LOCUS       sequence-216267-        6546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses myc tagged Miro2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Fransson A, Ruusala A, Aspenstrom P
  TITLE     Atypical Rho GTPases have roles in mitochondrial homeostasis and 
            apoptosis.
  JOURNAL   J Biol Chem. 2003 Feb 21;278(8):6495-502. Epub 2002 Dec 12.
  PUBMED    12482879
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2003-02-21"; volume: "278(8)"; pages: "6495-502. Epub
            2002 Dec 12"
COMMENT     Sequence Label: pRK5-myc-Miro2
FEATURES             Location/Qualifiers
     source          1..6546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    14..30
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     19..41
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     38..54
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     38..54
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        83..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     617..637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     663..687
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        897..915
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     897..914
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     928..947
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      994..1003
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1003..1032
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2749..2758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2915..2924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2928..3062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2965..2984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3019..3038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3131..3488
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3131..3322
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3154..3174)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3339..3474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3508..3525)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3508..3524)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3517..3539)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3737..4192
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3824..3843)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4034..4055
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4209..4228)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4328..4350
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4388..4406)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4474..4578
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4579..4647,4648..5439)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4797..4816)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5610..6198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6099..6118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6352..6369
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6486..6507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6522..6527,6528..6545,6546,1..6)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
       61 acgaattaat tcgagctcgc ccgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      721 catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg
      781 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacccccttg
      841 gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt
      901 aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc cactcccagg
      961 tccaactgca cctcggttct atcgattgaa ttggccacca tggagcagaa gctgatctcc
     1021 gaggaggacc tgggatctat gaggcgggac gtgcgcatcc tgttactggg cgaggcccag
     1081 gtggggaaga cgtcgctgat cctgtccctg gtgggcgagg agttccccga ggaggtccct
     1141 ccccgcgcgg aggagatcac catccccgcg gacgtcaccc cggagaaggt gcccacccac
     1201 atcgtggact actcagaagc cgagcagacg gacgaggagc tgcgggagga gatccacaag
     1261 gcaaacgtgg tgtgtgtggt gtatgacgtc tctgaggagg ccaccattga gaagattcga
     1321 actaagtgga tcccactggt gaatgggggg accacgcagg ggcccagggt gcccatcatc
     1381 ctagtgggca acaagtcaga cctgcggtcg gggagctcca tggaggccgt gctccccatc
     1441 atgagccagt tttccgagat tgagacctgc gtggagtgtt cggccaagaa cctgaggaac
     1501 atctcagagc tgttctacta cgcccagaag gccgtcctgc atcccacagc ccccctctat
     1561 gaccctgagg ccaagcagtt gaggcccgcg tgcgcccagg cgctgacgcg catcttcagg
     1621 ctctcagatc aggacctgga ccaggcgctc agtgacgaag agctcaacgc tttccagaaa
     1681 tcctgctttg ggcaccccct ggccccgcag gccctggagg acgtgaagac ggtggtgtgc
     1741 aggaacgtgg cgggcggcgt gcgggaggac cagctgaccc tggatggttt cctcttcctg
     1801 aacacgctct tcatccagcg cggccggcac gagaccacct ggaccatcct gcggcgcttc
     1861 ggctacagcg atgccctgga gctgactgcg gactatctct cccctctgat ccacgtgccc
     1921 cccggctgca gcacggagct caaccacctt ggctaccagt ttgtgcagag agtgtttgag
     1981 aagcacgacc aggaccgcga cggcgccctc tcgcccgtgg agctgcaaag ccttttcagt
     2041 gtgttcccag cagcgccctg gggccccgag ctcccacgca cagtccgcac agaggccggc
     2101 cggttgcccc tgcacggata cctctgccag tggaccctgg tgacctacct ggacgtccgg
     2161 agctgccttg gacacctagg ctacctgggc taccccaccc tctgtgagca ggaccaggcc
     2221 catgccatca cagtcactcg tgagaagagg ctggaccagg agaagggaca gacgcagcgg
     2281 agcgtcctcc tgtgcaaggt ggtaggggcc tgtggagtgg gcaagtctgc cttcctgcag
     2341 gcctttctcg gccgcggcct ggggcaccag gacacgaggg agcagcctcc cggctacgcc
     2401 atcgacacgg tgcaggtcaa tgggcaggag aagtacttga tcctctgtga ggtgggcaca
     2461 gatggtctgc tggccacatc gctggacgcc acctgtgacg ttgcctgctt gatgtttgat
     2521 ggcagtgacc caaagtcctt tgcacattgt gccagcgtct acaagcacca ttacatggac
     2581 gggcagaccc cctgcctctt tgtctcctcc aaggccgacc tgcccgaagg tgtcgcggtg
     2641 tctggcccat caccggccga gttttgccgc aagcaccggc tacccgctcc cgtgccgttc
     2701 tcctgtgctg gcccagccga gcccagcacc accatcttca cccagctcgc caccatggcc
     2761 gccttcccac atttggtcca cgcagagctg catccctctt ccttctggct ccgggggctg
     2821 ctgggggttg tcggggccgc cgtggccgca gtcctcagct tctcactcta cagggtcctg
     2881 gtgaagagcc agtgagaatt cctgcagaag cttggccgcc atggcccaac ttgtttattg
     2941 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3001 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     3061 tcgggaatta attcggcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     3121 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     3181 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3241 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3301 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3361 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3421 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3481 tttgcaaaaa gctgttaaca gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     3541 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3601 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3661 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     3721 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3781 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3841 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3901 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     3961 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4021 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     4081 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4141 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     4201 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     4261 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4321 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     4381 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     4441 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4501 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4561 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4621 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4681 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4741 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4801 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4861 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4921 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4981 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5041 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5101 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5161 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5221 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5281 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5341 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5401 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5461 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5521 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5581 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5641 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5701 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5761 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5821 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5881 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5941 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6001 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6061 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6121 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6181 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6241 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6301 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6361 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6421 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     6481 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     6541 gctcgt
//
