagcagt gttgataaat ttgtgttgag agatgaagct
     6841 actgaaccgg gtgatcaaga aaacaagcct tgccgcatca aaggtgacat tggacagtcc
     6901 actgatgatg actctgcacc tcttgtccat tgtgtccgcc ttttatctgc ttcgtttttg
     6961 ctaacagggg gaaaaaatgt gctggttccg gacagggatg tgagggtcag cgtgaaggcc
     7021 ctggccctca gctgtgtggg agcagctgtg gccctccacc cggaatcttt cttcagcaaa
     7081 ctctataaag ttcctcttga caccacggaa taccctgagg aacagtatgt ctcagacatc
     7141 ttgaactaca tcgatcatgg agacccacag gttcgaggag ccactgccat tctctgtggg
     7201 accctcatct gctccatcct cagcaggtcc cgcttccacg tgggagattg gatgggcacc
     7261 attagaaccc tcacaggaaa tacattttct ttggcggatt gcattccttt gctgcggaaa
     7321 acactgaagg atgagtcttc tgttacttgc aagttagctt gtacagctgt gaggaactgt
     7381 gtcatgagtc tctgcagcag cagctacagt gagttaggac tgcagctgat catcgatgtg
     7441 ctgactctga ggaacagttc ctattggctg gtgaggacag agcttctgga aacccttgca
     7501 gagattgact tcaggctggt gagctttttg gaggcaaaag cagaaaactt acacagaggg
     7561 gctcatcatt atacagggct tttaaaactg caagaacgag tgctcaataa tgttgtcatc
     7621 catttgcttg gagatgaaga ccccagggtg cgacatgttg ccgcagcatc actaattagg
     7681 cttgtcccaa agctgtttta taaatgtgac caaggacaag ctgatccagt agtggccgtg
     7741 gcaagagatc aaagcagtgt ttacctgaaa cttctcatgc atgagacgca gcctccatct
     7801 catttctccg tcagcacaat aaccagaata tatagaggct ataacctact accaagcata
     7861 acagacgtca ctatggaaaa taacctttca agagttattg cagcagtttc tcatgaacta
     7921 atcacatcaa ccaccagagc actcacattt ggatgctgtg aagctttgtg tcttctttcc
     7981 actgccttcc cagtttgcat ttggagttta ggttggcact gtggagtgcc tccactgagt
     8041 gcctcagatg agtctaggaa gagctgtacc gttgggatgg ccacaatgat tctgaccctg
     8101 ctctcgtcag cttggttccc attggatctc tcagcccatc aagatgcttt gattttggcc
     8161 ggaaacttgc ttgcagccag tgctcccaaa tctctgagaa gttcatgggc ctctgaagaa
     8221 gaagccaacc cagcagccac caagcaagag gaggtctggc cagccctggg ggaccgggcc
     8281 ctggtgccca tggtggagca gctcttctct cacctgctga aggtgattaa catttgtgcc
     8341 cacgtcctgg atgacgtggc tcctggaccc gcaataaagg cagccttgcc ttctctaaca
     8401 aacccccctt ctctaagtcc catccgacga aaggggaagg agaaagaacc aggagaacaa
     8461 gcatctgtac cgttgagtcc caagaaaggc agtgaggcca gtgcagcttc tagacaatct
     8521 gatacctcag gtcctgttac aacaagtaaa tcctcatcac tggggagttt ctatcatctt
     8581 ccttcatacc tcaaactgca tgatgtcctg aaagctacac acgctaacta caaggtcacg
     8641 ctggatcttc agaacagcac ggaaaagttt ggagggtttc ttcgctcagc cttggatgtt
     8701 ctttctcaga tactagagct ggccacactg caggacattg ggaagtgtgt tgaagagatc
     8761 ctaggatacc tgaaatcctg ctttagtcga gaaccaatga tggcaactgt ttgtgttcaa
     8821 caattgttga agactctctt tggcacaaac ttggcctccc agtttgatgg cttatcttcc
     8881 aaccccagca agtcacaagg ccgagcacag cgccttggct cctccagtgt gaggccaggc
     8941 ttgtaccact actgcttcat ggccccgtac acccacttca cccaggccct cgctgacgcc
     9001 agcctgagga acatggtgca ggcggagcag gagaacgaca cctcgggatg gtttgatgtc
     9061 ctccagaaag tgtctaccca gttgaagaca aacctcacga gtgtcacaaa gaaccgtgca
     9121 gataagaatg ctattcataa tcacattcgt ttgtttgaac ctcttgttat aaaagcttta
     9181 aaacagtaca cgactacaac atgtgtgcag ttacagaagc aggttttaga tttgctggcg
     9241 cagctggttc agttacgggt taattactgt cttctggatt cagatcaggt gtttattggc
     9301 tttgtattga aacagtttga atacattgaa gtgggccagt tcagggaatc agaggcaatc
     9361 attccaaaca tctttttctt cttggtatta ctatcttatg aacgctatca ttcaaaacag
     9421 atcattggaa ttcctaaaat cattcagctc tgtgatggca tcatggccag tggaaggaag
     9481 gctgtgacac atgccatacc ggctctgcag cccatagtcc acgacctctt tgtattaaga
     9541 ggaacaaata aagctgatgc aggaaaagag cttgaaaccc aaaaagaggt ggtggtgtca
     9601 atgttactga gactcatcca gtaccatcag gtgttggaga tgttcattct tgtcctgcag
