LOCUS       sequence-21637-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Chen CM, Smith DM, Peters MA, Samson ME, Zitz J, Tabin CJ, Cepko CL
  TITLE     Production and design of more effective avian 
            replication-incompetent retroviral vectors.
  JOURNAL   Dev Biol. 1999 Oct 15. 214(2):370-84.
  PUBMED    10525341
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            1999 Oct 15. 214(2):370-84."
COMMENT     Sequence Label: pSLIRES-11 (CC#13)
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(206..222)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(207..223)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(251..270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(252..270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(277..294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(277..293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(286..308)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(522..541)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
       61 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
      121 gatgataata ccatggccat gattacgaat tcgagctcgc ccggggatcc gatatcacta
      181 gtgcggccgc tgcagccaag cttatcgata ccgtcgacct cgatcgaggg ggggcccggt
      241 acccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
      301 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      361 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      421 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      481 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      541 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      601 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agaggtgatg
      661 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      721 cgttctttta tagtggactc ttgttccaac tgggacacac tcaacctatc tcggtcta
//
