LOCUS       sequence-21614-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Murtaugh LC, Stanger BZ, Kwan KM, Melton DA
  TITLE     Notch signaling controls multiple steps of pancreatic 
            differentiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5.
  PUBMED    14657333
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Dec 9. 100(25):14920-5."
COMMENT     Sequence Label: pBS mNotch1-IC (DM#270)
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..27
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     12..28
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             77..100
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggccccccc tcgaggtcga cggtatcgat aagcttgata attctagcta gctagccggc
       61 ccaattcggt accatggact acaaagacga tgacgataaa gcccggcggc agcatggcca
      121 gctctggttc cctgagggtt tcaaagtgtc agaggccagc aagaagaagc ggagagagcc
      181 cctcggcgag gactcagtcg gcctcaagcc cctgaagaat gcctcagatg gtgctctgat
      241 ggacgacaat cagaacgagt ggggagacga agacctggag accaagaagt tccggtttga
      301 ggagccagta gttctccctg acctgagtga tcagactgac cacagacagt ggacccagca
      361 gcacctggac gctgctgacc tgcgcatgtc tgccatggcc ccaacaccgc ctcaggggga
      421 ggtggatgct gactgcatgg atgtcaatgt tcgaggacca gatggcttca cacccctcat
      481 gattgcctcc tgcagtggag ggggccttga gacaggcaac agtgaagaag aagaagatgc
      541 acctgctgtc atctctgact tcatctacca gggcgccagc ttgcacaacc agacagaccg
      601 caccggggag accgccttgc acttggctgc ccgatactct cgttcagatg ctgcaaagcg
      661 cttgctggag gccagtgcag atgccaacat ccaggacaac atgggccgta ctccgttaca
      721 tgcagcagtt tctgcagatg ctcagggtgt cttccagatc ctgctccgga acagggccac
      781 agatctggat gcccgaatgc atgatggcac aact
//
