LOCUS       sequence-216048-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Minhas GS, Bawdon D, Herman R, Rudden M, Stone AP, James AG, Thomas 
            GH, Newstead S
  TITLE     Structural basis of malodour precursor transport in the human 
            axilla.
  JOURNAL   Elife. 2018 Jul 3;7. pii: 34995. doi: 10.7554/eLife.34995.
  PUBMED    29966586
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-3"; volume: "7. pii: 34995. doi"; pages: " 
            10.7554/eLife.34995"
COMMENT     Sequence Label: pWaldo-GFPe-SH1446
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      573..582
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agaaggagac tcgacatggc aacaaataac tcccatgaac aaacaattca atcaatacca
       61 cagaaaggat ttttcggtca tcctagggga ctaggtgtgc tctttttcgt agagttttgg
      121 gaaagattca gttactacgg tatgcgtgcg atgctcatat tttatatgta tttcgcaata
      181 caccagaatg gattaggtat tgataaaact acagcaatgt caattatgtc agtatatgga
      241 gcactgattt acatgtcttc tattcctggc gcatggattg ctgatagaat tacaggcaca
      301 cgtggtgcta ctttattagg tgctgtactt attattattg gacatatctg tttaagttta
      361 ccattcgcct tatttggttt attctcatca atgttcttta ttattatcgg atcaggttta
      421 atgaaaccta atatttcaaa tattgtaggt cgtttatatc ctgaaaatga tacacgtatt
      481 gatgcaggtt tcgttatttt ctatatgtct gttaacttag gtgcattaat ttcacctatc
      541 atactgcaac attttgtaga tatacgtaat ttccatggtg gcttccttct tgcagctatt
      601 ggtatggcat taggtcttgt ttggtattta ttatttaata gaaaaaattt aggtagtgtt
      661 ggaatgaaac ctacgaatcc actatctaaa gaagaaaagc gtaaatatgg aatgattatc
      721 ggtatcatag tagcaattgt gantgtcgta ttattagtta catattatac ccatacacnt
      781 tcctttcgat ttaattagta atactgtatt agttctagga gtagctttta ccaatcattt
      841 actttacact atgcttcgta gtaaagacgt taccgatggg agaacgntct cgagtaa
//
