LOCUS       sequence-216264-       11457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of flag tagged GREB1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Haines CN, Braunreiter KM, Mo XM, Burd CJ
  TITLE     GREB1 isoforms regulate proliferation independent of ERalpha 
            co-regulator activities in breast cancer.
  JOURNAL   Endocr Relat Cancer. 2018 Jul;25(7):735-746. doi: 
            10.1530/ERC-17-0496. Epub 2018 Apr 25.
  PUBMED    29695586
REFERENCE   2  (bases 1 to 11457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1530/ERC-17-0496"; journalName: "Endocr Relat Cancer"; date: 
            "2018-07"; volume: "25"; issue: "7"; pages: "735-746"
COMMENT     Sequence Label: pcDNA-3xflagGREB1a
FEATURES             Location/Qualifiers
     source          1..11457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      662..671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             671..736
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(6645..6662)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6651..6875
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6921..7349
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7008..7027)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7218..7239
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7358..7378)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7363..7692
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7543..7678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7605..7624
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7741..8766
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7741..8766
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8896..9017
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8933..8952)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8987..9006
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9066..9082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9066..9082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9079..9101)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9090..9106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9114..9120,9121..9138,9139..9144))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9159..9180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9297..9314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9468..10056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9548..9567)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10227..11018,11019..11087))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10850..10869
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11088..11192)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11267..11286)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 ggccaccatg gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa
      721 ggatgacgat gacaagctag cgtttaaact taagcttggt accatgggaa attcttacgc
      781 tggacagctg aagacgacac gctttgaaga ggtcttgcac aattccatcg aggcatccct
      841 gcggtccaac aacctggtgc ccaggcccat cttttcccag ctgtacctgg aagctgagca
      901 gcagcttgcc gctctagaag gtggtagccg agtggacaat gaggaagagg aagaagaggg
      961 agaaggaggg ctggaaacaa atggcccccc aaaccctttc cagctgcacc ctctgcctga
     1021 aggatgctgt accacagacg ggttttgcca ggccgggaag gacctgcgcc ttgtctccat
     1081 ttccaacgag cccatggatg tccctgcggg ctttctcctc gtgggggtca agtcccccag
     1141 cctgccggac catctcctgg tgtgcgccgt tgacaagagg ttcttgccag atgacaatgg
     1201 ccacaatgct cttcttggtt tctctgggaa ttgtgttggc tgtggaaaga aaggcttctg
     1261 ttacttcacg gaattctcca atcatataaa tctgaaactg accactcaac ccaagaagca
     1321 gaaacacttg aagtattacc tggtccgtaa tgcacaaggg actctaacca aaggaccttt
     1381 aatctgttgg aaaggctcag agtttagaag ccggcagatc cccgccagta cttgttccag
     1441 ttccctcttc ccagccctgg agagcacggc tgccttcccc agcgagcccg ttcctgggac
     1501 gaaccccagc atcctgatgg gagctcagca ggcaggacca gcttctgatc acccctcact
     1561 aaacgcagca atgggtccgg ctgttttcaa cggcaaagat tccccgaagt gccaacaact
     1621 ggcaaagaat aacctgttgg ccctgccgcg accatcggct ttaggtatct tgtcaaactc
     1681 cgggcccccc aaaaaacgcc acaaagggtg gtctccagaa tctccatcag ctccagatgg
     1741 tggctgcccc caaggtggtg ggaacagagc taagtatgag agcgcaggca tgtcctgcgt
     1801 gccgcaggtt ggcttggtgg gaccagcttc agtcaccttt ccagtggtgg cctctggaga
     1861 accagtgtct gttcctgaca acttgctgaa aatatgcaag gccaagccag tgatatttaa
     1921 aggccatggg aacttccctt acctctgtgg gaacctgaat gacgtcgtgg tcagccccct
     1981 cttgtacacg tgctaccaga attcccagtc tgtctcacgg gcatacgagc agtacggcgc
     2041 ctctgccatc cagcccatct ccgaggagat gcagctcctg cttaccgtct actacctggt
     2101 ccagctggcc gcggaccagg tgcccttgat ggaggacctg gagcagatct tcctgcgctc
     2161 ttggcgcgag tcgcacctga ccgagatccg gcagtaccag caggcgccgc cgcagccctt
     2221 cccgcccgcg cccagcgccg cggcacccgt gacctccgcg cagctgccct ggctggccag
     2281 cctggccgcc agctcctgca acgacagcgt gcacgtcatc gagtgtgctt actccctggc
     2341 cgagggcctc tccgagatgt tccggctgtt ggtcgagggc aagcttgcca agaccaacta
     2401 cgtggtcatc atctgcgcct gccgcagcgc ggccatcgac tcctgcatcg ccgtcaccgg