     9661 cagtgccaca aggagaatga agacaagtgg aagcgactgt ctcgacagat agctgacatc
     9721 atcctcccaa tgttagccaa acagcagatg cacattgact ctcatgaagc ccttggagtg
     9781 ttaaatacat tatttgagat tttggcccct tcctccctcc gtccggtaga catgctttta
     9841 cggagtatgt tcgtcactcc aaacacaatg gcgtccgtga gcactgttca actgtggata
     9901 tcgggaattc tggccatttt gagggttctg atttcccagt caactgaaga tattgttctt
     9961 tctcgtattc aggagctctc cttctctccg tatttaatct cctgtacagt aattaatagg
    10021 ttaagagatg gggacagtac ttcaacgcta gaagaacaca gtgaagggaa acaaataaag
    10081 aatttgccag aagaaacatt ttcaaggttt ctattacaac tggttggtat tcttttagaa
    10141 gacattgtta caaaacagct gaaggtggaa atgagtgagc agcaacatac tttctattgc
    10201 caggaactag gcacactgct aatgtgtctg atccacatct tcaagtctgg aatgttccgg
    10261 agaatcacag cagctgccac taggctgttc cgcagtgatg gctgtggcgg cagtttctac
    10321 accctggaca gcttgaactt gcgggctcgt tccatgatca ccacccaccc ggccctggtg
    10381 ctgctctggt gtcagatact gctgcttgtc aaccacaccg actaccgctg gtgggcagaa
    10441 gtgcagcaga ccccgaaaag acacagtctg tccagcacaa agttacttag tccccagatg
    10501 tctggagaag aggaggattc tgacttggca gccaaacttg gaatgtgcaa tagagaaata
    10561 gtacgaagag gggctctcat tctcttctgt gattatgtct gtcagaacct ccatgactcc
    10621 gagcacttaa cgtggctcat tgtaaatcac attcaagatc tgatcagcct ttcccacgag
    10681 cctccagtac aggacttcat cagtgccgtt catcggaact ctgctgccag cggcctgttc
    10741 atccaggcaa ttcagtctcg ttgtgaaaac ctttcaactc caaccatgct gaagaaaact
    10801 cttcagtgct tggaggggat ccatctcagc cagtcgggag ctgtgctcac gctgtatgtg
    10861 gacaggcttc tgtgcacccc tttccgtgtg ctggctcgca tggtcgacat ccttgcttgt
    10921 cgccgggtag aaatgcttct ggctgcaaat ttacagagca gcatggccca gttgccaatg
    10981 gaagaactca acagaatcca ggaatacctt cagagcagcg ggctcgctca gagacaccaa
    11041 aggctctatt ccctgctgga caggtttcgt ctctccacca tgcaagactc acttagtccc
    11101 tctcctccag tctcttccca cccgctggac ggggatgggc acgtgtcact ggaaacagtg
    11161 agtccggaca aagactggta cgttcatctt gtcaaatccc agtgttggac caggtcagat
    11221 tctgcactgc tggaaggtgc agagctggtg aatcggattc ctgctgaaga tatgaatgcc
    11281 ttcatgatga actcggagtt caacctaagc ctgctagctc catgcttaag cctagggatg
    11341 agtgaaattt ctggtggcca gaagagtgcc ctttttgaag cagcccgtga ggtgactctg
    11401 gcccgtgtga gcggcaccgt gcagcagctc cctgctgtcc atcatgtctt ccagcccgag
    11461 ctgcctgcag aaccggcggc ctactggagc aagttgaatg atctgtttgg ggatgctgca
    11521 ctgtatcagt ccctgcccac tctggcccgg gccctggcac agtacctggt ggtggtctcc
    11581 aaactgccca gtcatttgca ccttcctcct gagaaagaga aggacattgt gaaattcgtg
    11641 gtggcaaccc ttgaggccct gtcctggcat ttgatccatg agcagatccc gctgagtctg
    11701 gatctccagg cagggctgga ctgctgctgc ctggccctgc agctgcctgg cctctggagc
    11761 gtggtctcct ccacagagtt tgtgacccac gcctgctccc tcatccactg tgtgcacttc
    11821 atcctggagg ccgttgcagt gcagcctgga gagcagcttc ttagtccaga aagaaggaca
    11881 aataccccaa aagccatcag cgaggaggag gaggaagtag atccaaacac acagaatcct
    11941 aagtatatca ctgcagcctg tgagatggtg gcagaaatgg tggagtctct gcagtcggtg
    12001 ttggccttgg gtcataaaag gaatagcggc gtgccggcgt ttctcacgcc attgctcagg
    12061 aacatcatca tcagcctggc ccgcctgccc cttgtcaaca gctacacacg tgtgccccca
    12121 ctggtgtgga agcttggatg gtcacccaaa ccgggagggg attttggcac agcattccct
    12181 gagatccccg tggagttcct ccaggaaaag gaagtcttta aggagttcat ctaccgcatc
    12241 aacacactag gctggaccag tcgtactcag tttgaagaaa cttgggccac cctccttggt
    12301 gtcctggtga cgcagcccct cgtgatggag caggaggaga gcccaccaga agaagacaca
    12361 gagaggaccc agatcaacgt cctggccgtg caggccatca cctcactggt gctcagtgca
    12421 atgactgtgc ctgtggccgg caacccagct gtaagctgct tggagcagca gccccggaac
    12481 aagcctctga aagctctcga caccaggttt gggaggaagc tgagcattat cagagggatt
