LOCUS       sequence-216324-        5096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa375 to C-terminus of Flp recombinase fused to GAI CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2193_pCAG-PV1-GAI-L1-FlpO-375C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             689..706
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1142..1162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1172..1396
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1636..1655)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1701..1756
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1755..1774)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2117..2133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2117..2133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2130..2152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2141..2157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2165..2171,2172..2189,2190..2195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2210..2231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2316..2450
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2470..2604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2507..2526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2561..2580
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2672..2689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2843..3431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2923..2942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3602..4393,4394..4462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4225..4244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4463..4567)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4598..4977
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga agcgggacca ccaccatcac catcatcagg acaagaaaac
      721 catgatgatg aacgaagagg acgacggcaa cggcatggac gagctgctgg ctgtgctggg
      781 ctacaaagtg cggagcagcg agatggccga cgtggcccag aaactggaac agctggaagt
      841 gatgatgagc aacgtgcagg aagatgacct gtcccagctg gccaccgaga cagtgcacta
      901 caaccccgcc gagctgtaca cctggctgga ctccatgctg accgacctga actccggagg
      961 gtctggctcc ggatcaagtg gtggcagcgg taccaaggac gagaccaacc ccatcgagga
     1021 gtggcagcac atcgagcagc tgaagggcag cgccgagggc agcatcagat accccgcctg
     1081 gaacggcatc atcagccagg aggtgctgga ctacctgagc agctacatca acaggcggat
     1141 ccccaagaaa aagcggaagg tgtgagaatt cctgtgcctt ctagttgcca gccatctgtt
     1201 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1261 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1321 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1381 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     1441 cacgcgccct gtagcgtgta ccgctgggag ttcgtagacg gaaacaaacg cagaatccaa
     1501 gcgcggccgc ccaggataca tagattacca caactccgag cccttccacc gctagcgcac
     1561 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     1621 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     1681 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     1741 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     1801 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     1861 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     1921 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     1981 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     2041 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     2101 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2281 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccagttc
     2341 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     2401 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     2461 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2521 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2581 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag
     2641 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2701 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2761 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2821 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2881 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2941 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3001 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3061 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3121 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3181 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3241 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3301 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3361 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3421 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3481 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3541 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3601 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3661 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3721 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3781 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3841 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3901 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3961 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4021 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4081 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4141 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4201 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4261 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4321 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4381 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4441 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4501 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4561 ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat
     4621 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     4681 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4741 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     4801 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     4861 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     4921 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     4981 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     5041 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggg
//
