LOCUS       sequence-216259-       11580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic Ras activation system; cytoplasmic component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11580)
  AUTHORS   Toettcher JE, Weiner OD, Lim WA
  TITLE     Using optogenetics to interrogate the dynamic control of signal 
            transmission by the ras/erk module.
  JOURNAL   Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
  PUBMED    24315106
REFERENCE   2  (bases 1 to 11580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.11"; journalName: "Cell"; date: "2013-12-5- 5";
            volume: "155"; issue: "6"; pages: "1422-34"
COMMENT     Sequence Label: pHR SFFVp YFP-PIF-SOS2cat
FEATURES             Location/Qualifiers
     source          1..11580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..119
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(189..209)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        194..523
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      374..509
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1657..1722
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1852..1872
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2297..2431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2334..2353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2388..2407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2599..3232
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3279..3404
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3901..4134
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4319..4363
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4648..4765
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4918..5325
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(5401..5403,5404..5406,5407..6117)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5446..5467)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5707..5726)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6054..6075
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8042..8630
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8095..8115)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8513..8524)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8633..8649)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8634..8650)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8740..8973
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8998..9016)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8999..9016)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9306..9325)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9539..9558)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9658..9680
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9718..9736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9804..9908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9909..9977,9978..10769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10127..10146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10940..11528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11429..11448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
       61 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      121 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      181 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
      241 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
      301 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
      361 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
      421 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
      481 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
      541 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
      601 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
      661 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
      721 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
      781 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
      841 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
      901 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
      961 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
     1021 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
     1081 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
     1141 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
     1201 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
     1261 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
     1321 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
     1381 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
     1441 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
     1501 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
     1561 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     1621 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     1681 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     1741 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     1801 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     1861 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     1921 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     1981 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
     2041 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
     2101 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
     2161 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
     2221 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2281 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2341 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2401 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     2461 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     2521 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     2581 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     2641 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     2701 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     2761 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     2821 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     2881 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     2941 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     3001 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     3061 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3121 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3181 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     3241 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     3301 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     3361 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     3421 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     3481 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     3541 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     3601 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     3661 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     3721 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     3781 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     3841 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     3901 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     3961 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     4021 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     4081 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     4141 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     4201 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     4261 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     4321 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     4381 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     4441 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     4501 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     4561 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     4621 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     4681 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     4741 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     4801 gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat tttatttagt
     4861 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agctgcagta
     4921 acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg
     4981 gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg agcagtttcg
     5041 gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc ccgaggccaa
     5101 gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc catcagatgt
     5161 ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta accaatcagc
     5221 ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag agctcacaac
     5281 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga tctggagctc
     5341 tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc gcttggtacc
     5401 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5461 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     5521 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5581 ctcgtgacca ccttcggcta cggcctgaag tgcttcgccc gctaccccga ccacatgaag
     5641 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5701 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5761 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5821 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     5881 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     5941 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6001 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6061 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggga
     6121 tccatgatgt tcttaccaac cgattattgt tgcaggttaa gcgatcaaga gtatatggag
     6181 cttgtgtttg agaatggcca gattcttgca aagggccaaa gatccaacgt ttctctgcat
     6241 aatcaacgta ccaaatcgat catggatttg tatgaggcag agtataacga ggatttcatg
     6301 aagagtatca tccatggtgg tggtggtgcc atcacaaatc tcggggacac gcaggttgtt
     6361 ccacaaagtc atgttgctgc tgcccatgaa acaaacatgt tggaaagcaa taaacatgtt
     6421 gacagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg tgaattggcg
     6481 gcccaaccac tgagattacc aagtcctgaa gtatatcgtt ttgtagtaaa agactctgag
     6541 gaaaacattg tttttgaaga caacttgcaa agtagaagtg gcatccccat tattaaagga
     6601 ggaactgtag tgaaattaat tgaaaggtta acatatcata tgtatgcaga tcccaatttt
     6661 gttcgtactt ttcttaccac atatcgttca ttttgtaaac cacaggaatt gctgagctta
     6721 ctgattgaac ggtttgaaat tccagagcca gaacctactg acgcagacaa attggcaata
     6781 gagaaaggcg agcagccaat cagtgcagac cttaaaagat ttcgcaagga atatgtccaa
     6841 ccagtacaac ttaggatctt aaatgtattt cggcattggg ttgaacatca tttttatgac
     6901 tttgaaagag acttggaatt gcttgaaaga ctagaatcct tcatttcaag tgtaagaggg
     6961 aaagctatga aaaaatgggt agagtcaatt gctaagatca tcaggaggaa gaagcaagct
     7021 caggcaaacg gagtaagcca taatattacc tttgaaagtc cacctccacc aattgaatgg
     7081 catatcagca aaccaggaca gtttgaaaca tttgatctca tgacacttca tccaatagaa
     7141 attgcacgtc agctgacact tttggagtct gatctttaca ggaaagttca accgtctgaa
     7201 cttgtaggga gtgtgtggac caaagaagat aaagaaataa attctccaaa tttattaaaa
     7261 atgattcgcc ataccacaaa tctcaccctc tggtttgaaa aatgcattgt ggaagcagaa
     7321 aattttgaag aacgggtggc agtactaagt agaattatag aaattctgca agtttttcaa
     7381 gatttgaata atttcaatgg cgtattggag atagtcagtg cagtaaattc agtgtcagta
     7441 tacagactag accatacctt tgaggcactg caggaaagga aaaggaaaat tttggacgaa
     7501 gctgtggaat taagtcaaga tcactttaaa aaatacctag taaaacttaa gtcaatcaat
     7561 ccaccttgtg tgcctttttt tggaatatat ttaacaaata ttctgaagac cgaagaaggg
     7621 aataatgatt ttttaaaaaa gaaagggaaa gatttaatca atttcagtaa gaggaggaaa
     7681 gtagctgaaa ttactggaga aattcagcag tatcagaatc agccttactg tttacggata
     7741 gaaccagata tgaggagatt ctttgaaaac cttaacccca tgggaagtgc atctgaaaaa
     7801 gagtttacag attatttgtt caacaagtca ctagaaattg aacctcgaaa ctgcaaacag
     7861 ccacctcgat ttcctaggaa atcaactttt tccttaaaat ctcctggaat aaggcctaac
     7921 acaggccgac atggctctac ctaagcggcc gcaactccca cctgcaacat gcgtgactga
     7981 ctgaggccgc gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga
     8041 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     8101 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     8161 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     8221 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     8281 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     8341 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     8401 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     8461 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8521 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8581 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     8641 tcgacctcga gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     8701 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     8761 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8821 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8881 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8941 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
     9001 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
     9061 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
     9121 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
     9181 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
     9241 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     9301 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     9361 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     9421 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     9481 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
     9541 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     9601 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     9661 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     9721 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     9781 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10321 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
//