    12541 gtggagcaag agattcaagc aatggtttca aagagagaga atattgccac ccatcattta
    12601 tatcaggcat gggatcctgt cccttctctg tctccggcta ctacaggtgc cctcatcagc
    12661 cacgagaagc tgctgctgca gatcaacccc gagcgggagc tggggagcat gagctacaaa
    12721 ctcggccagg tgtccataca ctccgtgtgg ctagggaaca gcatcacacc cctgagggag
    12781 gaggaatggg acgaggaaga ggaggaggag gccgacgccc ctgcaccttc gtcaccaccc
    12841 acgtctccag tcaactccag gaaacaccgg gctggagttg acatccactc ctgttcgcag
    12901 tttttgcttg agttgtacag ccgctggatc ctgccgtcca gctcagccag gaggaccccg
    12961 gccatcctga tcagtgaggt ggtcagatcc cttctagtgg tctcagactt gttcaccgag
    13021 cgcaaccagt ttgagctgat gtatgtgacg ctgacagaac tgcgaagggt gcacccttca
    13081 gaagacgaga tcctcgctca gtacctggtg cctgccacct gcaaggcagc tgccgtcctt
    13141 gggatggaca aggccgtggc ggagcctgtc agccgcctgc tggagagcac gctcaggagc
    13201 agccacctgc ccagcagggt tggagccctg cacggcatcc tctatgtgct ggagtgcgac
    13261 ctgctggacg acactgccaa gcagctcatc ccggtcatca gcgactatct cctctccaac
    13321 ctgaaaggga tcgcccactg cgtgaacatt cacagccagc agcacgtact ggtcatgtgt
    13381 gccactgcgt tttacctcat tgagaactat cctctggacg tagggccgga attttcagca
    13441 tcaataatac agatgtgtgg ggtgatgctg tctggaagtg aggagtccac cccctccatc
    13501 atttaccact gtgccctcag aggcctggag cgcctcctgc tctctgagca gctctcccgc
    13561 ctggatgcag aatcgctggt caagctgagt gtggacagag tgaacgtgca cagcccgcac
    13621 cgggccatgg cggctctggg cctgatgctc acctgcatgt acacaggaaa ggagaaagtc
    13681 agtccgggta gaacttcaga ccctaatcct gcagcccccg acagcgagtc agtgattgtt
    13741 gctatggagc gggtatctgt tctttttgat aggatcagga aaggctttcc ttgtgaagcc
    13801 agagtggtgg ccaggatcct gccccagttt ctagacgact tcttcccacc ccaggacatc
    13861 atgaacaaag tcatcggaga gtttctgtcc aaccagcagc cataccccca gttcatggcc
    13921 accgtggtgt ataaggtgtt tcagactctg cacagcaccg ggcagtcgtc catggtccgg
    13981 gactgggtca tgctgtccct ctccaacttc acgcagaggg ccccggtcgc catggccacg
    14041 tggagcctct cctgcttctt tgtcagcgcg tccaccagcc cgtgggtcgc ggcgatcctc
    14101 ccacatgtca tcagcaggat gggcaagctg gagcaggtgg acgtgaacct tttctgcctg
    14161 gtcgccacag acttctacag acaccagata gaggaggagc tcgaccgcag ggccttccag
    14221 tctgtgcttg aggtggttgc agccccagga agcccatatc accggctgct gacttgttta
    14281 cgaaatgtcc acaaggtcac cacctgcggt ggaagcggtg actacaaaga cgatgacgac
    14341 aagtaaaacc tctacttcca atcgcaccat catcaccatc accatcacca ccatgattac
    14401 aaggatgacg acgataagtg aggcggccgc caacttgata tcaagcttat cgataatcaa
    14461 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
    14521 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
    14581 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
    14641 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
    14701 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
    14761 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
    14821 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
    14881 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
    14941 gcggaccttc cttcccgcgg gcgcccccct cctcacggcg agcgaagctt gtcgagaagt
    15001 actagaggat ctctagagcc tgcagtctcg acaagcttgt cgagaagtac tagaggatca
    15061 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    15121 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    15181 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    15241 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
    15301 cactgcttga gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg
    15361 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
    15421 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
    15481 tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact
    15541 ccatgtgaca aaccgtcatc ttcggctact tt
//
