LOCUS       sequence-216279-        5580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa230 to C-terminus of Cre recombinase fused to PYL CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2607_pCAG-PV1-PYL-L1-iCre-230C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1217..1243
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1628..1648
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1658..1882
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2122..2141)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2187..2242
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2241..2260)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2603..2619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2603..2619)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2616..2638)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2627..2643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2651..2657,2658..2675,2676..2681))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2802..2936
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2956..3090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2993..3012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3047..3066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3158..3175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3329..3917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3409..3428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4088..4879,4880..4948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4711..4730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4949..5053)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5084..5463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg cgccaactca agacgagttc acccaactct cccaatcaat
      721 cgccgagttc cacacgtacc aactcggtaa cggccgttgc tcatctctcc tagctcagcg
      781 aatccacgcg ccgccggaaa cagtatggtc cgtggtgaga cgtttcgata ggccacagat
      841 ttacaaacac ttcatcaaaa gctgtaacgt gagtgaagat ttcgagatgc gagtgggatg
      901 cacgcgcgac gtgaacgtga taagtggatt accggcgaat acgtctcgag agagattaga
      961 tctgttggac gatgatcgga gagtgactgg gtttagtata accggtggtg aacataggct
     1021 gaggaattat aaatcggtta cgacggttca tagatttgag aaagaagaag aagaagaaag
     1081 gatctggacc gttgttttgg aatcttatgt tgttgatgta ccggaaggta attcggagga
     1141 agatacgaga ttgtttgctg atacggttat tagattgaat cttcagaaac ttgcttcgat
     1201 cactgaagct atgaactacc catacgatgt tccagattac gcttccggag ggtctggctc
     1261 cggatcaagt ggtggcagcg gtaccgtggc tgatgacccc aacaactacc tgttctgccg
     1321 ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc caactgtcca cccgggccct
     1381 ggaagggatc tttgaggcca cccaccgcct gatctatggt gccaaggatg actctgggca
     1441 gagatacctg gcctggtctg gccactctgc cagagtgggt gctgccaggg acatggccag
     1501 ggctggtgtg tccatccctg aaatcatgca ggctggtggc tggaccaatg tgaacattgt
     1561 gatgaactac atcagaaacc tggactctga gactggggcc atggtgaggc tgctcgagga
     1621 tggggacccc aagaaaaagc ggaaggtgtg agaattcctg tgccttctag ttgccagcca
     1681 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1741 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     1801 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     1861 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     1921 tatccccacg cgccctgtag cgtgtaccgc tgggagttcg tagacggaaa caaacgcaga
     1981 atccaagcgc ggccgcccag gatacataga ttaccacaac tccgagccct tccaccgcta
     2041 gcgcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     2101 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     2161 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     2221 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     2281 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     2341 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     2401 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     2461 tttttcttta acatccctaa aatttttctt acatgtttta ctagccagat ttttcctcct
     2521 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     2581 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2641 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2701 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2761 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     2821 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2881 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2941 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3001 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3061 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     3121 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3181 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3241 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3301 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3361 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3421 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3481 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3541 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3601 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3661 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3721 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3781 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3841 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3901 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3961 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4021 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4081 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4141 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4201 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4261 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4321 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4381 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4441 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4501 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4561 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4621 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4681 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4741 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4801 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4861 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4921 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4981 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5041 taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact
     5101 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     5161 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     5221 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     5281 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     5341 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     5401 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     5461 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
     5521 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
//
