LOCUS       sequence-216313-        5459 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa271 to C-terminus of Cre recombinase fused to PYL CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5459
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1217..1243
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1505..1525
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1535..1759
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1999..2018)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2064..2119
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2118..2137)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2480..2496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2480..2496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2493..2515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2504..2520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2528..2534,2535..2552,2553..2558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2573..2594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2679..2813
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2833..2967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2870..2889)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2924..2943
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3035..3052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3206..3794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3286..3305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3965..4756,4757..4825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4588..4607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4826..4930)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4961..5340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg cgccaactca agacgagttc acccaactct cccaatcaat
      721 cgccgagttc cacacgtacc aactcggtaa cggccgttgc tcatctctcc tagctcagcg
      781 aatccacgcg ccgccggaaa cagtatggtc cgtggtgaga cgtttcgata ggccacagat
      841 ttacaaacac ttcatcaaaa gctgtaacgt gagtgaagat ttcgagatgc gagtgggatg
      901 cacgcgcgac gtgaacgtga taagtggatt accggcgaat acgtctcgag agagattaga
      961 tctgttggac gatgatcgga gagtgactgg gtttagtata accggtggtg aacataggct
     1021 gaggaattat aaatcggtta cgacggttca tagatttgag aaagaagaag aagaagaaag
     1081 gatctggacc gttgttttgg aatcttatgt tgttgatgta ccggaaggta attcggagga
     1141 agatacgaga ttgtttgctg atacggttat tagattgaat cttcagaaac ttgcttcgat
     1201 cactgaagct atgaactacc catacgatgt tccagattac gcttccggag ggtctggctc
     1261 cggatcaagt ggtggcagcg gtaccctgat ctatggtgcc aaggatgact ctgggcagag
     1321 atacctggcc tggtctggcc actctgccag agtgggtgct gccagggaca tggccagggc
     1381 tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg accaatgtga acattgtgat
     1441 gaactacatc agaaacctgg actctgagac tggggccatg gtgaggctgc tcgaggatgg
     1501 ggaccccaag aaaaagcgga aggtgtgaga attcctgtgc cttctagttg ccagccatct
     1561 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     1621 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     1681 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     1741 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     1801 ccccacgcgc cctgtagcgt gtaccgctgg gagttcgtag acggaaacaa acgcagaatc
     1861 caagcgcggc cgcccaggat acatagatta ccacaactcc gagcccttcc accgctagcg
     1921 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     1981 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     2041 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2101 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     2161 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     2221 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     2281 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     2341 ttctttaaca tccctaaaat ttttcttaca tgttttacta gccagatttt tcctcctctc
     2401 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     2461 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2521 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2581 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2641 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccag
     2701 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     2761 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     2821 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     2881 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     2941 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga
     3001 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3061 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4921 gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt
     4981 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     5041 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     5101 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     5161 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     5221 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     5281 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     5341 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     5401 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg ggggggggg
//
