LOCUS       sequence-21633-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Deans TL, Cantor CR, Collins JJ
  TITLE     A tunable genetic switch based on RNAi and repressor proteins for 
            regulating gene expression in mammalian cells.
  JOURNAL   Cell. 2007 Jul 27. 130(2):363-72.
  PUBMED    17662949
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jul 27. 130(2):363-72."
COMMENT     Sequence Label: LTRi EGFP
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(118..321)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(147..167)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        322..625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcnccngatc taagcttgga naactaccta cagagattta aagctctaag gtannttgtn
       61 nnnncttana ncctgcaggt cgactctaga ggatccgatc tgacggttca ctaaaccagc
      121 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      181 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      241 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
      301 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
      361 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
      421 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
      481 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
      541 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
      601 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
      661 cgtaattgat tactattaat aactagaatt cactggccg
//
