LOCUS       sequence-216050-       10054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Kuo MY, Chao M, Taylor J
  TITLE     Initiation of replication of the human hepatitis delta virus genome 
            from cloned DNA: role of delta antigen.
  JOURNAL   J Virol. 1989 May . 63(5):1945-50.
  PUBMED    2649689
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1989 May . 63(5):1945-50."
COMMENT     Sequence Label: pSVL(D3)
FEATURES             Location/Qualifiers
     source          1..10054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1276..1295
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     polyA_signal    1460..1594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1497..1516)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1551..1570
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(6655..6673)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6656..6673)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6681..6698)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6681..6697)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6690..6712)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        7997..8354
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7997..8188
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8020..8040)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8205..8340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8833..8851)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8919..9023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9024..9092,9093..9884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9242..9261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gtttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc
      661 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag
      721 aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc
      781 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
      841 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
      901 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
      961 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
     1021 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
     1081 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
     1141 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
     1201 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     1261 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     1321 ggtgatgccg gccacgatgc gtccggcgta gaggatcata atcagccata ccacatttgt
     1381 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     1441 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     1501 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1561 caaactcatc aatgtatctt atcatgtctg gatccccggg taccgagctc gaattctcgg
     1621 aaagaaggat aaggatggag agggggctcc cccggcgaag agggcccgaa cggaccagat
     1681 ggaggtagac tccggacctg ggaagaggcc tctcagggga ggattcaccg acaaggagag
     1741 gcaggatcac cgacgaagga aggccctcga gaacaagaag aagcagctat cggcgggagg
     1801 caagaacctc agcaaggagg aagaagagga actcaggagg ttgaccgagg aagacgagag
     1861 aagggaaaga agagtagccg gcccgccggt tgggggtgtg aaccccctcg aaggtggatc
     1921 gaggggagcg cccgggggcg gcttcgtccc caatctgcag ggagtcccgg agtccccctt
     1981 ctctcggacc ggggaggggc tggacatcag gggaaaccag ggatttccat aggatatact
     2041 cttcccagcc gatccgccct tttctcccca gagttgtcga ccccagtgaa taaagcgggt
     2101 ttccactcac aggtttgcgt ctcgcgtcct tctttcctct tcgggtcggc atggcatctc
     2161 cacctcctcg cggtccgacc tgggcatccg aaggaggacg cacgtccact cggatggcta
     2221 agggagagcc acttttctct cgattctcta tcggaatcta gagagatttg tgggtcccat
     2281 tcgccattac cgaggggacg gtcccctcgg aatgttgccc agccggcgcc agcgaggagg
     2341 ctgggaccat gccggccatc aggtaagaaa ggatggaacg cggaccctgc agagtggggt
     2401 cccgccattc ctgggcgacc cttggggggg gagtcggaat cgagcatcgg gaagggcatc
     2461 ccatggctcc actggtcccc ggtgttccca gcaccccctc cggtcacttt cgaagggggt
     2521 ccggggtccc gctagatggg gacgataagt cgagttcccc gggataagcc tcactcgtcc
     2581 cctctcgggg ggcggaacac ccaccggcta gccccgttgc tttctttgct ttcctcctcg
     2641 cttcggtctc cccctactcc tagcatctcc tcctatcgct atggtcttac tcctaccgct
     2701 cgaagcgcct cttgttcgct gaaggggtcc tctggaggtg atttctctgc tcatctccga
     2761 gtggattcct ccctctgagt gctactcaac ccttcgggcc ggagtgctct ccagatctgg
     2821 agattgggcc cctcggatcg ggaggcccgc ccctttttct tccaccttcc tctttcccct
