LOCUS       sequence-21625-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Rajagopal J, Anderson WJ, Kume S, Martinez OI, Melton DA
  TITLE     Insulin staining of ES cell progeny from insulin uptake.
  JOURNAL   Science. 2003 Jan 17. 299(5605):363.
  PUBMED    12532008
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2003 Jan 17. 299(5605):363."
COMMENT     Sequence Label: Pdx1-RFP hygro (DM#236)
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..51)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    61..142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     86..105
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(329..352)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(527..546)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgcgnggcg gccgctctag ttagatacat tgatgagttt ggacaaacca caactagaat
       61 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      121 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      181 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
      241 ttatgatcta gagtcgcggc cggccgctac aggaacaggt ggtggcggcc ctcggtgcgc
      301 tcgtactgct ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag cttggagtcc
      361 acgtagtagt agccgggcag ctgcacgggc ttcttggcca tgtagatgga cttgaactcc
      421 accaggtagt ggccgccgtc cttcagcttc agggccttgt ggatctcgcc cttcagcacg
      481 ccgtcgcggg ggtacaggcg ctcggtggag gcctcccagc ccatggtctt cttctgcatt
      541 acggggccgt cggaggggaa gttcacgccg atgaacttca ccttgtagat gaagcagccg
      601 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
      661 cgctcccact tgaagccctc ggggaaggac agcttcttgt agtcggggat gtcggcgggg
      721 tgcttccntt acntaaancn tgntaccgna tggaactggg gggga
//
