LOCUS       sequence-21630-e         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: LTRi
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(59..78)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(60..78)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(106..125)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    569..588
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    612..631
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 agcagctcta atgcgctgtt atcactttac ttttatctaa tctagacata tcaattcgcc
       61 ctatagtgag tcgtattaca attctttgcc aaaatgatga gacagcacaa taaccagcac
      121 gttgcccagg agctgtagga aaaagaagaa ggcatgaaca tggttagcag aggggcccgt
      181 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
      241 ttagcttcct tagctcctga gcggccgcaa gcttggttgg aatctaaaat acacaaacaa
      301 ttaggatccc tgaggagcag ttctttgatt tgcaccacca ggagcctcaa atttttcaat
      361 aaattcacct gactgcacat taggactttg gctttgagct tcccacctct cagttacttg
      421 ctgaatactg tctgcttcat caatattatc ataggtgtgc ccaaatgata tttgcaaccc
      481 ttccctgttg gctacttgtc tcaccattgt aggcctaatg ggagacggat ccgtagttta
      541 acacattata cactagggtc gactgtggaa ttgtgagcgc tcacaattcc acaacctagg
      601 gtcgactgtg gaattgtgag cgctcacaat tccacagtcg accctagtgt at
//