     2881 tccagagatt ctccggcgtt gtggggatct cgtccgcttc ctaagacctc tctttgcttc
     2941 ttgggagtag tttctccgac gttccaatgc tctttaccgt gacatcccct ctcgggagct
     3001 gatcctcccc ccgcgtctcc tcgctcggaa cttggctcag gctcgggctc gggcggcgag
     3061 tccagcagtc tcctctttac agaaaagagt aagagtactg aggactgccg cctctagccg
     3121 agatgagccg gtccgagtcg aggaagaacc gcggagggag agaagagatc ctcgagcagt
     3181 gggtggccgg aagaaagaag ttagaggaac tcgagagaga cctccggaag acaaagaaga
     3241 aactcaagaa gatagaggac gaaaatccct ggctggggaa catcaaagga attctcggaa
     3301 agaaggataa ggatggagag ggggctcccc cggcgaagag ggcccgaacg gaccagatgg
     3361 aggtagactc cggacctggg aagaggcctc tcaggggagg attcaccgac aaggagaggc
     3421 aggatcaccg acgaaggaag gccctcgaga acaagaagaa gcagctatcg gcgggaggca
     3481 agaacctcag caaggaggaa gaagaggaac tcaggaggtt gaccgaggaa gacgagagaa
     3541 gggaaagaag agtagccggc ccgccggttg ggggtgtgaa ccccctcgaa ggtggatcga
     3601 ggggagcgcc cgggggcggc ttcgtcccca atctgcaggg agtcccggag tcccccttct
     3661 ctcggaccgg ggaggggctg gacatcaggg gaaaccaggg atttccatag gatatactct
     3721 tcccagccga tccgcccttt tctccccaga gttgtcgacc ccagtgaata aagcgggttt
     3781 ccactcacag gtttgcgtct cgcgtccttc tttcctcttc gggtcggcat ggcatctcca
     3841 cctcctcgcg gtccgacctg ggcatccgaa ggaggacgca cgtccactcg gatggctaag
     3901 ggagagccac ttttctctcg attctctatc ggaatctaga gagatttgtg ggtcccattc
     3961 gccattaccg aggggacggt cccctcggaa tgttgcccag ccggcgccag cgaggaggct
     4021 gggaccatgc cggccatcag gtaagaaagg atggaacgcg gaccctgcag agtggggtcc
     4081 cgccattcct gggcgaccct tgggggggga gtcggaatcg agcatcggga agggcatccc
     4141 atggctccac tggtccccgg tgttcccagc accccctccg gtcactttcg aagggggtcc
     4201 ggggtcccgc tagatgggga cgataagtcg agttccccgg gataagcctc actcgtcccc
     4261 tctcgggggg cggaacaccc accggctagc cccgttgctt tctttgcttt cctcctcgct
     4321 tcggtctccc cctactccta gcatctcctc ctatcgctat ggtcttactc ctaccgctcg
     4381 aagcgcctct tgttcgctga aggggtcctc tggaggtgat ttctctgctc atctccgagt
     4441 ggattcctcc ctctgagtgc tactcaaccc ttcgggccgg agtgctctcc agatctggag
     4501 attgggcccc tcggatcggg aggcccgccc ctttttcttc caccttcctc tttccccttc
     4561 cagagattct ccggcgttgt ggggatctcg tccgcttcct aagacctctc tttgcttctt
     4621 gggagtagtt tctccgacgt tccaatgctc tttaccgtga catcccctct cgggagctga
     4681 tcctcccccc gcgtctcctc gctcggaact tggctcaggc tcgggctcgg gcggcgagtc
     4741 cagcagtctc ctctttacag aaaagagtaa gagtactgag gactgccgcc tctagccgag
     4801 atgagccggt ccgagtcgag gaagaaccgc ggagggagag aagagatcct cgagcagtgg
     4861 gtggccggaa gaaagaagtt agaggaactc gagagagacc tccggaagac aaagaagaaa
     4921 ctcaagaaga tagaggacga aaatccctgg ctggggaaca tcaaaggaat tctcggaaag
     4981 aaggataagg atggagaggg ggctcccccg gcgaagaggg cccgaacgga ccagatggag
     5041 gtagactccg gacctgggaa gaggcctctc aggggaggat tcaccgacaa ggagaggcag
     5101 gatcaccgac gaaggaaggc cctcgagaac aagaagaagc agctatcggc gggaggcaag
     5161 aacctcagca aggaggaaga agaggaactc aggaggttga ccgaggaaga cgagagaagg
     5221 gaaagaagag tagccggccc gccggttggg ggtgtgaacc ccctcgaagg tggatcgagg
     5281 ggagcgcccg ggggcggctt cgtccccaat ctgcagggag tcccggagtc ccccttctct
     5341 cggaccgggg aggggctgga catcagggga aaccagggat ttccatagga tatactcttc
     5401 ccagccgatc cgcccttttc tccccagagt tgtcgacccc agtgaataaa gcgggtttcc
     5461 actcacaggt ttgcgtctcg cgtccttctt tcctcttcgg gtcggcatgg catctccacc
     5521 tcctcgcggt ccgacctggg catccgaagg aggacgcacg tccactcgga tggctaaggg
     5581 agagccactt ttctctcgat tctctatcgg aatctagaga gatttgtggg tcccattcgc
     5641 cattaccgag gggacggtcc cctcggaatg ttgcccagcc ggcgccagcg aggaggctgg
     5701 gaccatgccg gccatcaggt aagaaaggat ggaacgcgga ccctgcagag tggggtcccg
     5761 ccattcctgg gcgacccttg gggggggagt cggaatcgag catcgggaag ggcatcccat
     5821 ggctccactg gtccccggtg ttcccagcac cccctccggt cactttcgaa gggggtccgg
     5881 ggtcccgcta gatggggacg ataagtcgag ttccccggga taagcctcac tcgtcccctc
     5941 tcggggggcg gaacacccac cggctagccc cgttgctttc tttgctttcc tcctcgcttc
     6001 ggtctccccc tactcctagc atctcctcct atcgctatgg tcttactcct accgctcgaa
     6061 gcgcctcttg ttcgctgaag gggtcctctg gaggtgattt ctctgctcat ctccgagtgg
     6121 attcctccct ctgagtgcta ctcaaccctt cgggccggag tgctctccag atctggagat
     6181 tgggcccctc ggatcgggag gcccgcccct ttttcttcca ccttcctctt tccccttcca
     6241 gagattctcc ggcgttgtgg ggatctcgtc cgcttcctaa gacctctctt tgcttcttgg
