LOCUS       sequence-21605-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Henry GL, Melton DA
  TITLE     Mixer, a homeobox gene required for endoderm development.
  JOURNAL   Science. 1998 Jul 3. 281(5373):91-6.
  PUBMED    9651252
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1998 Jul 3. 281(5373):91-6."
COMMENT     Sequence Label: pCS300 (DM#184)
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    64..198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(88..107)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     142..161
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(312..332)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(312..330)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(351..367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(351..367)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(364..386)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    375..391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(399..405,406..423,424..429))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    444..465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(582..599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atgtctttgc ngaccctcga ggattcgcgg ccgctctaga actatagtga gtcgtattac
       61 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      121 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      181 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
      241 gaggtgtggg aggtttttta attcgcggcc aggcgcgccg gcgccgttta aacggtaccc
      301 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
      361 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata
      421 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      481 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      541 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      601 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      661 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      721 aggaaccgta aaaggccgcg ttgcggcgtt ttccntaggc tcgccccctg aaaacccnna
      781 aatcgacctc antgaaagtg cnaaccnacn gataaaaaac ngggtncccn gaacccnggg
      841 cccccntcac tgcntacgan ctgccctncn tcganggc
//
