LOCUS       sequence-216319-        5788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa27 of Flp recombinase fused to GID1 CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1835..1855
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1865..2089
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2329..2348)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2394..2449
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2448..2467)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2810..2826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2810..2826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2823..2845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2834..2850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2858..2864,2865..2882,2883..2888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2903..2924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3009..3143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3163..3297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3200..3219)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3254..3273
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3365..3382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3536..4124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3616..3635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4295..5086,5087..5155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4918..4937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5156..5260)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5291..5670
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga gccagttcga catcctgtgc aagacccccc ccaaggtgct
      721 ggtgcggcag ttcgtggaga gattcgagag gcccagctcc ggagggtctg gctccggatc
      781 aagtggtggc agcggtacca tggccgccag cgacgaagtg aacctgatcg agagcagaac
      841 cgtggtgccc ctgaacacct gggtgctgat ctccaacttc aaggtggcct acaacatcct
      901 gcggaggccc gacggcacct tcaacagaca cctggccgag tacctggacc ggaaagtgac
      961 cgccaacgcc aaccctgtgg acggcgtgtt cagcttcgac gtgctgatcg accggcggat
     1021 caacctgctg agccgggtgt acagacccgc ctacgccgat caggaacagc ccccctctat
     1081 cctggatctg gaaaagcccg tggatggcga catcgtgccc gtgatcctgt tcttccacgg
     1141 cggcagcttt gcccacagca gcgccaatag cgccatctac gacaccctgt gcagacggct
     1201 cgtgggcctg tgcaaatgcg tggtggtgtc cgtgaactac cgcagagccc ccgagaaccc
     1261 ttacccctgc gcctacgatg atggctggat cgccctgaac tgggtcaaca gcagaagctg
     1321 gctgaagtcc aagaaagaca gcaaggtgca catctttctg gccggcgata gcagcggcgg
     1381 caatatcgcc cataacgtgg ccctgagagc cggcgagtct ggcatcgatg tgctgggcaa
     1441 tatcctgctg aaccccatgt tcggcggcaa cgagcggacc gagagcgaga agtctctgga
     1501 cggcaagtac ttcgtgaccg tgcgggaccg ggactggtac tggaaggcct ttctgcccga
     1561 gggcgaggac agagagcacc ccgcctgcaa tcccttcagc cccagaggca aaagcctgga
     1621 aggcgtgtcc ttcccaaagt ccctggtggt ggtggccggc ctggacctga tcagagattg
     1681 gcagctggcc tatgccgagg gcctgaagaa agccggccag gaagtgaagc tgatgcacct
     1741 ggaaaaggcc accgtgggct tctacctgct gcccaacaac aaccacttcc acaacgtgat
     1801 ggacgagatc agcgccttcg tgaacgccga gtgccccaag aaaaagcgga aggtgtgaga
     1861 attcctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     1921 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     1981 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2041 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     2101 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgt gtaccgctgg
     2161 gagttcgtag acggaaacaa acgcagaatc caagcgcggc cgcccaggat acatagatta
     2221 ccacaactcc gagcccttcc accgctagcg cactcctcag gtgcaggctg cctatcagaa
     2281 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     2341 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     2401 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     2461 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     2521 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     2581 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     2641 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     2701 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     2761 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     2821 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2881 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2941 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     3001 aaccatagtc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3061 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3121 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     3181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     3301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5281 cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     5341 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     5401 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     5461 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     5521 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     5581 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     5641 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     5701 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     5761 tgtgcagcga tgggggcggg gggggggg
//