     6301 gagtagtttc tccgacgttc caatgctctt taccgtgaca tcccctctcg ggagctgatc
     6361 ctccccccgc gtctcctcgc tcggaacttg gctcaggctc gggctcgggc ggcgagtcca
     6421 gcagtctcct ctttacagaa aagagtaaga gtactgagga ctgccgcctc tagccgagat
     6481 gagccggtcc gagtcgagga agaaccgcgg agggagagaa gagatcctcg agcagtgggt
     6541 ggccggaaga aagaagttag aggaactcga gagagacctc cggaagacaa agaagaaact
     6601 caagaagata gaggacgaaa atccctggct ggggaacatc aaaggaattc gtattctata
     6661 gtgtcaccta aatccaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6721 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagggg tctagactcg
     6781 agggatcgat ccggtttaga gcagaagtaa cacttccgta caggcctaga agtaaaggca
     6841 acatccactg aggagcagtt ctttgatttg caccaccagg agcctcaaat ttttcaataa
     6901 attcacctga ctgcacatta ggactttggc tttgagcttc ccacctctca gttacttgct
     6961 gaatactgtc tgcttcatca atattatcat aggtgtgccc aaatgatatt tgcaaccctt
     7021 ccctgttggc tacttgtctc accattgtag gcctaatggg agacaaagta gagtagtaat
     7081 cttgtaaaga gttataccaa ttaacaggag cattaattac tgtccaagta gtttcctcta
     7141 aaaaccttgc caaactgtcc cttaaatatc tttgggttct tctttcaagc tcctgtgagg
     7201 tgagcctagg aatgtcattt tgtattacac gccaaaaagc ttgagaaatg gcattaaaaa
     7261 gtgttggacc ccaatgtctg gggtcaagat actgaacact gtgaacaaag gtttgtactc
     7321 caggaaataa aatatcataa taatcatctg gcctatacaa atctacagcc attcctggtt
     7381 gttgatataa accaacagta gaaactttgt gatcccagtc actaaaaaat ctatacccca
     7441 cttgagcaac agcgctcaca ccagtcacag tttgcagtaa agctgcaaat ccagctatag
     7501 cagcaggagc cccagatatc acagcatagg cctgtggagt gaggcctata gcagcaattg
     7561 cctcagaggt tgttaggcct tcaacagtag caacagatgc aagttgcact tcaattgcag
     7621 cagcggcctc tccagcagca atttcagcta ctgaaaatcc agtagcagca gcagcttcag
     7681 acacagtagc aattaggtcc cccaacagtg ttaaagcagc acccatggac ctgaaataaa
     7741 agacaaaaag actaaactta ccagttaact ttctggtttt tcagttaacc tttctggttt
     7801 tttgcgtttc ccgtcaacag tatcttcccc ttcacaaaat tgcagcaaaa gctctaaaac
     7861 aaacacaaaa aggcgttgag ctgttttttt actttcagtc catgacctac gaaccttaac
     7921 ggaggcctgg cgtgacagcc ggcgcagcac catggcctga aataacctct gaaagaggaa
     7981 cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     8041 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8101 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8161 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     8221 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8281 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8341 taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
     8401 cagctaatgg accttctagg tcttgaaagg agtgcctggg ggaatattcc tctgatgaga
     8461 aaggcatatt taaaaaaatg caaggagttt catcctgata aaggaggaga tgaagaaaaa
     8521 atgaagaaaa tgaatactct gtacaagaaa atggaagatg gagtaaaata tgctcatcaa
     8581 cctgactttg gaggcttctg ggatgcaact gaggtatttg cttcttcctt aaatcctggt
     8641 gttgatgcaa tgtactgcaa acaatggcct gagtgtgcaa agaaaatgtc tgctaactgc
     8701 atatgcttgc tgtgcttact gaggatgaag catgaaaata gaaaattata caggaaagat
     8761 ccacttgtgt gggttgattg ctactgcttc gatgataagc tgtcaaacat gagaattctt
     8821 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     8881 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8941 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     9001 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9061 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9121 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9181 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9241 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     9301 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9361 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9421 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9481 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9541 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     9601 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9661 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9721 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9781 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9841 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9901 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9961 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10021 cgtcagaccc cgtagaaaag atcaaaggat cttc
//
