LOCUS       sequence-21611-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Murtaugh LC, Stanger BZ, Kwan KM, Melton DA
  TITLE     Notch signaling controls multiple steps of pancreatic 
            differentiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5.
  PUBMED    14657333
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Dec 9. 100(25):14920-5."
COMMENT     Sequence Label: pBTG (pBigT-IRES-GFP; DM#268)
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     124..140
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(175..208)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    237..371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    491..625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg ggggagggag
       61 aggggcggat cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgatcga
      121 cctcgaggtc gacggtatcg ataagctagc ttgggctgca ggtcgaggga cctaataact
      181 tcgtatagca tacattatac gaagttatat taagggttcc ggatcagctt gatggggatc
      241 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
      301 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      361 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
      421 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
      481 tagagtcgca gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
      541 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      601 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
      661 tcagggggag gtgtgggagg ttttt
//
