LOCUS       sequence-21629-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: LTRi
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(84..102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(109..126)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(109..125)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(118..140)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    360..381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(396..401,402..419,420..426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    434..450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     439..461
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     458..474
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     458..474
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    507..628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(518..537)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     572..591
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tggaaaggac gaaacaccgc tacacaaatc agcgattttt caagagaaaa tcgctgattt
       61 gtgtagaaaa aggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt
      121 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      181 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      241 gttgcagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      301 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      361 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      421 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      481 tacgccaagc ttgggcccag acatgataag atacattgat gagtttggac aaaccacaac
      541 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      601 aaccattata agctgcaata aacaagttaa caacaacaac tgcattcatt ttatgttaca
      661 ggttcag
//