     2461 aaaataccaa gcccggattc tttccgagag ccttctcact cctgcggagt accagaagga
     2521 agtcaattac gagctggtta cggggaaggt agactcgctg ggggccttct ttagcaccct
     2581 ctgtccagag ggtgacattg acattttgct ggacaaattt caccaggaaa atcaaggcca
     2641 tatttcttcc tcactcgctg cctcttctgt cactaaagca gcatccctgg atgtcagtgg
     2701 gacaccggtg tgcacaagtt acaatctgga gccacacagc atccggccct tccagctggc
     2761 agtagcgcag aagctcctct cccatgtgtg ttccattgcg gattccagca cccaaaatct
     2821 ggacctggga tcctttgaga aggtggactt tctcatttgc attcccccct cagaagtgac
     2881 ctaccagcag actctgctcc acgtgtggca ttcaggggtt ttgctggagc ttggtctgaa
     2941 gaaagagcac atgacgaagc agagggtgga acagtatgtt ctgaagctag acacggaggc
     3001 acagacaaaa tttaaggctt ttctgcaaaa ctccttccag aacccgcata cactttttgt
     3061 cctaatccat gaccatgcgc actgggatct tgtgagtagc actgttcata acctctattc
     3121 tcaaagtgac ccgtcggtgg gattggtgga ccgattgctc aactgcaggg aggtgaagga
     3181 ggcccccaac attgtgacac ttcacgtgac ctccttcccg tatgcactgc agacacagca
     3241 caccctcatc agcccctaca acgagatcca ctggcctgcc tcctgcagta atggagtgga
     3301 cttatatcat gaaaataaga agtacttcgg gctgtcggag tttattgaat ccaccctttc
     3361 aggacacagc ctccccttgc tcagatacga tagctccttt gaggccatgg tcactgcatt
     3421 aggaaaaagg ttcccccgcc tgcacagcgc ggtgatcagg acctttgttc tcgtgcagca
     3481 ctacgcggcc gccctgatgg ccgtaagcgg cctcccgcag atgaagaact acacgtcggt
     3541 ggagacgctg gagatcacgc agaacctcct caactccccg aagcagtgcc cctgcggcca
     3601 cgggctcatg gtcctgctgc gggtgccctg ttcgcccctg gcggtggtgg cctatgagcg
     3661 gctggcccac gtgcgggccc ggctggcgct ggaggagcac tttgagatca tcctgggcag
     3721 tcccagctca ggcgtcaccg tggggaagca cttcgtaaag cagctcagga tgtggcagaa
     3781 aatcgaggat gtggagtgga gaccccagac ttacttggag ctggagggtc tgccttgcat
     3841 cctgatcttc agtgggatgg acccgcatgg ggagtccttg ccgaggtctt tgaggtactg
     3901 tgacctgcga ttgataaact cctcctgctt ggtgagaaca gccttggagc aggagctggg
     3961 cctggctgcc tactttgtga gcaacgaggt tcccttggag aagggggcta ggaacgaggc
     4021 cttggagagt gatgctgaga agctgagcag cacagacaac gaggatgagg agctggggac
     4081 agaaggctct acctcggaga agagaagccc catgaaaagg gagaggtccc gctcccacga
     4141 ctcagcatcc tcatccctct cctccaaggc ttccggttca gcgctcggtg gcgagtcctc
     4201 ggctcagccc acagcactcc cccagggaga gcatgccagg tcgccccagc cccgtggccc
     4261 cgcagaggag ggcagagccc ctggtgagaa acagaggccc cgggcaagtc aggggccacc
     4321 ctcggccatc agcaggcaca gtcccgggcc gacgccccag cccgactgta gcctcaggac
     4381 cggccagagg agcgtccagg tgtcggtcac ctcgtcgtgc tcccagctgt cctcctcctc
     4441 gggctcatcc tcctcatccg tggcgcctgc tgccggcacg tgggtcctgc aggcctccca
     4501 gtgctccttg accaaggcct gccgccagcc acccattgtc ttcttgccca agctcgtgta
     4561 cgacatggtt gtgtccactg acagcagtgg cctgcccaag gccgcctccc tcctgccctc
     4621 cccctcggtc atgtgggcca gctctttccg ccccctgctc agcaagacca tgacatccac
     4681 cgagcagtcc ctctactacc ggcagtggac ggtgccccgg cccagccaca tggactacgg
     4741 caaccgggcc gagggccgcg tggacggctt ccacccccgc aggctgctgc tcagcggccc
     4801 ccctcagatc gggaagacag gtgcctacct gcagttcctc agtgtcctgt ccaggatgct
     4861 tgttcggctc acagaagtgg atgtctatga cgaggaggag atcaatatca acctcagaga
     4921 agaatctgac tggcattatc tccagcttag cgacccctgg ccagacctgg agctgttcaa
     4981 gaagttgccc tttgactaca tcattcacga cccgaagtat gaagatgcca gcctgatttg
     5041 ttcgcactat cagggtataa agagtgaaga cagagggatg tcccggaagc cggaggacct
     5101 ttatgtgcgg cgtcagacgg cacggatgag actgtccaag tacgcagcgt acaacactta
     5161 ccaccactgt gagcagtgcc accagtacat gggcttccac ccccgctacc agctgtatga
     5221 gtccaccctg cacgcctttg ccttctctta ctccatgcta ggagaggaga tccagctgca
     5281 cttcatcatc cccaagtcca aggagcacca ctttgtcttc agccaacctg gaggccagct
     5341 ggagagcatg cgactacccc tcgtgacaga caagagccat gaatatataa aaagtccgac
     5401 attcactcca accaccggcc gtcacgaaca tgggctcttt aatctgtacc acgcaatgga
     5461 cggtgccagc catttgcacg tgctggttgt caaggaatac gagatggcaa tttataagaa
     5521 atattggccc aaccacatca tgctggtgct ccccagtatc ttcaacagtg ctggagttgg
     5581 tgctgctcat ttcctcatca aggagctgtc ctaccataac ctggagctcg agcggaaccg
     5641 gcaggaggag ctgggaatca agccgcagga catctggcct ttcattgtga tctctgatga
     5701 ctcctgcgtg atgtggaacg tggtggatgt caactctgct ggggagagaa gcagggagtt
     5761 ctcctggtcg gaaaggaacg tgtctttgaa gcacatcatg cagcacatcg aggcggcccc
     5821 cgacatcatg cactacgccc tgctgggcct gcggaagtgg tccagcaaga cccgggccag
     5881 cgaggtgcaa gagcccttct cccgctgcca cgtgcacaac ttcatcatcc tgaacgtgga
     5941 cctgacccag aacgtgcagt acaaccagaa ccggttcctg tgtgacgatg tagacttcaa
     6001 cctgcgggtg cacagcgccg gcctcctgct ctgccggttc aaccgcttca gcgtgatgaa
     6061 gaagcagatc gtggtgggcg gccacaggtc cttccacatc acatccaagg tgtctgataa
     6121 ctctgccgcg gtcgtgccgg cccagtacat ctgtgccccg gacagcaagc acacgttcct
     6181 cgcagcgccc gcccagctcc tgctggagaa gttcctgcag caccacagcc acctcttctt
     6241 cccgctgtcc ctgaagaacc atgaccaccc agtgctgtct gtcgactgtt acctgaacct
     6301 gggatctcag atttctgttt gctatgtgag ctccaggccc cactctttaa acatcagctg
     6361 ctcggacttg ctgttcagtg ggctgctgct gtacctctgt gactcttttg tgggagctag
     6421 ctttttgaaa aagtttcatt ttctgaaagg tgcgacgttg tgtgtcatct gtcaggaccg
     6481 gagctcactg cgccagacgg tcgtccgcct ggagctcgag gacgagtggc agttccggct
     6541 gcgcgatgag ttccagaccg ccaatgccag ggaagaccgg ccgctctttt ttctgacggg
     6601 acgacacatc tgaactagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     6661 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6721 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6781 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6841 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     6901 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6961 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7021 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7081 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7141 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7201 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7261 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7321 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     7381 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7441 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7501 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7561 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7621 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7681 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     7741 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     7801 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     7861 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     7921 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     7981 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     8041 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     8101 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     8161 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     8221 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     8281 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     8341 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     8401 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     8461 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     8521 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     8581 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     8641 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     8701 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     8761 gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     8821 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     8881 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8941 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9001 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     9061 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     9121 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     9181 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     9241 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     9301 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9361 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9481 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9541 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9601 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9661 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9721 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9781 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9841 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9901 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9961 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10021 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    10081 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    10141 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    10201 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    10261 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    10321 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10381 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10441 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10501 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10561 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10621 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10681 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10741 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10801 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10861 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10921 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10981 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    11041 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    11101 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    11161 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    11221 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
    11281 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
    11341 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
    11401 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
